分子标记技术及其在植物育种中的应用

概述各种常用分子标记技术RFLP,RAPD,AFLP,SSR,ISSR,SCAR,STS,CAPs的原理,并从种质资源的鉴别、基因定位、基因累加、异源目的基因的筛选、遗传图谱的构建等方面综述了分子标记技术在植物育种中的应用。     

Role of Tea1p, Tea3p and Pom1p in the determination of cell ends in Schizosaccharomyces pombe

Schizosaccharomyces pombe cells are rod-shaped and grow along a single axis from their two ends. Microtubules extend from the cell centre terminating at the cell ends. The ERM(ezrin/radixin/moesin)-like proteins Tea1p and Tea3p, and the Dyrk-like kinase Pom1p are cell end markers involved in the regulation of growth and microtubular dynamics at the cell ends. We have analysed the relative contribution of these three proteins to the determination of cell ends as sites both for cell growth and for microtubular termination. Pom1Delta, in combination with Tea1Delta or Tea3Delta, has the greatest difficulty in relocalizing actin to the cell ends following actin depolymerization and generates the most defective growth pattern. Tea1Delta, in combination with Pom1Delta or Tea3Delta, displays the highest number of microtubules bending round the cell ends. Tea1DeltaPom1Delta, which has the most defective growth pattern and microtubules, also displays the highest number of branched cells. We show that Tea1p, Tea3p and Pom1p all contribute, to different extents, to the determination of cell ends, as sites for both cell growth and microtubular termination. We also show that the fission yeast cell relies on both the positioning of landmarks and a properly organized microtubule cytoskeleton to direct cell growth.     

谈应用SSR分子标记技术和QTL Mapper 1.6 进行作物数量性状基因定位

介绍了SSR分子标记技术和数量基因座位定位软件QTL Mapper1．6，扼要说明如何应用它们进行作物的数量性状基因定位方法。     

基于HPLC与主成分分析法区别西南地区不同产地花椒的地域差异:标记化合物的测定和鉴定

为研究西南地区不同产地花椒的地域差别,本文选取了5个产地花椒的15个样品作为实验材料,采用有机溶剂提取并用柱层析方法分离得到不同的组分。通过高效液相色谱法(HPLC)对分离后的组分进行分析。利用主成分分析(PCA)和偏最小二乘判别分析(PLS-DA),找出汉源花椒与其他产地花椒的主要差异物。结果表明,汉源花椒能够与其他产地花椒明显区分,且PLS-DA的分离效果优于PCA。以VIP（变量重要性）>1及p<0.05为指标筛选出汉源花椒和其他产地花椒的主要差异物为新绿原酸、绿原酸、槲皮苷、羟基-β-山椒素和羟基-γ-山椒素,其中汉源花椒的羟基-γ-山椒素含量显著高于其他产地花椒,其他差异物含量均低于其他产地花椒,差异有显著性（p<0.05）。本研究为不同产地花椒的筛选及品质评价提供了一定的理论基础和实验数据。     

风电-串补输电系统次同步谐振特性与风电机组数量及线路参数关系研究

针对双馈风电-串补系统次同步谐振特性,针对风电-串补系统次同步谐振问题,在RT-LAB中建立风电-串补送出系统等值模型,利用阻抗分析方法,对SSR风险与风电机组数量以及线路参数关系进行研究。得到主要结论:1)在不同风速下,均存在使系统阻尼特性最差的并网风电机组数量;2)线路串补度减小,系统的次同步谐振风险逐渐降低;3)线路长度越大,系统次同步谐振风险随风电机组数量的增加而增加。     

Comparison of radiation‐induced hydrocarbons for the identification of irradiated perilla and sesame seeds of different origins

BACKGROUND: Perilla and sesame seeds, a rich source of energy, are commonly utilized in different forms in many countries. During the post‐harvest period, they are contaminated with insects as well as microbes that may have importance for keeping quality and quarantine, and thus they can be treated with ionizing radiation for insect disinfestation and microbial decontamination. Reliable and routine methods to identify whether or not a food has been irradiated are needed to help consumers' understanding of irradiated food and promote international trade. In the present study, fat‐derived hydrocarbons from irradiated perilla seeds and sesame seeds of Korean and Chinese origin were analyzed in order to identify irradiation treatment by comparing their properties during the post‐irradiation period. RESULTS: Gas chromatographic–mass spectrometric analysis showed that several saturated hydrocarbons, such as tetradecane, pentadecane, hexadecane and heptadecane, were found in the non‐irradiated control samples, while four radiation‐induced unsaturated hydrocarbons (R² = 0.647–0.997), such as 1,7,10‐hexadecatriene (C_16:3), 1,7‐hexadecadiene (C_16:2), 6,9‐heptadecadiene (C_17:2) and 8‐heptadecene (C_17:1), were detected in all irradiated samples at 0.5 kGy or higher, with variations according to sample and origin. Concentrations of all hydrocarbons were reduced during storage and could not be detected in 0.5 kGy irradiated Chinese sample of either seed after 8 months. CONCLUSION: Radiation‐induced hydrocarbons (C_{16:3, 16:2, 17:2, 17:1}) could be used as markers to identify irradiated perilla and sesame seeds of both Korean and Chinese origin at 1 kGy or higher for 8 months' storage at room temperature. Copyright © 2009 Society of Chemical Industry     

Role of Melatonin in Schizophrenia

Schizophrenia is a chronic mental disease that disturbs several cognitive functions, such as memory, thought, perception and volition. Schizophrenia’s biological etiology is multifactorial and is still under investigation. Melatonin has been involved in schizophrenia since the first decades of the twentieth century. Research into melatonin regarding schizophrenia has followed two different approaches. The first approach is related to the use of melatonin as a biological marker. The second approach deals with the clinical applications of melatonin as a drug treatment. In this paper, both aspects of melatonin application are reviewed. Its clinical use in schizophrenia is emphasized.     

Radioimmunoassay of pepsinogens I and II in human serum

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COMPARISON OF VARIOUS CORRELATION ON IRMA FOR CA19-9 AND CA50 IN THE DIGESTIVE SYSTEM TUMOUR

This paper compared various correlation of IMRA for CA19-9 and CA50 in the digestive system tumour antigen, such as linearity, sensitivity, analytical range, precision as well as storage and stability and so on. And it also determined serum level of CA19- 9 and CA50 of patients with different tumours. The results showed it was well correlated to CA19- 9 and CA50 as a marker of digestive system tumour. Particularly high levels of both markers were found in patients with pancreastic colonic cancer. Therefore it is possible to obtain a higher sensitivity and acceptable specificity by combination with CA19-9 and CA50.     

Identification of Apolipoprotein C-I as a Potential Wilms’ Tumor Marker after Excluding Inflammatory Factors

Wilms’ tumor is one of the most common malignant tumors observed in children, and its early diagnosis is important for late-stage treatment and prognosis. We previously screened and identified protein markers for Wilms’ tumor; however, these markers lacked specificity, and some were associated with inflammation. In the current study, serum samples from children with Wilms’ tumors were compared with those of healthy controls and patients with systemic inflammatory response syndrome (SIRS). After exclusion of factors associated with inflammation, specific protein markers for Wilms’ tumors were identified. After comparing the protein peak values obtained from all three groups, a protein with a m/z of 6438 Da was specified. Purification and identification of the target protein using high-pressure liquid chromatography (HPLC) and two-dimensional liquid chromatography-linearion trap mass spectrometry(2D-LC-LTQ-MS) mass spectrometry, respectively, revealed that it was apolipoprotein C-I (APO C-I). Thus, APO C-I is a specific protein marker for Wilms’ tumor.