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排序方式: 共有676条查询结果,搜索用时 625 毫秒
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Quantification of Protein‐Induced Membrane Remodeling Kinetics In Vitro with Lipid Multilayer Gratings 下载免费PDF全文
Troy W. Lowry Plengchart Prommapan Aubrey Kusi‐Appiah Nicholas Vafai Ewa A. Bienkiewicz David H. Van Winkle Scott M. Stagg Steven Lenhert 《Small (Weinheim an der Bergstrasse, Germany)》2016,12(4):506-515
The dynamic self‐organization of lipids in biological systems is a highly regulated process that enables the compartmentalization of living systems at micro‐ and nanoscopic scales. Consequently, quantitative methods for assaying the kinetics of supramolecular remodeling such as vesicle formation from planar lipid bilayers or multilayers are needed to understand cellular self‐organization. Here, a new nanotechnology‐based method for quantitative measurements of lipid–protein interactions is presented and its suitability for quantifying the membrane binding, inflation, and budding activity of the membrane‐remodeling protein Sar1 is demonstrated. Lipid multilayer gratings are printed onto surfaces using nanointaglio and exposed to Sar1, resulting in the inflation of lipid multilayers into unilamellar structures, which can be observed in a label‐free manner by monitoring the diffracted light. Local variations in lipid multilayer volume on the surface is used to vary substrate availability in a microarray format. A quantitative model is developed that allows quantification of binding affinity (K D) and kinetics (kon and koff). Importantly, this assay is uniquely capable of quantifying membrane remodeling. Upon Sar1‐induced inflation of single bilayers from surface supported multilayers, the semicylindrical grating lines are observed to remodel into semispherical buds when a critical radius of curvature is reached. 相似文献
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The use of moiré fringe techniques in microfabrication equipment is reviewed with special emphasis on mask-to-wafer alignment. The alignment strategies and associated detection methods of a number of commercial wafer steppers are described and their limitations are discussed. Potential improvements to be gained by holographic detection of grating targets are pointed out and future applications in a dynamic exposure system are proposed. 相似文献
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0~cnoxTIsacwmpsondb},bakeralSciencefondationOfHeilonaiiangPro'ince.Theopto-mechanicalscarmingmayhefoebottleneckinlasercoherenticingsystemsthatpreventframeratesandfieldsofviewfromincreasing,thereforeiteannotsatisfywiththereqUirementSofhunting,backing,gUidingandcontIDllingandsoon.Inaddihon,thoses}'stemsoftensufferfromaconsidelablelossofimageqUalityduetOspecklenoise.ThisspecklenoiseiscausedbytheconstrUctiveanddestIUctiveinterferenceofwavefrontSreflectedfromaroughaptandbythelargespatialcoher… 相似文献
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L. A. Ferreira E. V. Diatzikis P. J. Moreira J. L. Santos F. Farahi 《Optical Fiber Technology》2000,6(4):1132
The interrogation of fiber Bragg grating sensors with multimode laser diodes is theoretically and experimentally analyzed using two different approaches: serrodyne and dithering techniques. It is also demonstrated that by using several laser modes it is possible to enhance the measurement range without compromising sensitivity. 相似文献
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O. Martínez-Matos J. A. Rodrigo M. L. Calvo V. Hevia-Martín P. Cheben 《Optical Memory & Neural Networks》2009,18(1):21-24
The properties of a photopolymerizable glass modified with high refractive index species incorporated at molecular level,
including a wide range of spatial frequencies and the recording kinetics, are analyzed. Implementation of emerging applications
is proposed.
The article is published in the original. 相似文献
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高质量莫尔条纹信号的提取 总被引:9,自引:6,他引:3
本文分析了影响光栅信号质量的主要因素,指出了获得高质量光栅信号的途径,提出了一般设计方法及各参数的选择。文中给出的实验测试结果成功地用于JC-1型精密测角仪中。 相似文献
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为了实现机载小型化FSM的闭环控制,设计了一款小体积、高精度、高环境适应性的微位移测量传感器。首先根据位移传感器的技术原理和机载FSM的应用需求,提出了微位移测量传感器的主要设计指标。然后,分别对微位移测量传感器的发光元件、密珠滑轨、测量光栅和接收元件等进行了详细设计与选择。其中,高精度密珠滑轨的采用不仅减小了测量过程中的附加阻力,而且使光栅副的运动更加精准;光栅副通光窗口的引入、独特零件编码的设计、裂相法信号提取方式的采用,保证了微位移测量传感器的高低温性能。最后,针对微位移测量传感器在机载小型化FSM上的应用效果进行了理论分析与实验检测。结果显示:机载小型化FSM的方位指向误差不超过3.4″,俯仰指向误差不超过3.8″,证明了专用型微位移测量传感器高精度、高分辨力和高响应速度的技术特点,满足机载小型化FSM的应用需求。 相似文献