Effects of Adenine Methylation on the Structure and Thermodynamic Stability of a DNA Minidumbbell

DNA methylation is a prevalent regulatory modification in prokaryotes and eukaryotes. N¹-methyladenine (m¹A) and N⁶-methyladenine (m⁶A) have been found to be capable of altering DNA structures via disturbing Watson–Crick base pairing. However, little has been known about their influences on non-B DNA structures, which are associated with genetic instabilities. In this work, we investigated the effects of m¹A and m⁶A on both the structure and thermodynamic stability of a newly reported DNA minidumbbell formed by two TTTA tetranucleotide repeats. As revealed by the results of nuclear magnetic resonance spectroscopic studies, both m¹A and m⁶A favored the formation of a T·m¹A and T·m⁶A Hoogsteen base pair, respectively. More intriguingly, the m¹A and m⁶A modifications brought about stabilization and destabilization effects on the DNA minidumbbell, respectively. This work provides new biophysical insights into the effects of adenine methylation on the structure and thermodynamic stability of DNA.     

Role of Chiral Configuration in the Photoinduced Interaction of D- and L-Tryptophan with Optical Isomers of Ketoprofen in Linked Systems

The study of the L- and D-amino acid properties in proteins and peptides has attracted considerable attention in recent years, as the replacement of even one L-amino acid by its D-analogue due to aging of the body is resulted in a number of pathological conditions, including Alzheimer’s and Parkinson’s diseases. A recent trend is using short model systems to study the peculiarities of proteins with D-amino acids. In this report, the comparison of the excited states quenching of L- and D-tryptophan (Trp) in a model donor–acceptor dyad with (R)- and (S)-ketoprofen (KP-Trp) was carried out by photochemically induced dynamic nuclear polarization (CIDNP) and fluorescence spectroscopy. Quenching of the Trp excited states, which occurs via two mechanisms: prevailing resonance energy transfer (RET) and electron transfer (ET), indeed demonstrates some peculiarities for all three studied configurations of the dyad: (R,S)-, (S,R)-, and (S,S)-. Thus, the ET efficiency is identical for (S,R)- and (R,S)-enantiomers, while RET differs by 1.6 times. For (S,S)-, the CIDNP coefficient is almost an order of magnitude greater than for (R,S)- and (S,R)-. To understand the source of this difference, hyperpolarization of (S,S)-and (R,S)- has been calculated using theory involving the electron dipole–dipole interaction in the secular equation.     

基于包络线聚类的多模融合超短期光伏功率预测算法

针对传统功率预测方法以气象因素进行聚类划分时各气象因素权重难以分配以及单模型预测精度较差的问题,提出一种基于光伏功率包络线聚类的多模融合超短期光伏功率预测算法.对异常特征数据进行预处理,采用Pearson相关系数与XGB Feature Importance模块分析光伏功率和各特征之间的相关关系,并构建新特征;介绍包络线理论,并根据光伏功率包络线参数进行聚类划分,将聚类后的数据作为输入,借鉴Stacking集成学习框架构造XGBoost+LightGBM+LSTM融合模型对光伏功率进行预测;将所提算法与气象因素聚类和功率区间聚类下的各预测算法进行实验对比;为了避免训练过程中模型超参数的影响,采用K折交叉验证对数据的训练集、验证集和测试集进行划分.仿真结果表明,所提算法较气象因素和功率区间聚类法能有效提高复杂天气情况下光伏功率预测精度,且多模融合效果总体优于单独算法模型.     

MCCR产线低碳钢高拉速技术研究与应用

通过统计和分析现场数据,得出限制MCCR薄板坯连铸连轧低碳钢拉速提高的主要因素为结晶器热像图中的冷齿和结晶器液面波动,对冷齿和液面波动的成因进行研究,并提出有效控制措施。研究结果表明,结晶器热像图中的冷齿与结晶器弯月面凝固收缩特性相关,受冷却铜板厚度、碳当量、拉速及保护渣影响,反映到铸坯实物上为凹陷或者裂纹缺陷,需合理匹配形成最优参数组合,以降低因冷齿造成的漏钢风险。当结晶器铜板厚度减薄量在6.7%以内时,一冷水维持原设计流量;当结晶器铜板厚度减薄量在6.8%~11.1%时,拉速4.0 m/min以上时需降低10%的一冷水流量;当结晶器铜板厚度减薄量在11.2%~15.6%时,所有拉速下需降低18%的一冷水流量,同时使用高碱度B型保护渣。针对高拉速下结晶器液面波动问题,通过数值模拟研究浸入式水口插入深度、拉速、结晶器断面宽度及电磁制动等参数对结晶器内流场和温度场的影响规律,得到不同拉速和不同断面条件下电磁制动电流的合理配置,使得拉速达到5.5 m/min时钢液面最大流速仍小于0.3 m/s。上述研究结果应用后,结晶器冷齿问题得到有效缓解,110 mm厚的薄板坯最高拉速达到5.8 m/min,结晶器液面波动控制在±1 mm以内,保护渣液渣层厚度保持在8~10 mm,结晶器热流稳定,实现了高拉速的顺稳生产。     

非晶玻璃包裹细丝的制备与铁磁共振性能的应用

非晶玻璃包裹细丝不仅具有电、磁、强度和塑性等方面的显著特点,而且具有突出的自然铁磁共振性能,作为吸波材料具有潜在的应用价值.阐述了玻璃包裹熔融纺丝法制备非晶玻璃包裹细丝的基本原理和实验参数,介绍了细丝产生铁磁共振性能的基本原理,及在吸波材料方面的应用.     

PZT／Terfenol-D／PVDF层状复合磁电材料谐振特性

采用热压法制备出二层锆钛酸铅(PZT)／聚偏氟乙烯(PVDF)与1层Tb0.28Dy0.72Fe2(Terfenol-D)／PVDF叠层结构的三元复合磁电材料，研究了不同PVDF含量，以及不同层厚比tp／L对样品的介电常数、介电损耗、电阻率、谐振频率以及磁电转换系数αE33，αE31的影响规律，结果显示PVDF的含量和层厚比都存在最佳值(最佳值出现在产0．5，tp／L=2／7)，并且在这个最佳值处，磁电转换系数达到最大。     

Detection of water ingress in composite sandwich structures: a magnetic resonance approach

Water ingress inside honeycomb sandwich panels during service has been linked to in-flight failure in some aircraft. There is an ongoing effort to develop nondestructive testing methods to detect the presence of water within the panels. Magnetic resonance (MR) represents an attractive approach in that it is sensitive to moisture. Using a unilateral MR sensor, testing can be applied directly to the surface of the panel. The viability of MR is demonstrated through laboratory imaging of both water within sandwich panels, as well as the adhesive itself. The detection of water using a one-sided handheld MR sensor is presented. It is shown that simple detection, as well as spatial localization of water within sandwich panels is possible.     

丁基罗丹明B-AuI4-缔合纳米粒子体系的极谱猝灭效应研究

在0．1mol／LH2SO4介质中，丁基罗丹明B(RBB)在-0．59伏产生1个单扫描极谱峰。当有AuI^-4存在时，AuI^-4与RBB^ 主要通过静电引力形成疏水性的AuI4—2RBB缔合物分子。AuI4—2RBB存在较强的分子间作用力和疏水作用力而生成紫红色的(AuI4—RBB)n纳米微粒，在470nm处产生1个瑞利散射峰，在610nm处产生1个共振瑞利散射峰；而在-0．59伏处的极谱峰降低。这是由于该紫红色复合纳米微粒形成所致。当纳米微粒体系加入乙醇后，体系的瑞利散射峰和共振瑞利散射峰消失，极谱峰、同步荧光峰和颜色恢复，由于乙醇而致使紫红色的(AuI4-RBB)n纳米微粒分解为红色AuI4—RBB分子。研究结果表明，紫红色(AuI4-RBB)。纳米粒子的形成是其极谱猝灭和共振瑞利散射效应的根本原因。     

用弯曲模型推导二组元复合材料杨氏模量新公式

用弯曲模型推导了适用于悬丝耦合弯曲共振法检测金属第二组元的杨氏模量的理论公式。通过实验数据计算。结果表明在两组元h2/h1≈1时与文献[2]的计算结果几乎接近，为用悬丝耦合弯曲共振法检测各种减振材料，陶瓷材料杨氏模量提供了理论公式；同时用改进后的弯曲模型推导出了适用于纤维复合材料横向杨氏模量的预测公式。     

几种血卟啉3,8—烷氧（硫）基衍生物产生单重态氧能力的研究

在“血卟啉衍生物”(HPD)肿瘤光动力疗法取得令人瞩目成就的同时,化学家们证明了临床使用的HPD制剂并非原意义上的血卟啉衍生物,而是一种复杂的卟啉混合物,其中血卟啉(HP),羟乙基-乙烯基-次卟啉(HVD),原卟啉(PP)约占总量的80％,Dougherty等分离得到其余20％未知结构羧基卟啉,认为此为HPD的有效成分,经进一步分析测定其主要成分是二血卟啉醚(DHE)。但最近的工作又表明,全合成得到的DHE与HPD有效成分的化学组成及生物活性亦不相同。显然这类药物组分复杂、结构的不确定限制了肿瘤光动力疗法的进一步发展。近年来,人们正试图从已知结构的化合物中探索新的光敏药物,作者在合成出一系列结构确定的单一组分血卟啉