青霉素发酵过程的智能优化控制

本文在分析青霉素发酵补糖工艺的要求和存在的问题基础上,设计出智能控制算法,采用集散系统实现在线优化控制,经实际应用,效果明显。     

Insertional re-activation of a chloramphenicol acetyltransferase misfolding mutant protein

The deletion of nine residues from the C-terminus of the bacterialchloramphenicol acetyltransferase (CAT) results in depositionof the mutant protein in cytoplasmic inclusion bodies and lossof chloramphenicol resistance in Escherichia coli. This foldingdefect is relieved by C-terminal fusion of the polypeptide withas few as two residues. Based on these observations, efficientpositive selection for the cloning of DNA fragments has beendemonstrated. The cloning vector encodes a C-terminally truncatedCAT protein. Restriction sites in front of the stop codon allowthe insertion of target DNA, resulting in the production ofproperly folded CAT fusion proteins and regained chloramphenicolresistance. The positive selection of recombinants is accomplishedby growth of transformants on chloramphenicol-containing agarplates. The method appears particularly convenient for the cloningof DNA fragments amplified by the PCR because minimal informationto restore CAT folding can be included in the primers. The cloningof random sequences shows that the folding defect can be relievedby fusion to a wide variety of peptides, providing great flexibilityto the positive selection system. This vector may also contributeto the determination of the role of the C-terminus in CAT folding.     

LMG200型可调高滚筒式露天采煤机

介绍了可调高滚筒式露天采煤机的结构特点及在露天煤矿应用所产生的效益和其广 阔的发展前景。     

冷媒充填机的PLC控制

介绍了冷媒充填机控制系统的组成和工作原理，着重叙述了西门子S7－200可编程控制器与触摸屏结合的控制方式，使系统的性能得到较大的提高。     

筒体环缝数控MAG焊接自动机

研究开发的筒体数控MAG自动焊机适应于不同直径，长度和壁厚的筒体的焊接。可预置5种工件的焊接参数且在焊接过程中还可根据情况进行调节。该机适应于多层多道焊，既可以自动排列焊道也可以进行摆动焊接。     

线切割加工轧辊孔型模板提高精度的方法

详细论述了利用线切割机加轧辊孔型模板中易出现的几种影响加工精度的因素及消除方法，以达到提高模板精度。     

数控机床逐点比较法插补直线分析

通过分析四方向逐点比较法的基本原理及基本特点说明其插补精度比较低的原因,提出提高插补精度的方法——八方向逐点比较插补法。     

钻机柴油机的动向和190系列柴油机的发展

本文概括介绍了国内外钻机柴油机发展的水平及动向,比较了国内钻机柴油机与国外钻机柴油机在发展上的差距。重点介绍了作为国产钻机主要动力的190系列柴油机的发展状况及最新技术在该机型上的应用。     

FILIP: A fuzzy intelligent information system with learning capabilities

FILIP (fuzzy intelligent learning information processing) system is designed with the goal to model human information processing. The issues addressed are uncertain knowledge representation and approximate reasoning based on fuzzy set theory, and knowledge acquisition by “being told” or by “learning from examples”. Concepts that can be “learned” by the system can be imprecise (fuzzy), or the knowledge can be incomplete. In the latter case, FILIP uses the concept of similarity to extrapolate the knowledge to cases that were not covered by examples provided by the user. Concepts are stored in the Knowledge Base and employed in intelligent query processing, based on flexible inference that supports approximate matches between the data in the database and the query.

The architecture of FILIP is discussed, the learning algorithm is described, and examples of the system's performance in the knowledge acquisition and querying modes, together with its explanatory capabilities are shown.     

广州抽水蓄能电站厂区消防设计述评

广州抽水蓄能电站是我国第一座高水头，大容量的抽水蓄能电站，该电站按无人值班设计，为此对火灾探测和消防报警以在动灭火系统的自动化程序和可靠性比常规电站有更高的要求，本文重点介绍法国CEGELEG（集团）公司消防设计特点，并对设计中存在的主要问题进行了分析。