长输管道建设物流管理新模式——物资配送

长输管道工程是以线路管道与沿途站场为主要内容的建设工程,其物资配送有着不同于一般物流管理的特点,点多线长,且交通不便.文章结合西南成品油管道建设工程的物资配送实践,总结出长输管道物资配送的类型,并从机构设置、计划编制、台账和报表管理等方面介绍了物资配送的管理内容及经验,实践证明,这种物资配送方法不但使业主的物资管理人员大为减少,而且使长输管道工程的物资供应更加及时有序.     

浅谈湖滨带生态恢复建设工程施工监理的特点

指出湖滨带是连接湖泊水体和陆地的一个功能过渡界面区。它作为生物的重要栖息地,具有净化水质、缓解波浪等多种功能。因此,对湖滨带实行生态恢复建设,应予以高度的重视。笔者通过具体工程的实践,阐述了湖滨带生态恢复建设工程的特点、施工阶段的监理工作,以及对监理人员的素质要求,可供类似工程借鉴、参考。     

试论就业形势与战略

论述了就业的特殊重要性、目前我国就业形势的极端严重性，以及所应当采取的战略和措施。     

混凝土U形渠道施工

混凝土U形渠施工的主要工序为施工前准备、U形渠施工、混凝土养护、伸缩缝填料、渠堤清理、边坡整修等。要严格控制各工序施工质量，确保U形渠施工质量，使U形渠发挥正常的效益。     

针梁台车在大花水水电站引水隧洞混凝土衬砌施工中的改进

大花水水电站引水隧洞由于前期开挖工期略有滞后，为加快施工进度，结合全圆断面的施工特点，大部分引水隧洞采用了全圆针梁穿行式钢模台车进行混凝土衬砌。通过各参建单位对针梁台车的运用和改进，有效地提高了引水隧洞混凝土的衬砌速度，并确保了工程质量。     

混凝土喷锚技术在宝珠寺水电站边坡防护中的应用

混凝土喷锚支护可提高边坡岩土的结构强度和抗变形刚度,增强边坡的整体稳定性和承载能力.本文根据宝珠寺水电站左右岸边坡主要采用混凝土喷锚支护的工程实践,介绍了施工方案设计、材料要求、施工方法、技术措施,以及现场质量管理和检测等问题.     

Use of a YAP1 overexpression cassette conferring specific resistance to cerulenin and cycloheximide as an efficient selectable marker in the yeast Saccharomyces cerevisiae.

Drug-resistance markers for yeast transformation are useful because they can be applied to strains without auxotrophic mutations. However, they are susceptible to technical difficulties, namely lower transformation efficiency and the appearance of drug-resistant mutants without the marker. To avoid these problems, we have constructed a phosphoglycerate kinase (PGK) promoter-driven YAP1 expression cassette, called PGKp-YAP1. Yeast cells containing PGKp-YAP1 were resistant to cycloheximide, a protein synthesis inhibitor, and also to cerulenin, a fatty acid synthesis inhibitor, but not to other drugs tested. The transformation efficiency of PGKp-YAP1 using cerulenin selection was comparable to that using a URA3 auxotrophic marker when low concentrations of cerulenin were used. Non-transformed drug-resistant colonies did appear on the low-concentration cerulenin plates. However, these non-transformed colonies could easily be identified, based on their cycloheximide sensitivity and/or their resistance to aureobasidin A to which the transformants were sensitive. Therefore, the dual drug resistance of PGKp-YAP1 could be used as an effective selection for PGKp-YAP1 recipient cells. The PGKp-YAP1 marker was used to disrupt the LYS2 gene and to transform an industrial yeast strain, indicating that this marker can be used for efficient and reliable gene manipulations in any Saccharomyces cerevisiae strain.     

鞍钢1780mm热轧生产线"三电"工程施工概述

概述了鞍钢1780mm生产线技术改造工程中供电、传动及自动控制系统的构成、安装及调试技术要点,以及技术经济效果评述,可供同类工程施工参考.     

油气井射孔器用炸药及其安全性

介绍了中国和美国射孔器所用的单质和混合炸药43种.根据耐热性,射孔器可分为普通型、高温型和超高温型三类.射孔器和炸药的耐热性与其受热时间有关,最后文中叙述了射孔器的安全性能.