Advancing Small‐Molecule‐Based Chemical Biology with Next‐Generation Sequencing Technologies

Next‐generation‐sequencing (NGS) technologies enable us to obtain extensive information by deciphering millions of individual DNA sequencing reactions simultaneously. The new DNA‐sequencing strategies exceed their precursors in output by many orders of magnitude, resulting in a quantitative increase in valuable sequence information that could be harnessed for qualitative analysis. Sequencing on this scale has facilitated significant advances in diverse disciplines, ranging from the discovery, design, and evaluation of many small molecules and relevant biological mechanisms to maturation of personalized therapies. NGS technologies that have recently become affordable allow us to gain in‐depth insight into small‐molecule‐triggered biological phenomena and empower researchers to develop advanced versions of small molecules. In this review we focus on the overlooked implications of NGS technologies in chemical biology, with a special emphasis on small‐molecule development and screening.     

核酸适配体在样品前处理技术中的应用

核酸适配体是通过指数富集配体系统进化技术经体外筛选得到的可以特异性地识别目标物的一段短的单链寡核苷酸序列。与抗体相比,适配体具有性质稳定、易化学合成、易化学修饰、分子量小和目标分子广泛等优点,目前在生物传感器、毛细管电泳、物质分离富集以及医疗诊断等领域得到了广泛应用。本文重点介绍了核酸适配体在固相萃取、磁分离、亲和色谱及微流控分离分析等样品前处理技术中的应用,并对其应用现状和发展前景进行了综述。