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101.
为了研究遗传密码子对表达调控的影响,利用PCR重叠延伸法,对萝卜抗真菌蛋白Rs-AFP2基因编码序列区的部分核苷酸进行沉默突变,构建突变体Rs-AFPm.序列分析表明,PCR产物全长240bp,有一个阅读框,编码的蛋白由29个氨基酸的信号肽和51个氨基酸的抗真菌蛋白组成.突变体与突变前的Rs-AFP2基因相比,在编码区第3号氨基酸Lys相差一个碱基(TTG→TTA),第5号氨基酸Gln相差一个碱基(CAG→CAA),第6号稀有密码子Arg相差两个碱基(CAG→CGA).重新合成引物,将切除信号肽的Rs-AFP2基因和Rs-AFPm基因与原核表达载体pET-21b(+)分别重组到大肠杆菌BL21菌株.IPTG诱导后,二者均得到了表达.软件分析显示,突变前pETAFPo表达产物占全菌蛋白的3%,突变后pETAFPm的表达产物占全菌蛋白含量的8%;表达蛋白主要以包涵体的形式存在,包涵体经超声波破碎后,蛋白质复性,抑菌结果表明,pETAFPm表达产物的抑菌半径大于pETAFP2表达产物的抑菌半径.这些都说明改造后的Rs-AFPm基因与Rs-AFP2基因相比,已有效地提高表达量. 相似文献
102.
The Two-Dimensional Clifford-Fourier Transform 总被引:1,自引:0,他引:1
Fred Brackx Nele De Schepper Frank Sommen 《Journal of Mathematical Imaging and Vision》2006,26(1-2):5-18
Recently several generalizations to higher dimension of the Fourier transform using Clifford algebra have been introduced,
including the Clifford-Fourier transform by the authors, defined as an operator exponential with a Clifford algebra-valued
kernel.
In this paper an overview is given of all these generalizations and an in depth study of the two-dimensional Clifford-Fourier
transform of the authors is presented. In this special two-dimensional case a closed form for the integral kernel may be obtained,
leading to further properties, both in the L
1 and in the L
2 context. Furthermore, based on this Clifford-Fourier transform Clifford-Gabor filters are introduced.
AMS subject classification numbers: 42B10, 30G35
Fred Brackx received a diploma degree in mathematics from Ghent University, Belgium, in 1970 and a Ph.D. degree in mathematics from the
same university in 1973. Since 1984 he is professor for mathematical analysis at Ghent University and currently he is leading
the Clifford Research Group. His main interests are function theory and functional analysis for functions with values in quaternion
and Clifford algebras. The research covers Clifford distributions, generalized Fourier, Radon and Hilbert transforms, orthogonal
polynomials and multi-dimensional wavelets.
Nele De Schepper received a diploma degree in mathematics from Ghent University, Belgium, in 2001. Since then she holds an assistantship at
the Department of Mathematical Analysis of Ghent University and is a member of the Clifford Research Group. Her main interests
are function theory and functional analysis for functions with values in Clifford algebras. The research covers generalized
Fourier transforms, orthogonal polynomials and multi-dimensional wavelets.
Frank Sommen received a diploma degree in mathematics from Ghent University, Belgium, in 1978, a Ph.D. degree in mathematics from the
same university in 1980, and a habilitation degree in mathematical analysis in 1984. From 1978 until 1999 he was at the National
Fund for Scientific Research (Flanders). Since 2000 he holds a Research professorship at Ghent University. His main interests
are function theory and functional analysis for functions with values in quaternion and Clifford algebras. The research covers
Clifford distributions, generalized Fourier, Radon and Hilbert transforms, orthogonal polynomials and multi-dimensional wavelets,
algebraic analysis, hyperfunctions and radial algebra. 相似文献
103.
电力系统谐波检测的现状与发展 总被引:7,自引:0,他引:7
准确、实时地对电力系统谐波进行检测有着重要的意义。本文根据电力系统谐波测量的基本方法,对近年来电力系统谐波检测的新方法进行了分析和评述。最后对电力系统的谐波测量进行了总结并提出了看法。 相似文献
104.
基于扩展分形和CFAR特征融合的SAR图像目标识别 总被引:3,自引:0,他引:3
研究了多信息融合技术在SAR图像目标识别中的应用。将扩展分形特征(Extended Fractal)与双参数恒虚警特征(Double Parameter CFAR)形成的多信息进行融合处理。运用Dempster-Shafer证据理论,在决策层对SAR图像中的像素进行识别分类。实验结果表明通过融合对像素分类的准确性明显好于单特征的检测结果,减少了虚警概率,提高了系统的识别能力。 相似文献
105.
提出一种视频点播系统传送数据的结构,结合单播与群播两种数据传送的方法,通过动态改变群播与单播的频道分配比例,让大部分的使用者无需等待即可开始观看影片,进而减少整体使用者的等待时间. 相似文献
106.
CMP系统技术与市场 总被引:3,自引:1,他引:2
葛劢冲 《电子工业专用设备》2003,32(1):17-24
概述了CMP系统技术的发展历史、发展趋势以及在IC生产中的重要性,介绍了国外CMP设备主要制造厂家的设备型号和性能及CMP设备市场分布和需求,阐述了CMP系统技术的基础研究、关键技术和国内研究概况。 相似文献
107.
108.
A stochastic model for local disturbances, particularly for a temporal harmonic with random modulations in amplitude and/or phase, is proposed in this paper. Results for the second moment responses of a linear single-degree-of-freedom system to this type of stochastic loading demonstrate a significant change in response characteristics due to a small uncertainty. A local phenomenon may last much longer and resonance may be smeared to a broad range. Integrated with wavelet transform, the proposed approach may be used to model a random process with non-stationary frequency content. Especially, it can be effectively used for Monte Carlo simulation to generate large size of samples that have similar characteristics in time and frequency domains as a pre-selected mother sample has. The technique has a great potential for the case where uncertainty study is warranted but the available samples are limited. 相似文献
109.
Polymer networks in which poly(propylene imine) dendrimers (Astramol?) are connected to each other by linear polytetrahydrofuran (polyTHF) segments, were prepared by two methods. The first method was a one‐step procedure in which bifunctionally living polyTHF, obtained by initiation of the THF polymerization with trifluoromethane sulfonic anhydride (triflic anhydride), was reacted with an amino‐dendrimer. This reaction was very fast but did not allow formation of the end products. The second method was a two‐step procedure. In a first step, living polyTHF, prepared with acryloyloxybutyl triflate as initiator, was grafted on an amino‐dendrimer, to form a star‐like, acrylate‐terminated polyTHF multi‐macromonomer with the dendrimer as core. In a second step, networks were obtained by Michael addition between the acrylate end‐groups and unreacted amino‐groups of the dendrimer. This cross‐linking reaction occurred spontaneously upon heating of the solution of the multi‐macromonomer with gelation times varying from a few minutes to a few hours, depending on the temperature and the composition of the prepolymers. With this method it was possible to prepare networks in the form of coatings or films. © 2003 Society of Chemical Industry 相似文献
110.