Burkholderia thailandensis E264/pBMTL3-tdpR发酵生产抗癌药物ThailandepsinA的研究

在伯克氏菌Bth264野生株产新型抗癌药物Thailandepsin A以及调节基因tdp R正向调控Thailandepsin A生物合成的基础上,利用基因工程菌Bth264/p BMTL3-tdp R发酵生产Thailandepsin A,以提高产量。以0.5%乳糖为诱导剂,确定最佳诱导条件:发酵15 h添加乳糖,诱导时间6 h;通过单因素实验,确定葡萄糖和胰蛋白胨作为碳氮源、装液量65/250 m L以及接种量1%;同时结合优化发酵培养基进行发酵,Thailandepsin A产量达到252.14 mg·L-1,比优化前的产量提高56%;另外在发酵过程中,添加大孔树脂HP-20原位吸附产物,Thailandepsin A产量可达283.75 mg·L-1,比不加树脂提高13.8%;最后,基于RT-PCR和比较Ct值法,基因工程菌和野生菌相比,Thailandepsin A生物合成基因tdp B、tdp C1的转录水平分别提高11.4倍和6.0倍,对应的产量增加4.6倍,从而在很大程度上说明调节基因tdp R的过表达促进生物合成基因转录水平的提高以及产量的增加。     

Quality and Emulsion Stability of Milk from Ettawah Crossed Bred Goat During Frozen Storage

Goat milk has specific chemical composition and physical properties. The purpose of this study was to investigate the change of milk quality and emulsion stability during 60 d frozen storage. Milk sample was taken from three Ettawah Crossedbred goats that were divided into three groups. Samples were frozen and stored for 0, 30 and 60 days. At the end of each storage periode, milk sample was thawed in the refrigerator and analyzed for total number of bacteria, chemical (acidity, pH, free fatty acids), physical (alcohol test, clot on boiling test) quality, and emulsion stability. The microbiological and chemical data were analyzed statistically using one way ANOVA, whereas physical quality and emulsion stabilty were expressed qualitatively. There were no change in the total bacteria, acidity, pH and free fatty acid (FFA) of milk during storage, whereas the assessment by 70% alcohol showed positive since 0 d. Emulsions stability changed after 30 d of storage. While, the clot on boiling (COB) test of milk was positive at 60 d of storage. Recommendation is frozen storage of goat milk should not longer than 30 d.     

Stream-Based Data Sampling Mechanism for Process Object

Process object is the instance of process. Vertexes and edges are in the graph of process object. There are different types of the object itself and the associations between object. For the large-scale data, there are many changes reflected. Recently, how to find appropriate real-time data for process object becomes a hot research topic. Data sampling is a kind of finding c hanges o f p rocess o bjects. There i s r equirements f or s ampling to be adaptive to underlying distribution of data stream. In this paper, we have proposed a adaptive data sampling mechanism to find a ppropriate d ata t o m odeling. F irst o f all, we use concept drift to make the partition of the life cycle of process object. Then, entity community detection is proposed to find changes. Finally, we propose stream-based real-time optimization of data sampling. Contributions of this paper are concept drift, community detection, and stream-based real-time computing. Experiments show the effectiveness and feasibility of our proposed adaptive data sampling mechanism for process object.     

A New NTRU-Type Public-Key Cryptosystem over the Binary Field

As the development of cloud computing and the convenience of wireless sensor netowrks, smart devices are widely used in daily life, but the security issues of the smart devices have not been well resolved. In this paper, we present a new NTRU-type public-key cryptosystem over the binary field. Specifically, the security of our scheme relies on the computational intractability of an unbalanced sparse polynomial ratio problem (DUSPR). Through theoretical analysis, we prove the correctness of our proposed cryptosystem. Furthermore, we implement our scheme using the NTL library, and conduct a group of experiments to evaluate the capabilities and consuming time of encryption and decryption. Our experiments result demonstrates that the NTRU-type public-key cryptosystem over the binary field is relatively practical and effective.     

Super-Resolution Reconstruction of Images Based on Microarray Camera

In the field of images and imaging, super-resolution (SR) reconstruction of images is a technique that converts one or more low-resolution (LR) images into a highresolution (HR) image. The classical two types of SR methods are mainly based on applying a single image or multiple images captured by a single camera. Microarray camera has the characteristics of small size, multi views, and the possibility of applying to portable devices. It has become a research hotspot in image processing. In this paper, we propose a SR reconstruction of images based on a microarray camera for sharpening and registration processing of array images. The array images are interpolated to obtain a HR image initially followed by a convolution neural network (CNN) procedure for enhancement. The convolution layers of our convolution neural network are 3×3 or 1×1 layers, of which the 1×1 layers are used to improve the network performance particularly. A bottleneck structure is applied to reduce the parameter numbers of the nonlinear mapping and to improve the nonlinear capability of the whole network. Finally, we use a 3×3 deconvolution layer to significantly reduce the number of parameters compared to the deconvolution layer of FSRCNN-s. The experiments show that the proposed method can not only ameliorate effectively the texture quality of the target image based on the array images information, but also further enhance the quality of the initial high resolution image by the improved CNN.     

Controlled Secure Direct Communication Protocol via the Three-Qubit Partially Entangled Set of States

In this paper, we first re-examine the previous protocol of controlled quantum secure direct communication of Zhang et al.’s scheme, which was found insecure under two kinds of attacks, fake entangled particles attack and disentanglement attack. Then, by changing the party of the preparation of cluster states and using unitary operations, we present an improved protocol which can avoid these two kinds of attacks. Moreover, the protocol is proposed using the three-qubit partially entangled set of states. It is more efficient by only using three particles rather than four or even more to transmit one bit secret information. Given our using state is much easier to prepare for multiqubit states and our protocol needs less measurement resource, it makes this protocol more convenient from an applied point of view.     

基于局部分型面的电器外壳注塑模具设计

电器外壳制品形状结构复杂,有两处侧凸(孔)较难处理。通过对矩形孔内侧凸的结构分析,在其局部设计了分型面,避免了使用侧向抽芯机构;对侧孔部位设计了斜顶杆侧向抽芯机构,其结构简单、紧凑。模具采用一模二腔、平衡式布局,采用经扁推杆由制品内部进料的潜伏式浇口,S型流道。在NX 8.0中完成了模具结构设计。经实践证明,该模具结构合理,产品合格。     

Wear mechanism of a novel AlSiMgAl2O4Al2O3 composite used in the low vessel of an RH secondary refining furnace

A novel AlSiMgAl₂O₄Al₂O₃ composite brick was prepared and evaluated in the low vessel of an RH (the initials of Ruhrstahl and Hereaeus) secondary refining furnace; it was characterized by X-ray diffraction, scanning electron microscopy, and energy-dispersive spectroscopy. The results show that after use, the AlSiMgAl₂O₄Al₂O₃ composite has a functional gradient with an erosion zone–reinforced zone–original zone phase distribution, in which the phases in the erosion zone (0–1.8?cm) are a Mg-hercynite spinel solid solution, α-Al₂O₃, and minor amount of Al₃Fe₅O₁₂. Furthermore, the phases in the reinforced zone (1.8–5.0?cm) are γ-AlON, 21RSiAlON, SiC, Mg_0.388Al_2.408O₄, and α-Al₂O₃; i.e., the Al and Si in the composite are completely converted into non-oxide reinforced phases. Finally, the phases in the original zone (>5.0?cm) show no change. The reaction mechanism is as follows. During operation, a Mg-hercynite spinel solid solution is formed in the erosion zone due to a reaction between MgAl₂O₄ and FeO from a refinery operation. Therefore, the slag erosion of the material is improved. The Al and Si metals undergo active oxidation, and 21RSiAlON flakes are subsequently formed from the products of the metastable Al₂O(g), SiO(g), and N₂(g) in the ambient. The γ-AlON is formed by a carbothermal reduction nitridation of the α-Al₂O₃ and residual active carbon from the resin binder. The 21RSiAlON and γ-AlON reinforce the composite brick and improve its high temperature performance accordingly. Its service life is 110% that of the magnesia-chrome bricks used in the same period. The reaction model was also established.     

A Mobile Cloud-Based eHealth Scheme

Mobile cloud computing is an emerging field that is gaining popularity across borders at a rapid pace. Similarly, the field of health informatics is also considered as an extremely important field. This work observes the collaboration between these two fields to solve the traditional problem of extracting Electrocardiogram signals from trace reports and then performing analysis. The developed system has two front ends, the first dedicated for the user to perform the photographing of the trace report. Once the photographing is complete, mobile computing is used to extract the signal. Once the signal is extracted, it is uploaded into the server and further analysis is performed on the signal in the cloud. Once this is done, the second interface, intended for the use of the physician, can download and view the trace from the cloud. The data is securely held using a password-based authentication method. The system presented here is one of the first attempts at delivering the total solution, and after further upgrades, it will be possible to deploy the system in a commercial setting.     

Experimental study to assess the effect of carbon nanotube addition on the through-thickness electrical conductivity of CFRP laminates for aircraft applications

The present paper tests experimentally the through-thickness electrical conductivity of carbon fiber-reinforced polymer (CFRP) composites laminates for aircraft applications. Two types of samples were prepared: Type A samples with carbon nanotubes (CNTs) and Type B samples without CNTs. During the electrical experiments, electrical currents of several mA were injected through the specimens. Electrical resistance was monitored simultaneously in order to deduce the changes in the through-the-thickness electrical conductivity caused by the addition of CNTs. Improvement of electrical conduction by two orders of magnitude was achieved through the addition of 1 wt% carbon nanotubes as compared to classic CFRP without CNTs. For moisture saturated samples, the influence of moisture absorption on such measures was found to be negligible.