In this paper a simple preparative method for isolation and purification of ginkgolides A and B was developed,As starting material,a commercially available standardized ginkgo extract (EGb761,containing 24% flavonoid and 6% terpene trilactones) was used,After a pretreatment step,optimized by the uniform design method ,the concentrated intermediate extract with high content of GA and gb( 90%) was separated into the individual terpenes by preparative liquid chromatography eluted with petroleum ether-ethylacetate,Analysis of products was carried out by means of HPLC-ELSD(evaporative light -scattering detector),The results show that ginkgolides A and B are obtained in higher yield and better purity. 相似文献
n-3 Polyunsaturated fatty acids (n-3 PUFA) from the marine microalgaIsochrysis galbana were concentrated and purified by a two-step process—formation of urea inclusion compounds followed by preparative high-performance
liquid chromatography. These methods had been developed previously with fatty acids from cod liver oil. By the urea inclusion
compounds method, a mixture that contained 94% (w/w) stearidonic (SA), eicosapentaenoic (EPA), plus docosahexaenoic (DHA)
acids (4:1 urea/fatty acid ratio and 4°C crystallization final temperature) was obtained from cod liver oil fatty acids. Further
purification of SA, EPA, and DHA was achieved with reverse-phase C18 columns. These isolations were scaled up to a semi-preparative column. A PUFA concentrate was isolated fromI. galbana with methanol/water (80:20, w/w) or ethanol/water (70:30, w/w). With methanol/water, a 96% EPA fraction with 100% yield was
obtained, as well as a 94% pure DHA fraction with a 94% yield. With ethanol/water as the mobile phase, EPA and DHA fractions
obtained were 92% pure with yields of 84 and 88%, respectively. 相似文献
Dehydrofreezing process involves water partial removal before freezing. This treatment has been proposed in order to reduce the negative impacts of conventional or even accelerated freezing, especially on the textural quality of high water content fruits and vegetables. Indeed, in such cases, freezing and thawing processes result in severe damage of the integrity of product’s cell structure due to the formation of ice crystals. For this purpose, quince fruits (7?g H2O/g db) were subjected to convective air drying of 40?°C and 3m/s to reach different water content levels of 2, 1, and 0.3?g H2O/g db. Freezing profiles obtained at various freezing rates (V1, V2, and V3) for different water contents allowed the main freezing characteristics such as the Initial Freezing Temperature (IFT), the Practical Freezing time (PFt), and the Specific Freezing time (SFt) to be assessed. The impact of freezing rate was important on PFt and SFt, and more pronounced for high water contents (W between 7 and 2?g H2O/g db (dry basis)). Furthermore, IFT decreased sharply when initial sample water content decreased. Indeed, it started at ?0.8?°C for W?=?7g H2O/g db, while it reached a value of ?8.2?°C for samples of W?=?1g H2O/g db. Since convective air drying normally triggers shrinkage which causes a detrimental deformation of fruit structures, instant controlled pressure drop (DIC) treatment was used to improve the texture and enhance the whole dehydrofreezing performance and the final frozen-thawed product quality. Moreover, DIC implied a slight increase of PFt compared to untreated ones. On the other hand, quality attributes were estimated through the assessment of thawed water exudate (TWE g H2O/100?g db), color and texture (maximum puncture force as index of firmness): freezing rate and water content had great impacts on TWE. Hence, the lower the water content, the weaker the TWE. Furthermore, the TWE of the pre-dried quince (0.3?g H2O/g db) had higher value for DIC-textured samples than for the un-treated ones. Indeed, DIC-texturing leads to a well-controlled structure expansion of the cell wall. These textural changes resulted in more lixiviation of residual water. Consequently, water becomes more available, hence more releasable after thawing. Finally, the partial removal of water by air drying before freezing remarkably reduced the negative impact of freezing/thawing processes on final quince color. Decisively, the firmness of quince fruit increased with the decrease of water content level.
Abbreviations: DMC: Dry Matter Concentration (%); DIC: Instant controlled pressure drop; W: Water content dry basis (g H2O/g db); IFT: Initial Freezing Temperature (°C); PFt: Practical Freezing time (min); SFt: Specific Freezing time (min); TWE: Thawed Water Exudate (g H2O/100?g db); L, a, and b: Color coordinates; (L): The degrees of lightness; (a) and (–a): The redness (a) or greenness (?a), respectively; (b) and (?b): The yellowness (b) or blueness (?b), respectively; ΔE*ab: Total color difference; L0, a0, and b0: Color coordinates of fresh or dried quince samples; SD: Standard Deviation; ANOVA: Analysis of variances; LSD: Least Significant Differences; cp: Specific Heat of the product depending on composition (dry material and water content)(KJ/kg K); cpd: Specific Heat of the dry material (KJ/kg K); cpW: Specific Heat of water (KJ/kg K); V1: Freezing rate without insulation; V2: Freezing rate with a food stretch film insulation with thickness e2?=?3?mm and thermal conductivity λ2?=?0.17 W/m K; V3: Freezing rate with a versatile flexible insulation (Armacell) with thickness e3?=?13mm and weak thermal conductivity λ3?=?0.036 W/m K; vd: Volume of dry material of quince sample (mm3); vH2O: Volume of quince sample water (mm3); vt: Total volume of quince sample (mm3); e0: Quince sample thickness (mm); e2: Insulation thickness in the case V2; = 3?mm; ; e3: Insulation thickness in the case V3; = 13?mm; ; λ0: Quince sample conductivity (W/m K); λ2: Insulation conductivity in the case V2;?=?0.17 W/m K; ; λ3: Insulation conductivity in the case V3;?=?0.036 W/m K; λd: Conductivity of quince sample dry material (W/m K); λH2O: Conductivity of water (W/m K); λequiv: Equivalent conductivity of quince sample versus water content (W/m K); mi and mf: Weights of the frozen and thawed samples, respectively 相似文献
