Ultrathin and Switchable Nanoporous Catalytic Membranes of Polystyrene‐b‐poly‐4‐Vinyl Pyridine Block Copolymer Spherical Micelles

An easy fabrication of close‐packed and block copolymer micelles‐based ultrathin membranes for water purification, separation, catalytic, and dye degradation applications is reported. Nanoporous membranes based on the self‐assembly of 2‐(4′‐hydroxybenzeneazo) benzoic acid (HABA)‐polystyrene‐b‐poly(4‐vinylpyridine) (PS‐b‐P4VP) diblock copolymers supramolecular complexes are prepared by simple spin coating on pore‐filled polyethylene terephthalate (PET) track‐etched membranes. The prepared membranes are characterized by scanning electron microscopy, atomic force microscopy, transmission electron microscopy, and water permeation studies. The separation performance is studied by lysozyme protein rejection. The prepared membranes are also used to in situ synthesize gold nanoparticles in the corona of PS‐b‐P4VP spheres for catalytic activity towards the reduction of p‐nitrophenol and degradation of congo red dye in flow through operation mode in a stirred cell membrane reactor. More than 95% reduction for p‐nitrophenol and >98% degradation of Congo red at a sufficiently high flux indicates its suitability for catalytic transformation and environmental remediation applications.     

实时荧光PCR方法检测副溶血性弧菌的研究

建立了一种快速检测副溶血性弧菌（vibrio parahaemolyticus,VP）的荧光PCR（Real time polymerase chain re-action）方法。首先,从GenBank中获得副溶血性弧菌种特异性基因不耐热溶血毒素基因tl和直接耐热溶血素毒素基因tdh,用Primer Express2.0设计引物和Taqman荧光探针,在Roche荧光PCR上进行荧光PCR扩增,荧光曲线表明该荧光PCR可特异性地检测副溶血性弧菌,而大肠杆菌等其他14种细菌和空白对照都是阴性;检测方法灵敏度达10cfu/reaction。本研究建立的荧光PCR检测副溶血性弧菌的方法快速、特异性强,灵敏度高,稳定性好,可检测出总的和带tdh毒力基因的副溶血性弧菌,适合于大批量样品的检验,可广泛用于出入境检疫和动物防疫监督部门的疫情监测。     

重组传染性法氏囊病病毒VP2/VP243基因表达及保护性和免疫原性

目的在重组鸡痘病毒中表达传染性法氏囊病毒 VP2/VP243基因,并研究表达产物的保护性和免 疫原性。方法以 FPV 282E_4株 TK基因为侧翼,分别将鸡法氏囊病病毒(Infectious Bursal Disease Virus,IBDV) VP2 和VP243目的基因克隆到牛痘病毒A型包涵体(ATI)和痘苗病毒P7.5复合型启动子下游,构建成2个重组表达质 粒,命名为pUTALacVP和pUTA LacVPO。用这2个质粒转染CEF细胞,用X-gal染色法筛选出2株重组病毒vUTA lacVP2和 vUTALacVP0。用这 2株病毒免疫 1周龄 SPF鸡,以常规疫苗为对照。结果 ELISA、SDS-PAGE和 Western blot表明表达产物可与IBDV特异性多克隆抗体反应,并诱导鸡保护性抗体。在使用vvIBDV攻击前,对照组抗体水 平显著高于实验组。但攻击5 d后,实验组抗体水平明显升高。结论vUTALacVP2和 VPO在 CEF细胞中实现了高 效表达,但重组疫苗的保护率低于常规疫苗。     

Studies on rubber‐to‐nylon tire cord bonding

Nylon tire cord (1680/2) was dipped in different adhesives based on resorcinol formaldehyde resin and latex (RFL) and was bonded to natural rubber‐based compounds. The resin‐rubber ratio in the RFL adhesive was optimized. The variation of pull‐through load was studied by varying the drying and curing temperature of the dipped nylon tire cord. RFL adhesive based on vinylpyridine latex was found to have better rubber‐to‐nylon tire cord bonding, compared with the one based on natural rubber latex. Addition of a formaldehyde donor into the RFL adhesive/rubber compound improves adhesion. © 1999 John Wiley & Sons, Inc. J Appl Polym Sci 71: 1197–1202, 1999     

5A和VP800分子筛的氮氧分离性能

在298.15 K下,分别测定了5A和VP800分子筛的静态吸附等温线和动态穿透曲线,并根据动态穿透曲线分别计算出5A和VP800分子筛的氮氧分离系数。研究发现,VP800分子筛的氮氧分离选择性明显高于5A分子筛。从动态吸附的结果来看,VP800有一个最优的吸附分离压力。     

基于虚拟样机技术的液压支撑平台动力学仿真

通过分析液压系统以及系统载荷的数学模型,在ADAMS环境下建立了某液压支撑平台系统的虚拟样机模型。在虚拟样机的基础上仿真、分析和优化系统动态响应,为实际系统的设计和制造提供了参数依据。     

GⅡ.4型诺如病毒VP2蛋白生物信息学分析

为了解诺如病毒VP2蛋白的生物学特征，该研究以当前诺如病毒GII.4型流行毒株VP2蛋白为研究对象，应用生物信息学软件对VP2蛋白的理化性质、磷酸化位点、糖基化位点、跨膜区、信号肽、二级结构、三级结构、抗原决定簇和B细胞抗原表位进行预测分析。结果表明，诺如病毒流行毒株VP2蛋白为稳定的亲水性蛋白，平均等电点为10.47、吸水系数为-0.47、不稳定系数为47.91，碱性氨基酸约占含量的11.60%；该蛋白含有约43个潜在的磷酸化修饰位点和2个潜在的糖基化修饰位点；大多数毒株不存在跨膜区和信号肽，然而GI.3型诺如病毒VP2蛋白存在跨膜区和信号肽；诺如病毒VP2蛋白二级结构中α-螺旋平均占比38.17%、延伸链平均占比7.45%、β-折叠平均占比6.25%、无规则卷曲平均占比48.13%；三级结构预测模型的蛋白覆盖率为56.70%；平均存在8个潜在的蛋白质抗原决定簇和25个B细胞抗原表位。该研究运用生物信息学分析方法预测当前GII.4型诺如病毒流行毒株VP2蛋白的理化性质和结构等方面，为进一步深入研究诺如病毒VP2蛋白功能奠定了理论基础。