Structural Study of the Partially Disordered Full‐Length δ Subunit of RNA Polymerase from Bacillus subtilis

The partially disordered δ subunit of RNA polymerase was studied by various NMR techniques. The structure of the well‐folded N‐terminal domain was determined based on inter‐proton distances in NOESY spectra. The obtained structural model was compared to the previously determined structure of a truncated construct (lacking the C‐terminal domain). Only marginal differences were identified, thus indicating that the first structural model was not significantly compromised by the absence of the C‐terminal domain. Various ¹⁵N relaxation experiments were employed to describe the flexibility of both domains. The relaxation data revealed that the C‐terminal domain is more flexible, but its flexibility is not uniform. By using paramagnetic labels, transient contacts of the C‐terminal tail with the N‐terminal domain and with itself were identified. A propensity of the C‐terminal domain to form β‐type structures was obtained by chemical shift analysis. Comparison with the paramagnetic relaxation enhancement indicated a well‐balanced interplay of repulsive and attractive electrostatic interactions governing the conformational behavior of the C‐terminal domain. The results showed that the δ subunit consists of a well‐ordered N‐terminal domain and a flexible C‐terminal domain that exhibits a complex hierarchy of partial ordering.     

将DNA碱基翻译成蛋白质氨基酸程序设计

基因是生物的遗传物质,蛋白质是生物性状的体现物质,基因通过控制蛋白质的形成来控制生物的性状。根据DNA转录形成RNA,RNA翻译形成蛋白质的规则,利用C语言编写出翻译基因碱基序列成蛋白质氨基酸序列的程序,这无论在遗传学、医学研究,还是C语言程序设计的教学和学习都具有一定的实用价值。     

网络通信中的远程接入技术

介绍了远程接入的定义，应用，描述了远程接入的应用前景，提出了远程接入的解决方案，比较了各解决方案的优缺点，并指出了要解决的问题。     

从海鱼加工下脚料中提取核酸的试验(Ⅱ)——从鱼白中提取核酸及产物纯度分析和组分鉴定

对地产海鱼加工下脚料之一——鱼白中核酸的提取方法、产品纯度测定和组分鉴定方法进行了一些探索。结果显示,DNA粗产物得率为4.62％,DNA质量分数为4.77％。RNA粗产物得率为1.98％,RNA质量分数为4.06％,提取方法基本可行。     

Generalization of a Suffix Tree for RNA Structural Pattern Matching

In molecular biology, it is said that two biological sequences tend to have similar properties if they have similar three-dimensional structures. Hence, it is very important to find not only similar sequences in the string sense, but also structurally similar sequences from databases. In this paper we propose a new data structure that is a generalization of a parameterized suffix tree (p-suffix tree for short) introduced by Baker. We call it the structural suffix tree or s-suffix tree for short. The s-suffix tree can be used for finding structurally related patterns of RNA or single-stranded DNA. Furthermore, we propose an O(n(log|| + log||)) on-line algorithm for constructing it, where n is the sequence length, || is the size of the normal alphabet, and || is that of the alphabet called parameter, which is related to the structure of the sequence. Our algorithm achieves linear time when it is used to analyze RNA and DNA sequences. Furthermore, as an algorithm for constructing the p-suffix tree, it is the first on-line algorithm, though the computing bound of our algorithm is the same as that of Kosarajus best-known algorithm. The results of computational experiments using actual RNA and DNA sequences are also given to demonstrate our algorithms practicality.     

基于免疫粒子群集成的RNA二级结构预测算法

RNA二级结构预测在计算生物学中具有重要意义,针对RNA二级结构预测,提出了一种新的免疫粒子群集成算法,根据个体的浓度和适应值概率,利用免疫机制,在粒子群优化算法中设计了免疫替换算子,有效防止了粒子群优化算法易陷入局部最优的缺陷;通过集成技术,充分发挥各种粒子群优化算法的优点,实现协同演化,提高了算法的全局搜索能力。最后用免疫粒子群集成算法去预测RNA二级结构,实验证明了算法的有效性。     

基于奇异值分解的RNA二级结构相似度计算方法

针对传统的核糖核酸(RNA)分子结构相似度计算方法存在复杂度高、未能充分考虑二级结构全局信息的缺点,基于一种7维序列编码方法，提出了一种利用矩阵奇异值分解提取RNA二级结构数据分布特征的算法，利用提取出的奇异值向量,基于向量距离进行RNA二级结构相似度比对.通过对Yeast tRNA phe、PSTV、poliovirus mutant 3种RNA分子二级结构构象的实验分析，表明了该方法的可行性和高效性.并将这种方法推广至任意两个RNA分子之间二级结构的相似度衡量，同样取得了不错的效果.