A Selective,Hydrogel-Based Prodrug Delivery System Efficiently Activates a Suicide Gene to Remove Undifferentiated Human Stem Cells Within Neural Grafts

The directed differentiation of human pluripotent stem cells (hPSCs) into defined populations has advanced regenerative medicine, especially for Parkinson's disease where clinical trials are underway. Despite this, tumorigenic risks associated with incompletely patterned and/or quiescent proliferative cells within grafts remain. Addressing this, donor stem cells carrying the suicide gene, thymidine kinase (activated by the prodrug ganciclovir, GCV), are employed to enable the programmed ablation of proliferative cells within neural grafts. However, coinciding the short half-life of GCV with the short S-phase of neural progenitors is a key challenge. To overcome this, a smart hydrogel delivery matrix is fabricatedto prolong GCV presentation. Following matrix embedment, GCV retains its functionality, demonstrated by ablation of hPSCs and proliferating neural progenitors in vitro. A prolonged GCV release is measured by mass spectrometry following the injection of a GCV-functionalized hydrogel into mouse brains. Compared to suboptimal, daily systemic GCV injections, the intracerebral delivery of the functionalized hydrogel, as a “one-off treatment”, reduce proliferative cells in both hPSC-derived teratomas and neural grafts, without affecting the graft's functional unit (i.e., neurons). It is demonstrated that a functionalized biomaterial can enhance prodrug delivery and address safety concerns associated with the use of hPSCs for brain repair.     

者:扩增的造血干/祖细胞对小鼠恢复造血的作用——体内各细胞系的快速恢复、长期植入及内源性恢复,梅乐和,林东强,姚善泾,

分离雌性BDF1小鼠骨髓中单个核细胞,以MoFlo细胞分选系统分离纯化CD34+/c-kit+造血干/祖细胞,与小鼠间充质干细胞系UC-OZ-17联合体外培养后,移植于经致死剂量照射的雌性小鼠.结果表明,体外扩增有效地提高了各种细胞的扩增倍数,其中单个核细胞扩增的总有核细胞、CD34+细胞、GM-CFC和HPP-CFC扩增倍数分别为10.8、4.8、65.9和、38.8,CD34+/c-kit+细胞扩增的四种细胞扩增倍数分别为76.1、2.9、71.7和51.8.扩增细胞移植后可在体内产生嗜中性粒细胞等的快速恢复,短期快速恢复不但为长期植入提供了机会,也对内源性重建造血产生重要作用.     

自体骨髓干细胞移植与归元方联用治疗急慢性肝损伤实验研究

研究中药归元方与自体骨髓干细胞移植对急慢性肝损伤的治疗作用.研究方法:用肝脏局部注射乙醇的方法复制急性局限性肝损伤模型,复合因素(CCl4、乙醇、高脂、低蛋白)刺激复制大鼠肝纤维化模型,通过定量组织学、肝功能检查、免疫组化、肝组织羟脯氨酸含量、损伤或纤维区骨髓干细胞观察等综合评价中药、自体骨髓干细胞移植及两者合用的疗效.结果:归元方与自体骨髓干细胞移植可减小肝损伤区域,改善肝功能,使纤维肝组织表达μPA增强,降低血清ALT,AST,PCⅢ,HA和肝组织羟脯氨酸的含量,改善肝组织肝纤维化评分,骨髓干细胞能在肝损伤、肝纤维化形成环境中存活、增殖,并向肝细胞分化,表达肝脏特异的角蛋白CK18.结论:归元方与自体骨髓干细胞移植对急慢性肝损伤有明确的治疗作用,两者合用可优势互补,协同增效.临床上有良好的应用前景.     

Technological and Medical Advances: Implications for Health Psychology.

Behavioral telehealth, health informatics, organ and tissue transplantation, and genetics are among the areas that have been affected by advances in technology and medicine. These areas illustrate the opportunities and the challenges that new developments can pose to health psychologists. Each area is discussed with respect to implications for practice, research, public policy, and education and training: recommendations are provided. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

嵌入式Web服务器下μClinux移植研究

以开发嵌入式Web服务器为目标,搭建了移植μClinux需要的开发环境和设计移植的流程,并对μClinux移植进行裁减和分析。bootloader、μClinux内核和只读的romfs文件系统烧到Flash中的分析研究及μClinux内核和根文件系统的烧到Flash详细过程描述。     

基于 S3C2440 的 Uboot 分析与移植

首先根据对Uboot的结构功能和启动分析,提出了一种基于s3c2440大容量Nand Flash和Nor Flash的移植方案,然后通过多步的移植,完善各个功能模块。最终在Uboot下,使用NFS方式成功加载内核和文件系统,详细给出了Uboot移植方法。     

基于Qt/Embedded的嵌入式GUI的研究与构建

嵌入式系统在手机、PDA等手持信息设备领域的应用出现了强劲的发展势头。而一个十分友好的图形用户界面(GUI)是嵌入式系统设计的关键技术之一,针对这种GUI的开发应用软件有很多,Qt/E是其中比较流行的一种。首先分析了Qt/E的运行机制及特点,并详细阐述其在基于ARM的嵌入式Linux系统下的移植方法,最后使用一个简单的应用程序来说明Qt/Embedded的嵌入式GUI的开发流程。     

STM32平台的μC/GUI移植与图形界面设计

μC/GUI是一种专为嵌入式应用设计的通用图形接口软件。本文详细介绍了μC/GUI的结构框架和基于STM32平台的μC/OS-Ⅱ上的μC/GUI移植过程,并在此基础上进行图形界面设计;阐述了μC/GUI的窗口管理机制,提出了实现μC/GUI中文小型字库的两种方法并采用存储设备解决了图形显示的闪烁问题。     

Induction of immune tolerance with heart-thymus composite allotransplantation in rats

Objective To study on the role of thymus transplantation for heart allograft in rats. Methods Vascularized heart-thymus combined transplantation was performed with microsurgical technique. Graft survival, histopathology, level of IL-2, IL-4 and its mRNA expression in serum and cardiac grafts were investigated. Results Heart-thymus combined transplantation achieved effect in the prolongation of cardiac graft survival with short-term administration of cyclosporine. Conclusions Vascularized thymus transplantation induced immune tolerance in thymectomized rats.     

端元选择算法在波段选择中的应用

冗余信息的大量存在,给高光谱数据的分析和处理带来很大的困难。波段选择能够有效地去除由于高光谱图像较高的数据维导致的冗余信息,从而减少计算量。高光谱图像处理的另一重要技术——端元选择到波段选择存在着方法上的可移植性。作者经过可行性分析,将3种典型的端元选择算法应用于波段选择之中,并通过引入自动子空间分解、核主成分分析、距离等效原则等内容来解决由此带来的相关问题。仿真实验证明了这种算法移植的有效性。