Preparation of fatty acid methyl esters for gas-chromatographic analysis of lipids in biological materials

Theoretically, preparation of fatty acid methyl esters (FAMEs) deals with reversible chemical reactions in a complex system. Methodologically, there are numerous ways, generally characterized by the type of catalysts used and steps involved. Although there are more than a half dozen common catalysts, the majority fall into either acidic (HCl, H₂SO₄ and BF₃) or alkaline types (NaOCH₃, KOH and NaOH), with each having its own catalytic capability and application limitations. In terms of steps, many conventional methods, including those officially recognized, consist of drying, digestion, extraction, purification, alkaline hydrolysis, transmethylation/methylation and postreaction work-up. Although these methods are capable of providing reliable estimates if some precautions are taken, they are cumbersome, time-consuming and cost-inefficient. A new approach has been to transmethylate lipidsin situ. Due to its simplicity, high sensitivity, comparable reliability and capability to determine total fatty acids, the method of direct transmethylation is finding a unique place in lipid determination. Regardless of which method is used, quantitative methylation requires chemists to take precautions at every step involved, particularly during FAME formation and subsequent recovery steps. Evidently, there is an urgent need for more systematic studies, guided by the chemical principle of reactions involved and physicochemical properties of regents and end products, into factors affecting these steps. Hopefully, this will lead to an improved method, which measures lipid composition in biological materials not only with high accuracy but also with high efficiency and minimum costs.     

脉冲功率器件直流和动态热特性探测

介绍了针对影响微波脉冲功率器件可靠性的热性能问题，应用红外热分析技术。对比探测微波脉冲功率器件在动态和直流状态下的温度分布，峰值结温，热阻及其变化规律。     

Properties of ligno‐cellulose fiber Hildegardia

Studies on some properties such as the density, the degradation temperatures, the morphology and the spectral features of the ligno‐cellulose fiber Hildegardia were carried out in both untreated and alkali treated form. The fibers are found to have good morphology and moderate initial and final degradation temperatures. On alkali treatment, the lignin was found to be eliminated. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 84: 2216–2221, 2002     

TRT入口方管道爆裂原因剖析

介绍了鞍钢7#高炉TRT装置入口方管道爆裂事故经过,进行了原因分析,并提出了解决措施。     

测井资料高分辨率层序地层分析

高分辨率层序地层学克服了岩性地层对比穿时的缺点.高分辨率层序地层对比是等时的高精度的地层对比,它满足了勘探开发中地层对比等时性和高分辨率的要求.测井资料的纵向分辨率较高,是高分辨率层序地层研究的重要资料.提出了利用测井相分析技术,从测井资料上识别出单井岩性和沉积微相剖面,结合测井曲线特征,划分单井基准面旋回,并进行连井地层等时对比,这为定量地进行高分辨率层序地层分析提供了一种方法.通过研究区实际资料的处理,验证了该方法的有效性.     

旅大10-1油田化学驱物理模拟研究

旅大10-1油田位于渤海辽东湾中部海域,油藏具有高温、高矿化度和胶结疏松等特点。从海上油田开发实际需求出发,结合油藏地质特征和流体性质特点,利用室内物理模拟实验方法,研究了早期注聚合物和聚合物微凝胶的驱油效果。结果表明,早期化学驱不仅大大缩短油田开发周期、节约操作费用,而且最终采收率也高于中后期化学驱的采收率。结果还表明,在化学药剂费用相同条件下,聚合物凝胶驱的增油效果要好于普通聚合物驱。     

二次型时频分析技术的开发与应用

基于二次型的时频分析是高分辨率时频分析技术的有效方法之一，该项技术能够有效反映时变信号的频谱特性，具有分辨率高、能量集中和跟踪瞬时频率的特点。从时变信号的特点出发，阐述了二次型时频分析技术的基本方法原理，采用正演模型分析验证了该方法的有效性，并在实际地震资料中应用于识别物源方向、划分沉积相带、预测储层结构以及相对厚度等诸多方面，均取得了较好的效果。     

Hydro-economic Modeling in River Basin Management: Implications and Applications for the European Water Framework Directive

Economic ideas and processes are becoming increasingly integrated with more traditional engineering and hydrologic models of water management problems. Combining economic management concepts and performance indicators with an engineering-level of understanding of a hydrologic system can provide results and insights more directly relevant for water management decisions and policies. When such models are developed and used with involvement of stakeholders, they can become a basis for shared understanding of water problems as a foundation for negotiated management and policy solutions. When implemented with optimization software, integrated hydro-economic models also can suggest promising innovative solutions for policy-makers to consider. Their applications to river basin management problems are reviewed. Economic and integrated economic-engineering-hydrologic modeling is then discussed in the context of the evolving European Water Framework Directive. Relevant items are cost recovery and water pricing, cost-effectiveness of water management measures, and public participation in decision processes.     

Physical degradation of genomic DNA of soybean flours does not impair relative quantification of its transgenic content

In this paper, four different physical treatments (microwaves, heating by conduction, sonication and pressure autoclaving) were performed to degrade a pure DNA extract, and their influence on GMO quantification was studied. The aim was to check the hypothesis that processing of agrofood products results in a similar degradation rate for both the transgenic target and the specific target. Indeed we could observe that even if the used physical treatments could lead to a significant increase of C _t values for both transgenic and specific targets, the resulting ΔC _t remained stable. So, the main conclusion of the study is that the aforementioned hypothesis seems valid and thus a physical degradation of DNA will not affect the relative quantitation of the GMO content, provided that both the specific and transgenic targets have very similar size. A second important issue of the experiments performed was that DNA is a very robust molecule as it is extremely difficult to reach a mean size below 100 bp. The study also gives evidence of the importance of using small targets.     

大沙河护岸生态效果的数值模拟

利用River2D软件模拟了大连市大沙河多孔栖息单元式生态护岸修建后的水流状况和鱼类栖息地条件。为了选定护岸铺设的位置,以便达到最佳的河流恢复效果,把护岸铺设分为4种方案:方案一维持原貌,不铺设护岸;方案二在整个河段沿岸修建护岸;方案三在需要保护的地方修建河流护底;方案四护岸与护底同时修筑。对比了4种方案下鲤鱼流速适宜指数和河段权重可利用面积,指出了在不同流量下的护岸最佳铺设方式。结果表明利用软件进行数值模拟的方法可以弥补室内实验方法和实地观测方法的不足。