全文获取类型
收费全文 | 6852篇 |
免费 | 775篇 |
国内免费 | 311篇 |
专业分类
电工技术 | 40篇 |
综合类 | 388篇 |
化学工业 | 2424篇 |
金属工艺 | 141篇 |
机械仪表 | 158篇 |
建筑科学 | 380篇 |
矿业工程 | 29篇 |
能源动力 | 41篇 |
轻工业 | 2556篇 |
水利工程 | 41篇 |
石油天然气 | 81篇 |
武器工业 | 10篇 |
无线电 | 229篇 |
一般工业技术 | 594篇 |
冶金工业 | 116篇 |
原子能技术 | 67篇 |
自动化技术 | 643篇 |
出版年
2024年 | 36篇 |
2023年 | 148篇 |
2022年 | 320篇 |
2021年 | 733篇 |
2020年 | 266篇 |
2019年 | 250篇 |
2018年 | 239篇 |
2017年 | 221篇 |
2016年 | 271篇 |
2015年 | 357篇 |
2014年 | 401篇 |
2013年 | 481篇 |
2012年 | 464篇 |
2011年 | 480篇 |
2010年 | 342篇 |
2009年 | 361篇 |
2008年 | 324篇 |
2007年 | 356篇 |
2006年 | 294篇 |
2005年 | 310篇 |
2004年 | 222篇 |
2003年 | 188篇 |
2002年 | 124篇 |
2001年 | 90篇 |
2000年 | 76篇 |
1999年 | 71篇 |
1998年 | 70篇 |
1997年 | 52篇 |
1996年 | 57篇 |
1995年 | 56篇 |
1994年 | 39篇 |
1993年 | 24篇 |
1992年 | 29篇 |
1991年 | 31篇 |
1990年 | 24篇 |
1989年 | 18篇 |
1988年 | 8篇 |
1987年 | 29篇 |
1986年 | 9篇 |
1985年 | 23篇 |
1984年 | 20篇 |
1983年 | 8篇 |
1982年 | 4篇 |
1981年 | 2篇 |
1980年 | 5篇 |
1979年 | 1篇 |
1976年 | 1篇 |
1964年 | 1篇 |
1960年 | 1篇 |
1951年 | 1篇 |
排序方式: 共有7938条查询结果,搜索用时 203 毫秒
21.
聚α烯烃基础油的生物降解性和毒性 总被引:4,自引:0,他引:4
低粘度聚α烯烃(PAO)基础油,在CEC-L-33-T-82试验程序的条件下是容易生物降解的。PAO基础油对哺乳动物是无毒和无刺激性的,在海洋微生物荧光细菌的Microtox试验中,对含有49000μg/g浓度的PAO水溶性馏分,没有发现明显的影响。 相似文献
22.
Genetically modified starter and protective cultures 总被引:1,自引:0,他引:1
Modern approaches towards starter and protective culture improvement rely on advances in molecular biology. For most microorganisms used for food production, gene technological methods have been well developed. By recombinant DNA technology, ‘tailor-made’ starter and protective cultures may be constructed so as to combine technically desirable features. A single strain which normally would fail to accomplish a given ‘wtask’ may now be improved so as to meet a set of requirements necessary for a specific production or preservation process (e.g. wholesomeness, no off-flavour production, overproduction of bacteriocins or particular enzymes). In addition, undesirable properties (e.g. mycotoxin or antibiotic production by cheese moulds) may be eliminated by techniques such as ‘gene disruption’. 相似文献
23.
We address the problems of noise and huge data sizes in microarray images. First, we propose a mixture model for describing the statistical and structural properties of microarray images. Then, based on the microarray image model, we present methods for denoising and for compressing microarray images. The denoising method is based on a variant of the translation-invariant wavelet transform. The compression method introduces the notion of approximate contexts (rather than traditional exact contexts) in modeling the symbol probabilities in a microarray image. This inexact context modeling approach is important in dealing with the noisy nature of microarray images. Using the proposed denoising and compression methods, we describe a near-lossless compression scheme suitable for microarray images. Results on both denoising and compression are included, which show the performance of the proposed methods. Further experiments using the results of the proposed near-lossless compression scheme in gene clustering using cell-cycle microarray data for S. cerevisiae showed a general improvement in the clustering performance, when compared with using the original data. This provides an indirect validation of the effectiveness of the proposed denoising method. 相似文献
24.
国内外微生物采油技术综述 总被引:24,自引:4,他引:20
生物技术特别是微生物采油技术,已经引起了石油工程技术人员的空前关注,目前在国内外开展的微生物采油先导性矿场试验已初见成效,较为典型的当数美国和俄罗斯,我国的吉林、胜利等油田也进行了矿场试验,增油效果令人鼓舞。同时,随着科学技术的发展,一些先进的微生物学研究工具已经进入微生物采油技术的研究领域,如分子生物学、示踪剂及可视化技术等已经成为微生物采油机理研究的重要工具。相关的石油烃降解理论以及建立数学模型等也取得了系列成果。微生物采油的应用也从过去的单井处理逐渐向整个区块或油田发展,并见到了明显效果。 相似文献
25.
Salinger Walter L.; Ladrow Pamela; Wheeler Catherine 《Canadian Metallurgical Quarterly》2003,117(6):1257
Reeler (rl/rl) and reeler/wild-type (+/rl) mice synthesize Reln at subnormal rates, as do patients with schizophrenia, bipolar disorder, and autism, thereby forming the basis for a Reln hypothesis for vulnerability to these psychopathologies and justifying attention to the behavioral phenotypes of Reln-deficient mice. Tests of gait, emotionality, social aggression, spatial working memory, novel-object detection, fear conditioning, and sensorimotor reflex modulation revealed the behavioral phenotype of rl/rl, but not +/rl, mice to be different from that of wild-type (+/+) mice. These results reveal no effect of Reln gene dosage and provide significant challenges to both the Reln and the neurodevelopmental hypotheses of the etiology of major psychopathologies. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
26.
目的制备新生牛肝活性肽并评价其安全性。方法采用膜法分离制备新生牛肝活性肽。将3批制品于37~40℃,75%相对湿度条件下存放3个月,以多肽含量为指标观察其稳定性,并进行急性毒性试验、小鼠骨髓细胞微核试验、Ames试验、小鼠精子畸形试验和大鼠喂养试验。结果制备的3批新生牛肝活性肽存放3个月后,多肽含量无明显下降,质量稳定。小鼠和大鼠经口灌人大于20.0g/kg体重的新生牛肝活性肽,均无急性毒性。3种致突变试验均未显示出致突变性,大鼠喂养试验各项指标均未见明显毒性。结论新生牛肝活性肽未表现出明显毒性。 相似文献
27.
Yeast mutants with increased bacterial transposon Tn5 excision 总被引:7,自引:0,他引:7
D A Gordenin Y Y Proscyavichus A L Malkova M V Trofimova A Peterzen 《Yeast (Chichester, England)》1991,7(1):37-50
Five complementing recessive mutations that exhibit increased bacterial transposon Tn5 precise excision in yeast Saccharomyces cerevisiae were obtained by ethylmethanesulfonate treatment. One of these mutations (tex1) was submitted to extensive genetic analysis. tex1 is a recessive temperature-sensitive mutation resulting in a 20-100-fold increase in Tn5 excision. It also has increased frequencies of ochre mutation reversion, of forward mutation to canavanine resistance, and loss of chromosome III or its right arm. The possible mechanism of tex1 effects is discussed. 相似文献
28.
筛查基因突变的新技术——循环双脱氧指纹图谱法及其应用 总被引:2,自引:0,他引:2
建立了适用于大批量标本基因突变筛查的双脱氧指纹图谱法即ddF。该技术民测定DNA序列的双脱氧末端终止法及SSCP二者长处于一身,即只作一个双脱氧末端终止反应,然后将反应产物在与SSCP相同的电泳条件下进行聚丙烯酰胺凝胶电泳,这样有突变的样品不会漏检,阳性检出率高达100%。 相似文献
29.
聚乙二醇修饰的蓖麻毒蛋白A链的免疫原性和在小鼠体内急性毒性研究 总被引:2,自引:0,他引:2
目的研究单甲氧聚乙二醇(mPEG)与蓖麻毒蛋白A链(RA)偶联后RA免疫原性和毒性的改变。方法从天然蓖麻籽中提取纯化的RA,经与含活性马来酰亚胺基团的mPEG反应偶联,得到纯化的mPEG偶联RA。用蛋白质印迹方法比较RA修饰前后的免疫原性,通过测定半数致死量(LD50)比较RA修饰前后的毒性。结果mPEG偶联RA后免疫原性和毒性均明显下降。偶联前RA链的LD50为2.37 mg.kg-1,经mPEG修饰后RA的LD50为6.15mg.kg-1。结论聚乙二醇能遮蔽RA,降低其免疫原性和毒性。 相似文献
30.
The soil microbial biomass quotient (expressed as a percentage of the total soil organic carbon) and the specific rate of carbon-dioxide production by soil microbes (respiration quotient) are often used as indicators of stress on soil microbial populations. A low biomass quotient or a high respiration quotient is considered to be an indication of stress from, for example, toxicity from metals in sewage sludge applied to soils. These metabolic quotients are affected by a wide variety of other factors such as the biodegradability of soil organic-carbon amendments, plant inputs of organic carbon into soils, natural variations in microbial population sizes with depth, and in the rhizosphere of plants. These variations could be sufficiently large to make interpretation of changes in biomass quotient and respiration quotient, as a response to stress, problematical. 相似文献