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741.
Various factors, including starch granule channels, have been suggested to contribute to the control of sorghum starch digestibility for animal feed. Isolated starch from two normal sorghum lines (P721N, IS6986) and one high protein digestibility (HPD) mutant line (111) that differed in starch granule morphology were selected to study the influence of these factors on starch digestibility. Scanning electron micrographs were taken of raw and digested starches. Microscopy results confirmed that in all three sorghum lines channels in starch granules are the main route of enzyme penetration and the central cavity area is the starting point of enzyme digestion. Channel density was more pronounced in the HPD sorghum mutant line than in normal lines, which may have been responsible for its relatively high digestibility. Micrographs of IS6986 showed unique starch granule morphology with a collapsed ”doughnut‐shaped” structure in a portion of the granules. These unusual granules were rapidly digested and, unlike normal spherical granules, totally disappeared after 30 min of digestion. Amylases appeared to have fast access to the collapsed‐appearing starch granules. Digestion profiles, following incubation with pepsin and α‐amylase, showed that IS6986 and the HPD mutant (111) had the highest initial rate of starch digestion, followed by P721N. These findings provide insight as to how new sorghum cultivars might be developed with high starch digestibility for animal feed use. 相似文献
742.
Kittiwut Kasemwong Kuakoon Piyachomkwan Rungtiva Wansuksri Klanarong Sriroth 《Starch - St?rke》2008,60(11):624-633
Small and large granule fractions were isolated from canna starch (Canna edulis, green leaf cultivar), and their morphology, physicochemical properties, susceptibility towards granular starch hydrolyzing enzymes and chemical reaction with propylene oxide were investigated. Canna starch consisted of a mixed population of large, medium and small granules; the mean of granule diameter was 47.4 μm. The small granules presented round and polygonal shapes, whereas the large granules had oval and elliptical shapes. Significant variations in digestibility of the various granules size by granular starch hydrolyzing enzymes were observed. During the first 24 h, the hydrolysis rate of small granules was higher than that of native and large granule starches. After 72 h, however, the degree of hydrolysis of small granule, large granule and native starches had reached the extent of 19.6%, 32.0% and 27.2%, respectively. The larger the granule size, the higher the MS obtained when modified with propylene oxide, which was due to the higher swelling power of the large granules. The results obtained from this study suggest that small granules had lower water and chemical affinity when compared with the bigger ones. The difference in the reactivity of small and large granules could be presumably attributed to the starch components (amylose and amylopectin) and their organization of glucan chains in ordered and/or less ordered structure of these two fractions. 相似文献