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91.
Inhibitory effect of Cr(Ⅵ) on activities of soil enzymes 总被引:1,自引:0,他引:1
To evaluate the influence of various Cr(VI) concentrations (0.05, 0.25, 0.50, 1.00 and 2.00 g/kg) on the activity of soil
enzymes, the activities of catalase, polyphenol oxidase, dehydrogenase, alkaline phosphatase in soils were investigated in
the incubation experiment with a period of 35 d. The results indicate that all the tested Cr(VI) concentrations significantly
inhibit dehydrogenase activity by over 70% after 35 d. The activity of alkaline phosphatase is slightly inhibited during the
whole experiment except for on the day 7. Cr(VI) has no obvious effect on the activity of catalase in soil. On the contrary,
Cr(VI) stimulates the activity of polyphenol oxidase. The results suggest that dehydrogenase activity can be used as an indicator
for assessing the severity of chromium pollution.
Foundation item: Projects(2006AA06Z374, 2007AA021304) supported by the National High-Tech Research and Development Program of China; Project(2008SK2007)
supported by the Key Program of Science and Technology of Hunan Province, China 相似文献
92.
金属离子对红球菌腈水合酶活力的影响 总被引:2,自引:0,他引:2
不同金属离子对红球菌(Rhodococcus sp.)TCCC 28001的生长及其腈水合酶的酶活有着不同程度的影响.菌株TCCC 28001产生的腈水合酶是钴型,且Co^2+对该腈水合酶诱导激活的最适浓度为10×10^-5mol/L.选择酵母粉中含有且含量波动较大的5种金属离子,考察了在添加10×10^-5 mol/L Co^2+诱导激活下,5种金属离子对酶活的影响.结果表明:Fe^2+,Mn^2+,Mo^6+,Cu^2+4种金属离子具有促进作用,Zn^2+产生轻微抑制作用.6×10^-5mol/L Fe^2+的促进效果显著,使菌株TCCC 28001的酶活从453U/mL增加到1941U/mL,提高了328%. 相似文献
93.
针对于Petersen图P9进行研究,利用两个Petersen图K3,3,1与中间边组成的图的方法来形成petersen图中的P9,本文得到了一种带有纽结分支的内在链图H(93),并证明了该定理. 相似文献
94.
针对反射型光纤传感器测量金免疫层析试条反应结果的工作原理和不足,在扫描检测的基本原理下,提出积分扫描的方法提取试条的检测线,并且分析该方法的可行性,给出了检测线提取的程序流程图,试验结果表明:该方法在检测线不倾斜的情况下,可以较精确地提取检测线。 相似文献
95.
采前水杨酸处理对哈密瓜果实防御酶的诱导 总被引:2,自引:0,他引:2
采用1.0、2.0、3.0 mmol/L水杨酸分别在"蜜农9号"哈密瓜开花前1周、幼果期(花后2周)、果实迅速膨大期(花后3周)和网纹形成期(花后4周)对植株进行4次喷洒.依次在幼果期、膨大期、网纹形成期和成熟期进行取样.分析不同浓度水杨酸处理后果实体内3种防御性酶活性的变化.结果表明:采前1.0、2.0、3.0 mmol/L水杨酸处理均能有效地提高哈密瓜果实中过氧化物酶(POD)、多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)活性,尤其以2.0 mmol/L水杨酸处理酶活性增加最为显著. 相似文献
96.
连通容器内气体爆炸过程的数值分析 总被引:1,自引:0,他引:1
为了研究连通容器内气体爆炸强度增加产生机理以及燃烧火焰与压力传播的基本规律,采用k-ε模型和EBU-Arrhen ius混合反应模型,从流体力学和化学反应动力学守恒方程出发,利用大型流体动力学软件F luent 6.1对连通容器内气体爆炸过程进行了数值分析,获得了气体爆炸过程中火焰和压力传播特性以及气体流动特性,模拟结果能够比较清晰地反映气体爆炸的整个过程。研究表明连通容器中气体爆炸过程中火焰始终是加速传播的,气体压缩和反流以及喷射火焰是连通容器气体爆炸强度增加的主要原因。 相似文献
97.
Akiko?Kawashima Toshihiro?NagaoEmail author Yomi?Watanabe Takashi?Kobayashi Ikuo?Ikeda Yoshio?Tominaga Yuji?Shimada 《Journal of the American Oil Chemists' Society》2004,81(11):1013-1020
TAG (MLM) with medium-chain FA (MCFA) at the 1,3-positions and long-chain FA (LCFA) at the 2-position, and TAG (LMM) with
LCFA at the 1(3)-position and MCFA at 2,3(1)-positions are a pair of TAG regioisomers. Large-scale preparation of the two
TAG regioisomers was attempted. A commercially available FFA mixture (FFA-CLA) containing 9-cis, 11-trans (9c, 11t)- and 10t,12c-CLA was selected as LCFA, and caprylic acid (C8FA) was selected as MCFA. The MLM isomer was synthesized by acidolysis of acyglycerols (AG) containing two CLA isomers with
C8FA: A mixture of AG-CLA/C8 FA (1∶10, mol/mol) and 4 wt% immobilized Rhizomucor miehei lipase was agitated at 30°C for 72 h. The ratio of MLM to total AG was 51.1 wt%. Meanwhile, LMM isomer was synthesized by
acidolysis of tricaprylin with FFA-CLA: A mixture of tricaprylin/FFA-CLA (1∶2, mol/mol) and 4 wt% immobilized R. miehei lipase was agitated at 30°C for 24 h. The ratio of LMM to total AG was 51.8 wt%. MLM and LMM were purified from 1,968 and
813 g reaction mixtures by stepwise short-path distillation, respectively. Consequently, MLM was purified to 92.3% with 49.1%
recovery, and LMM was purified to 93.2% with 52.3% recovery. Regiospecific analyses of MLM and LMM indicated that the 2-positions
of MLM and LMM were 95.1 mol% LCFA and 98.3 mol% C8 FA, respectively. The results showed that a process comprising lipase reaction and short-path distillation is effective for
large-scale preparation of high-purity regiospecific TAG isomers. 相似文献
98.
S. Yildirim R. G. Fuentes R. Evangelista Z. L. Nikoloy 《Journal of the American Oil Chemists' Society》2002,79(8):809-814
Two germ-separation methods, dry-milling and density separation by flotation, were evaluated for recovering recombinant β-glucuronidase
(rGUS) that accumulated primarily in the germ of transgenic corn. The dry-milling process consisted of (i) seed tempering,
(ii) degerming with a horizontal-drum degermer/dehuller, (iii) particle size fractionation with standard sieves, (iv) germ
and endosperm separation by roller milling and sifting, and (v) removal of hulls by aspiration. Sieves nos. 5, 6, and 7 retained
the majority of germ, and subfractions from these sieves were pooled as a germ-rich fraction. Mass balances showed that the
germ-rich fraction, which constituted 17% of the total dry-milled corn weight, contained 49% of rGUS activity and 64% of the
total recoverable oil. Germ fractionation by flotation was tested as a proof-of-concept method aimed at separating corn fractions
based on their difference in specific gravity (sp gr). The process consisted of impact-grinding of corn kernels followed by
density separation using 1.15 or 1.3 specific gravity sodium nitrate solution. The oil-containing germ fraction floated, whereas
the heavier endosperm fraction sedimented. The flotation method was simpler and resulted in higher enzyme recovery, that is,
the germ-rich fraction was 20% (w/w) of the initial corn weight, and accounted for 80% of rGUS activity and 77% of total oil.
The sodium nitrate solution did not have an adverse effect on the enzyme activity. 相似文献
99.
100.
Activity and stability of an alkaline lipase fromPenicillium cyclopium var.album (PG 37) were studied in surfactant and detergent solutions. Three anionic surfactants [Na salts of C12SO4 ? (sodium dodecyl sulfate), C12ØSO3/? (linear alkyl benzene sulfonate), and C11COO? (laurate)] and four homologous series of nonionic surfactants of C12–15 polyoxethylenated fatty alcohols (AEO3, AEO5, AEO7, and AEO9) were evaluated. At a concentration range of 3.2–40 μM, sodium dodecyl sulfate and laurate stimulated the activity of PG 37 lipase. At concentrations greater than 5.6 μM, linear alkylbenzene sulfonate inhibited PG 37 lipase activity. Nonionic surfactants, AEO5 and AEO7, in the concentration range of 0.25–20 mM, enhanced and stabilized the activity of PG 37 lipase. The presence of PG 37 lipase in detergent formulaton improved detergency ~20%. The mechanism of inhibition of the lipolytic activity of PG 37 lipase is proposed to be partly due to the formation of inactive (BR)n-E complex between the hydrophobic moiety of the surfactants and the surface of the lipase. Conversely, formation of a soluble (RB)n-E complex between the hydrophilic group of the surfactant and lipase may account for the increased lipolytic activity of PG 37 lipase. 相似文献