Estimation of bacterial production through the coupling of (methyl 3H) Thymidine incorporation and several parameters over a Microcystis aeruginosa bloom in a hypereutrophic reservoir

The coupling of the changes in bacterial quantitative and metabolic aspects during Microcystis aeruginosa bloom conditions together with several environmental parameters was studied in the hypereutrophic Villerest reservoir, France. Bacterial abundance varied from 5.20 to 21.28 × 10⁶ bacteria-mL^?1, while bacterial biomass ranged between 75 and 507 μg C mL^?1. These results confirmed the highly eutrophic status of the Villerest reservoir. The relative quantitative importance of attached bacteria increased as Microcystis proliferated. Methyl-³H incorporation and D-(U-¹⁴C) glucose uptake varied from 8.99 to 60.57 × 10⁶ cells-mL^?1, and 0.016 to 1.587 μg CL^?1 h^?1, respectively. Correlations between several abiotic and biotic parameters showed that phytoplankton regulated bacterial growth by releasing organic carbon which is directly uptaken by heterotrophic bacteria. In the hypolimnion, the sedimentation of decaying algae represented a substantial substrate for bacterial growth.     

Distributed memory systems for simulating artificial neural networks

In executing tasks involving intelligent information processing, the human brain performs better than the digital computer. The human brain derives its power from a large number [O(10¹¹)] of neurons which are interconnected by a dense interconnection network [O(10⁵) connections per neuron]. Artificial neural network (ANN) paradigms adopt the structure of the brain to try to emulate the intelligent information processing methods of the brain. ANN techniques are being employed to solve problems in areas such as pattern recognition, and robotic processing. Simulation of ANNs involves implementation of large number of neurons and a massive interconnection network. In this paper, we discuss various simulation models of ANNs and their implementation on distributed memory systems. Our investigations reveal that communication-efficient networks of distributed memory systems perform better than other topologies in implementing ANNs.     

免棱镜全站仪检测建筑缺陷

本文介绍免棱镜全站仪及免棱镜测距原理,结合一个实例阐述工程应用——检测建筑缺陷。最后对其它应用和需要注意的问题作了简要说明。     

发酵香肠发酵过程中菌落总数数学模型的建立

用SAS9．0中的响应曲面试验设计，研究了盐浓度、水分活度、发酵温度、发酵时间和糖浓度等环境因子对发酵香肠发酵过程中细菌总数变化的影响，建立科学的数学模型，此数学模型能够表达发酵香肠发酵过程中细菌总数的变化趋势。用建立的数学模型对发酵过程中的微生物进行了预测，结果可靠。细菌总数与实验测定数据进行比较，平均误差范围是-10．20％～8．43％。此数学模型为发酵香肠发酵过程的控制，提高发酵肉制品的安全性，缩短生产周期提供了依据，也是微生物预报的重要基础。     

测量机器人GeoCOM接口技术的开发与应用

介绍了测量机器人(TCA2003全站仪)的一些强大功能,分析了其自带的GeoCOM(地理数据通信)接口技术的高级模式,在此基础上利用visual Basic 6.0语言和Access数据库,通过GeoCOM接口对测量机器人进行了二次开发,并应用于某水利枢纽加密控制网的自动观测.最后,通过平差计算得出了一些参考性结论.     

Business programmes and information systems methodologies

Abstract. An Information Systems Methodology is usually presented as a sequential set of tasks — planning, analysis, design and construction — resulting in a new business application. This classical 'forward-engineering' approach must be adapted to changing business needs — business process redesign, quality management, process and systems templates, advanced technologies, application packages, the reuse of existing information systems resources, and rapid delivery cycles. Using the most general concept of a Business Programme, this paper describes a methodological approach incorporating these modern themes that is qualitatively and quantitatively different from the classical model. It provides a framework for the integration of otherwise independent methodologies, and shows how an entire programme can be formulated, planned and executed. An example is provided, and the approach is justified.     

DETERMINATION OF TOTAL NITROGEN IN BARLEY AND MALT BY COMBUSTION METHOD—COLLABORATIVE TRIAL

A combustion method, relying on the Dumas principle, for the determination of total nitrogen in barley and malt, has been collaboratively tested by the Analysis Committee of the European Brewery Convention. Repeatability, r₉₅, and reproducibility, R₉₅, values were 0.063 and 0.116% of dry matter, respectively, for samples with nitrogen contents in the range 1.23 to 1.86% N of dry matter. There was no significant difference between these values for barley and malt. The Analysis Committee approved the adoption of the combustion method for inclusion in Analytica EBC as an alternative method.     

The effects of induction conditions on production of a soluble antitumor sFv in Escherichia coli

CC49 is a second generation monoclonal antibody(mAb) with highaffinity to a pancarcinoma antigen, TAG-72. A single-chain Fv(sFv)ofCC49 may have a role in managing human carcinomas. Most reportedsFvs have been expressed as insoluble products that must besolubilized and renatured. Soluble sFv expression is advantageousas activity can be assayed directly from the periplasmic fraction.Also, gene-level immunoconjugates may not be amenable to refoldingprotocols. Using a vector that carries the tac promoter andomp A signal, we have examined the effects of four variableson the expression and accumulation of soluble CC49 sFv: (i)linker sequence joining VL and VH, (ii) isopropylthio-ß-D-galactosideconcentration for induction, (iii) temprature, and (iv) theaddition of nonmetabolizable sugars to the medium. We have beenable to demonstrate, using rapidly prepared periplasmic extracts,that the yield of soluble sFv improves by the addition of 0.4Msucrose to the medium and by inducing expression with a verylow concentration of IPTG (0.02–0.03 mM). Under theseinduction conditions periplasmic extracts demonstrate increasedexpression of the sFv, as shown by the larger amount of a 27kDa band on SDS-polyacrylamide gel, and an increased abilityto inhibit binding of the mAb CC49 to immobilized tumor extracts.     

MPC系统模块结构中的流场研究

本研究中,应用Simple数值计算方法开发了求解MPC系统模块结构中二维流场分布的计算软件。为了验证计算结果的精确性,用多普勒测速仪实测了用有机玻璃制成的MPC模块二维模型的流场,模拟计算结果与实测值对比取得了较好的一致性。四种不同流出角的MPC模块平均总压损失计算结果表明,已开发的软件可作为设计低流阻MPC模块的良好工具。     

New method for measuring an optical property of a sample treated by FWA

In this article, a new method for measuring a total spectral radiance factor of a FWA‐treated sample illuminated by a specific standard illuminant is introduced. The method replaces an unstable real fluorescent standard by a bi‐spectral luminescent radiance factor data, which works as a virtual fluorescent standard (VFS) by knowing spectral intensity distributions of illuminations applied to the sample. The method utilizes two illuminations I₁ and I₂ whose relative spectral intensity distributions are different from each other and synthesizes a virtual illumination presenting the identical fluorescent spectral radiance factor to that presented by the standard illuminant with the VFS of the specific bi‐spectral luminescent radiance factor by linearly combining I₁ and I₂ with the suitable weighting factors. The applicability of the method is examined in principle by comparing ISO brightness and CIE whiteness index of fluorescent standard paper as a test sample obtained by this new method to the assigned values. © 2007 Wiley Periodicals, Inc. Col Res Appl, 32, 195–200, 2007