全文获取类型
收费全文 | 20697篇 |
免费 | 2842篇 |
国内免费 | 832篇 |
专业分类
电工技术 | 270篇 |
综合类 | 1039篇 |
化学工业 | 5315篇 |
金属工艺 | 134篇 |
机械仪表 | 454篇 |
建筑科学 | 136篇 |
矿业工程 | 29篇 |
能源动力 | 94篇 |
轻工业 | 12323篇 |
水利工程 | 36篇 |
石油天然气 | 75篇 |
武器工业 | 32篇 |
无线电 | 698篇 |
一般工业技术 | 983篇 |
冶金工业 | 80篇 |
原子能技术 | 85篇 |
自动化技术 | 2588篇 |
出版年
2024年 | 168篇 |
2023年 | 484篇 |
2022年 | 750篇 |
2021年 | 1566篇 |
2020年 | 852篇 |
2019年 | 972篇 |
2018年 | 785篇 |
2017年 | 883篇 |
2016年 | 829篇 |
2015年 | 966篇 |
2014年 | 1136篇 |
2013年 | 1279篇 |
2012年 | 1530篇 |
2011年 | 1543篇 |
2010年 | 1135篇 |
2009年 | 1040篇 |
2008年 | 1015篇 |
2007年 | 1241篇 |
2006年 | 1074篇 |
2005年 | 878篇 |
2004年 | 730篇 |
2003年 | 528篇 |
2002年 | 470篇 |
2001年 | 297篇 |
2000年 | 250篇 |
1999年 | 289篇 |
1998年 | 182篇 |
1997年 | 138篇 |
1996年 | 194篇 |
1995年 | 173篇 |
1994年 | 193篇 |
1993年 | 152篇 |
1992年 | 146篇 |
1991年 | 97篇 |
1990年 | 70篇 |
1989年 | 75篇 |
1988年 | 51篇 |
1987年 | 55篇 |
1986年 | 38篇 |
1985年 | 34篇 |
1984年 | 25篇 |
1983年 | 7篇 |
1982年 | 4篇 |
1981年 | 4篇 |
1980年 | 29篇 |
1979年 | 3篇 |
1978年 | 2篇 |
1976年 | 3篇 |
1974年 | 2篇 |
1951年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
61.
本实验尝试从马铃薯中提取蛋白质等成份,并适当添加姜汁,制成一种新型饮料,对饮料生产的工艺条件作了详细探讨,成功地解决了马铃薯褐变及沉淀等问题,确定了最佳配方及工艺。 相似文献
62.
Oxidation and gel-forming ability of chicken white (breast) and red (leg) muscle myofibrillar proteins during storage at 0°C were examined. Breast myofibril gels exhibited greater shear moduli than leg myofibril gels throughout 8 days storage. Shear moduli of both breast and leg gels in the intermediate temperature zone (45–55°C) decreased during storage, but at >55°C, they either increased or remained unchanged. Lipid oxidation was inhibited by washing myofibrils with antioxidants propyl gal-late, ascorbate, and tripolyphosphate. However, these antioxidants did not affect the content of protein carbonyls, and only slightly decreased the amine content during storage. Storage affected the kinetic process of myofibril gelation independently of antioxidant treatments. 相似文献
63.
Johann Vollmann Heinrich Grausgruber Helmut Wagentristl Heinrich Wohleser Pavel Michele 《Journal of the science of food and agriculture》2003,83(15):1581-1586
The presence of protease inhibitors in soybean prohibits the utilisation of the raw beans for food and feed. However, little information is available about environmental influences and the effects of nitrogen and sulphur supply on the antinutritional constituents of soybean. As these factors may influence protease inhibitors, soybean genotypes segregated according to the presence or absence of the Kunitz trypsin inhibitor have been evaluated for trypsin inhibitor activity (TIA) in field trials. TIA was affected significantly by environment (geographical location), fertilisation treatment and genotype. Environmental means of TIA were between 69.5 and 104.8 mg g?1. Nitrogen application, which caused an increase in seed protein content, resulted in a reduction in TIA by about 15% as compared with the control. Remarkably, simultaneous application of nitrogen and sulphur in the form of ammonium sulphate had a similar reductive effect on TIA to that of nitrogen application alone, although soybean protease inhibitors are rich in sulphur amino acids. Significant genetic variation in TIA was found both within the genotype class with the Kunitz inhibitor present as well as within the class lacking this inhibitor. The results suggest that TIA of soybean may be modified considerably by genetic improvement and appropriate agronomic management. Copyright © 2003 Society of Chemical Industry 相似文献
64.
大豆蛋白纤维的性能及产品开发 总被引:11,自引:4,他引:7
主要描述了大豆蛋白纤维的性能,介绍了大豆蛋白纤维的生产以及大豆蛋白纺织新产品的开发。 相似文献
65.
Preexposing rats to the context facilitates subsequent contextual fear conditioning. This effect depends on the hippocampus (J. W. Rudy, R. M. Barrientos, & R. C. O'Reilly, 2002). The authors report that inactivating the basolateral region of the amygdala (BLA) by injecting muscimol, a GABAA agonist, before or after preexposure reduced this effect. In contrast, bilateral injections of anisomycin, a protein synthesis inhibitor, into BLA did not impair the consolidation of the context memory. However, when injected after fear conditioning, anisomycin impaired consolidation of both contextual and auditory-cue fear conditioning. Results are consistent with 2 ideas about the amygdala's contribution to memory: (a) It modulates memory formation in other regions of the brain, and (b) it is a storage site for cue-shock associations. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
66.
67.
68.
乳腺癌中着丝粒蛋白CenpG的差异表达 总被引:4,自引:0,他引:4
用间接免疫荧光(IIF)验证了着丝粒蛋白CenpG的一种抗血清;通过对31例乳腺癌组织块及每一例病人的癌旁正常(非癌性)组织块蛋白提取物进行免疫印迹(Western blot)分析,发现多数情况下(71%)癌组织中着丝粒蛋白CenpG(95kD)过表达;在两例乳腺癌组织中还发现了一种能被CenpG抗血清识别的43kD蛋白质成分。结果表明,新近发现和命名的CenpG(或者还有其他相关成分)的差异表达可能与细胞恶性增殖有关。 相似文献
69.
ABSTRACT: Species identification of puffer species using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with Coomassie blue and Coomassie blue/silver double staining was performed. A comparison was also made among extracts of sarcoplasmic, myofibrillar, SDS-soluble, and urea-soluble proteins. Protein bands of lower molecular weight showed the species-specific characteristics. Double staining better discriminated the protein banding patterns of different puffer species than Coomassie blue staining. Species identification of all tested puffers could be achieved by judging from the SDS-PAGE patterns of the 4 protein extracts stained with both stains along with protein band densities. 相似文献
70.
The effects of induction conditions on production of a soluble antitumor sFv in Escherichia coli 总被引:2,自引:0,他引:2
Jaymie R.Sawyer; Schlom Jeffrey; Kashmiri S. V. S. 《Protein engineering, design & selection : PEDS》1994,7(11):1401-1406
CC49 is a second generation monoclonal antibody(mAb) with highaffinity to a pancarcinoma antigen, TAG-72. A single-chain Fv(sFv)ofCC49 may have a role in managing human carcinomas. Most reportedsFvs have been expressed as insoluble products that must besolubilized and renatured. Soluble sFv expression is advantageousas activity can be assayed directly from the periplasmic fraction.Also, gene-level immunoconjugates may not be amenable to refoldingprotocols. Using a vector that carries the tac promoter andomp A signal, we have examined the effects of four variableson the expression and accumulation of soluble CC49 sFv: (i)linker sequence joining VL and VH, (ii) isopropylthio-ß-D-galactosideconcentration for induction, (iii) temprature, and (iv) theaddition of nonmetabolizable sugars to the medium. We have beenable to demonstrate, using rapidly prepared periplasmic extracts,that the yield of soluble sFv improves by the addition of 0.4Msucrose to the medium and by inducing expression with a verylow concentration of IPTG (0.020.03 mM). Under theseinduction conditions periplasmic extracts demonstrate increasedexpression of the sFv, as shown by the larger amount of a 27kDa band on SDS-polyacrylamide gel, and an increased abilityto inhibit binding of the mAb CC49 to immobilized tumor extracts. 相似文献