HSV-tk基因/GCV系统治疗肿瘤的旁观者效应与缝隙连接关系的研究

目的 探讨单纯疱疹病毒胸苷激酶（HSV－tk）基因／丙氧鸟苷（GCV）系统杀伤肿瘤细胞时旁观者效应与细胞间缝隙连接的关系。方法 用PA317细胞包装STK质粒（逆转录病毒载体介导的HSV-tk基因），形成假逆转录病毒颗粒，转染有缝隙连接的大鼠胶质瘤细胞C6和无缝隙连接的入宫颈癌细胞 Hela，制成 C6 tk+和 Helatk+细胞，将不同比例的C6和 C6 tk+、Hela和 Hela tk+细胞混合，每种比例8孔，培养 24h后 4孔加入含0．5μg/mlGCV的培养基，4孔作对照，6d后用MTT法测量细胞存活率。在两个培养皿中放入周围粘有33mm高滤纸的载玻片，分别接种 C6、C6 tk+细胞。细胞 80%汇合后将载玻片互换，并加入含0．5μg/ml GCV的培养基，6d后观察细胞形态。结果C6细胞组存在旁观者效应，Heal细胞组不存在旁观者效应。培养皿中tk+细胞大部分被杀死,tk-细胞无变化。结论HSV-tk基因/GCV系统杀伤肿瘤细胞时旁观者效应与细胞间缝隙连接有关。     

多变量鲁里叶系统鲁棒输入、输出稳定性的Gap度量界

本文讨论了多变量鲁里叶系统鲁棒输入，输出稳定性问题，文中利用Ｇａｐ度量作为系统中非结构型摄动的测量手段，给出了当系统线性部分的摄动量和Ｇａｐ度量满足某种估计式，同时非线性部分的扇形斜率也受到某种类型摄动时，闭环系统仍能保持稳定的若干充分条件。     

大电流真空电弧特性的研究与分析

本文分析并介绍了大电流时纵向磁场，触头开距，触头半径等对电弧特性的影响，可为高压大容量直空断路器设计提供参考。     

Y3系列三相异步电动机设计--冷轧硅钢片冲模模具间隙的选取

模具间隙是决定冲片质量的主要因素之一,根据冷轧硅钢片的机械性能,提出了选取冷轧硅钢片冲模模具间隙的方案.     

A Study of the flow phenomenon of water in a channel with flat plate obstruction geometry at the entry

The flow ina parallel walled test channel, when obstructed with a geometry at the entrance, can be forward, reverse and stagnant depending on the position of the obstruction. This interesting flow phenomenon has potential benefit in the control of energy and various flows in the process industry. In this experiment, the flat plate obstruction geometry was used as an obstruction at the entry of the test channel. The parameters that influence the flow inside and around the test channel were the gap (g) between the test channel and the obstruction geometry, the length (L) of the test channel and the Reynolds number (Re). The effect of the gap to channel width ratio (g/w) on the magnitude of the velocity ratio (V₁/V₀ : velocity inside/ velocity outside the test channel) was investigated for a range of Reynolds numbers. The maximum reverse flow observed was nearly 20% to 60% of the outside velocity for Reynolds number ranging from 1000 to 9000 at g/w ratio of 1.5. The maximum forward velocity inside the test channel was found 80% of the outside velocity at higher g/w ratio of 8. The effect of the test channel length on the velocity ratio was investigated for different g/w ratios and a fixed Reynolds number of 4000. The influence of the Reynolds number on the velocity ratio is also discussed and presented for different gap to width ratio (g/w). The flow visualisation photo-graphs showing fluid motion inside and around the test channel are also presented and discussed.     

宽厚板热矫直机辊缝设定及控制

宽厚板热矫直机的辊缝控制系统是热矫直机最重要的系统，辊缝的设定和反馈直接影响钢板矫直效果，通过研究辊缝的计算方法，可以更好消除钢板的变形。     

从青浦到嘉定 大都市无序蔓延中的先进规划

随着空前规模与速度的城市化进程,在上个十年中,上海周边的乡村地区发生了惊人的变化。在离散化的政策下,许多新城与郊区依次落成。每一个地区独特的物质条件与规章制度形成新区难以预计的多样性,同时也造成空间与功能性品质的多样化。大多数新区都依据标准步骤进行开发。一些试点新城的开发通过文本形式在西方设计师的帮助下适应中国的规范;另一些新城与街区由来自中国的新锐建筑师与规划师设计。本文主要介绍嘉定与青浦区的发展成果。     

大间隙钎焊在燃气轮机热端部件修复领域的应用和发展

综述了钎焊修复技术在燃气轮机高温部件修复领域的研究进展,介绍了国内外一些先进的大间隙钎焊技术(WGB)修复方法,深入分析了不同修复方法的特点。探讨了WGB修复技术存在的一些问题并对未来的发展进行展望。     

The 2013 reforms of the Flemish renewable electricity support: Missed opportunities

The Flemish renewable electricity support system has struggled to address a number of problematic issues in the past. These included excessive profit margins and general malfunctioning of the green certificate market, as well as a lack of qualification of various existing renewable energy technologies. The Flemish government responded to these issues by introducing major reforms in 2013, including “banding” to differentiate the support for various technologies. However, reliable methods for differentiating renewable electricity technologies and calculating support levels have not been sufficiently developed. The main objective of the 2013 reforms was to reduce support costs, but application of German feed-in tariffs on 18 reference technologies has shown that most projects in Flanders continue to receive high levels of support. The 2013 reforms did not succeed in addressing malfunctioning of the green certificate market. On the contrary, the confidence of investors in renewable electricity plants has decreased as the terms of support can be altered retroactively by adjusting remuneration levels and through political interventions. Future adaptations are likely to be made which will further decrease the overall stability and effectiveness of the system.