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81.
82.
Abstract: A new setup of measuring temperature is developed, which the probe is a micro- power consumptive one with CMOS circuit and is driven by optical power. For transmitting the measured signal and optical power signal in a long distance, the fiber technology is applied in this setup. 相似文献
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84.
Exploration of the two‐photon excitation spectrum of fluorescent dyes at wavelengths below the range of the Ti:Sapphire laser 下载免费PDF全文
J. TRÄGÅRDH G. ROBB R. AMOR W.B. AMOS J. DEMPSTER G. McCONNELL 《Journal of microscopy》2015,259(3):210-218
We have studied the wavelength dependence of the two‐photon excitation efficiency for a number of common UV excitable fluorescent dyes; the nuclear stains DAPI, Hoechst and SYTOX Green, chitin‐ and cellulose‐staining dye Calcofluor White and Alexa Fluor 350, in the visible and near‐infrared wavelength range (540–800 nm). For several of the dyes, we observe a substantial increase in the fluorescence emission intensity for shorter excitation wavelengths than the 680 nm which is the shortest wavelength usually available for two‐photon microscopy. We also find that although the rate of photo‐bleaching increases at shorter wavelengths, it is still possible to acquire many images with higher fluorescence intensity. This is particularly useful for applications where the aim is to image the structure, rather than monitoring changes in emission intensity over extended periods of time. We measure the excitation spectrum when the dyes are used to stain biological specimens to get a more accurate representation of the spectrum of the dye in a cell environment as compared to solution‐based measurements. 相似文献
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86.
In this work, using a fluorescent poly(phenylene ethynylene) containing the rigid pentiptycene and large cholesteryl ester pendent groups, the fluorescence quenching properties of the polymer thin films in response to vapor of 2,4-dinitrotulene (DNT) have been investigated by varying the film thickness, applying an undercoating and blending with another polar polymer. A significant change in fluorescence intensity (51% in 60 s) in response to DNT vapor exposure at ambient temperature was achieved when the polymer film coated on glass plate was about 2 nm in thickness. In comparison with the film of polymer alone, the film undercoated with (3-aminopropyl)triethoxysilane and the film of polymer blend containing a non-fluorescent polymer showed additional 18.5% (in 20-s exposure) and 18.7% (in 5-min exposure) decrease in fluorescence intensity, respectively. The use of polymer or polymer blend coated on optic-fiber tip for detection of DNT vapor has also been demonstrated. 相似文献
87.
Highly bright and photostable cyanine dye-doped silica nanoparticles for optical imaging: Photophysical characterization and cell tests 总被引:1,自引:0,他引:1
Ivana Miletto Alessandra Gilardino Pollyanna Zamburlin Simona Dalmazzo Davide Lovisolo Giuseppe Caputo Guido Viscardi Gianmario Martra 《Dyes and Pigments》2010,84(1):121-127
Spherical silica nanoparticles containing fluorescent trimethine indocyanine dyes (λabs = 547 nm, λem = 570 nm) were prepared using a water-in-oil microemulsion method. The nanoparticles were of 50 nm diameter and were almost monodispersed in aqueous solution at pH 5.5. Entrapment of dye molecules in the silica matrix stabilised photoemission over several hours of continuous irradiation. The photoemission intensity of the indocyanine was increased 13-fold over that recorded in solution. As each nanoparticle contained 110 dye molecules, the photoemission brightness of each particle was enhanced by three orders of magnitude. The fluorescent nanoparticles have been tested as imaging tools in in vitro tests. As an example of non-macrophagic cells, a highly differentiated neuronal cell line (GT1-7) was used and the results showed that the prepared nanoparticles can be incorporated into these cells with no apparent toxicity for up to three days. 相似文献
88.
《中国生物制品学杂志》2010,(10)
目的分析猪瘟病毒(Classical swine fever virus,CSFV)感染猪细胞因子和趋化因子的动态变化。方法将健康猪随机分成2组:感染组和对照组,感染组经颈部肌肉注射CSFV石门株1×103TCID50/头,对照组注射等体积的生理盐水。感染后每日观察实验猪的临床症状。分别于感染前和感染后4、6、8d采血,分离外周血白细胞和血清,应用实时荧光定量RT-PCR检测外周血白细胞IL-1、IL-4、IL-6、IL-8、IL-10、IFNγ、TNF-α和干扰素诱导蛋白10(Interferon inducible protein10,IP-10)mRNA的转录水平及全血中的病毒载量;应用ELISA法检测血清中各细胞因子的含量。结果 CSFV感染后,猪的临床症状分值逐渐升高,体温在第3天达最高,然后下降;白细胞总体呈明显下降趋势;细胞因子IL-1、IL-4、IL-6、TNF-α和IP-10的mRNA转录水平上调,IL-10的mRNA转录水平下调,IFNγ的mRNA转录水平基本未发生变化;CSFV感染猪在感染初期和症状明显期(4d和6d)的外周血的病毒载量呈明显上升趋势,而在濒死期(8d)病毒载量有所下降;CSFV感染后猪血清中的TNF-α含量上升,IL-4、IL-6、IL-8、IL-10和IFNγ的含量下降。结论猪瘟病毒感染猪TNF-α和IL-10 mRNA的转录水平与血清中的细胞因子含量变化趋势一致,而其他细胞因子mRNA的转录水平与血清中含量变化并不一致。 相似文献
89.
A simple, cost-effective yet rapid and sensitive sensor for on-site and real-time Hg2+ detection based on bovine serum albumin functionalized fluorescent gold nanoparticles as novel and environmentally friendly
fluorescent probes was developed. Using this probe, aqueous Hg2+ can be detected at 0.1 nM in a facile way based on fluorescence quenching. This probe was also applied to determine the Hg2+ in the lake samples, and the results demonstrate low interference and high sensitivity. 相似文献
90.
分别叙述了太阳能在荧光灯、HID灯、LED灯照明系统中的应用。为提高太阳能光伏照明系统的可靠性,介绍了太阳能光伏照明风光互补系统和太阳能光伏照明光电互补系统。并提出太阳能光伏照明系统中应注意的技术问题。 相似文献