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101.
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赵乐辉 《辽东学院学报(自然科学版)》2004,11(2):43-44
以前关于木霉菌的研究主要集中在其生物防治作用方面,对于木霉菌作为有害菌时如何选择杀菌剂进行化学防治以及木霉菌作为生防菌时如何协调生物防治和化学防治,这些问题研究得很少。为提高对病害的综合防治效果,本实验就三种常用于防治番茄灰霉病的杀菌剂,对五种木霉菌株的抑制能力进行了室内测定。 相似文献
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Epiphytic yeasts were isolated from different cultivars of apples and lemons and identified by a combination of PCR-RFLP of 5.8S rRNA region and sequencing of D1/D2 domain of the 26S rRNA gene. Among 69 isolates, Aureobasidium pullulans GE17 and Meyerozyma guilliermondii KL3 strains showed the greatest antagonistic activity against two significant apple and lemon postharvest pathogens, Penicillium expansum DSM62841 (blue mold) and Penicillium digitatum DSM2750 (green mold), after preliminary screening. Yeasts were applied as single and mixed cultures with two different cell concentrations of 106 and 108 cells/ml in the present study. It was determined that antagonistic activity of two yeast strains studied emerged with a combination of several mechanisms of action including competition for space and nutrients, production of volatile organic compounds (VOCs), secretion of extracellular lytic enzymes and inhibition of fungal spore germination. The highest inhibition of mycelial growth on P. expansum DSM62841 and P. digitatum DSM2750 (83.4% and 74.7%, respectively) was achieved by utilization of single culture of A. pullulans GE17. Otherwise, the application of mixed culture at the ratio of 108 cells/ml inhibited spore germination of both pathogens from 86% to 95%. Results of this study suggest that an increase in yeast cell concentrations positively affected their biocontrol activity against blue and green molds. According to the results, employing single culture of M. guilliermondii KL3 did not exhibit effective antagonistic activity against blue and green molds. However, utilization of A. pullulans GE17 alone and mixed culture showed succesfull controlling against both P. expansum DSM62841 and P. digitatum DSM2750. 相似文献
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Maribel Abadias Josep Usall Isabel Alegre Rosario Torres Inmaculada Viñas 《Journal of the science of food and agriculture》2009,89(9):1526-1533
BACKGROUND: Generally, acidic fruits and fruit juices are considered ‘safe’ from a microbiological point of view. However, some outbreaks of foodborne illnesses have been linked to the consumption of unpasteurised cider. The aim of this work was to study the survival of Escherichia coli in apple juice, wounds and flesh and on apple surfaces at different temperatures and to determine the effect of the fungal biocontrol agent Candida sake CPA‐1 against the colonisation of apple by E. coli. RESULTS: Trials were conducted with a mixture of five strains of E. coli isolated from apples. E. coli was unable to grow in apple juice at 5, 15 and 25 °C but survived. At 10 °C and above, E. coli thrived in fresh‐cut apple and wounds. At 5 °C it survived in apple wounds after 27 days of storage and after 21 days in fresh‐cut apples. When E. coli was inoculated in apple wounds together with the yeast antagonist C. sake, its growth was reduced by approximately 1 log cfu wound?1 at 25 °C. At 5 °C no effect of the biocontrol agent was observed. CONCLUSION: Despite the low pH of apple, a rapid increase in the bacterial population is possible if the temperature is not kept low enough. The biocontrol agent C. sake, developed to prevent fruit decay during storage, could also reduce E. coli growth in wounded apples at abusive temperatures. This would represent an additional benefit of using this biocontrol agent when applied to control postharvest diseases. Copyright © 2009 Society of Chemical Industry 相似文献
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The prevalence of bacteriophages infecting Staphylococcus aureus in dairy samples was assessed. Fourteen Staph. aureus strains were used in enrichment cultures of 75 dairy samples. All samples grew specific Staph. aureus bacteriophages. According to the host range, 8 different phages were isolated. Three of them, phages ΦH5, ΦG7, and ΦA72, were found in 89% of the samples; all the isolated phages were temperate. Phages ΦH5 and ΦA72 were used in preliminary bacterial challenge tests against Staph. aureus in milk. A phage mixture (1:1) was more effective than each single phage, most likely by preventing the survival of lysogenized cells. Phages inhibited Staph. aureus in UHT and pasteurized whole-fat milk. However, the phages were less active in semi-skimmed raw milk and little inhibition was achieved in whole, raw milk. Killing of Staph. aureus was observed at room temperature and at 37°C, but not at refrigeration temperature. 相似文献
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为研究裸脚菇0612-9活性物质对柑橘青、绿霉病的生防效果及其诱导抗性,以‘南丰’蜜桔为试材,采用质量浓度分别为0(对照)、0.25、0.55、5.50 mg/mL的裸脚菇0612-9发酵液粗提物(extract of fermentation broth from Gymnopus sp. 0612-9,GSFE)通过损伤接种的方式处理果实,随后分别接种青、绿霉病原菌孢子悬浮液,定期统计果实发病率,并取样测定柑橘果皮防御酶活力等指标的变化。结果表明:不同质量浓度GSFE处理较对照组均能显著降低柑橘青、绿霉病发病率及病情指数(P<0.05),其中GSFE质量浓度为5.50 mg/mL时的抑制效果最佳;同时,GSFE处理能够诱导‘南丰’蜜桔果皮中相关防御酶(苯丙氨酸解氨酶、超氧化物歧化酶、过氧化物酶)活力的提高,促进其抗氧化物质总酚和类黄酮的产生,并抑制脂氧合酶活力、减少丙二醛的积累、降低膜脂过氧化程度,从而提高‘南丰’蜜桔果实的抗病性。本研究结果可为设计开发GSFE类新型柑橘采后防腐保鲜剂提供思路。 相似文献
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采用室内筛选的11个生物防治菌株进行盆栽和田间小区防治番茄灰霉病试验结果表明,细菌B27,真菌F45和F120,放线菌A15和A111,具有较好的预防效果,防治效果均在60%以上,其中放线菌A15菌株,防治效果达70%以上。菌株组合真菌F20+F45和F20+F120,F45+F120和细菌B24+B28具有增效作用。其中F45+F120和B28+B24组合.防治效果分别为69.2%和70.2%。 相似文献