Masking the Peroxidase-Like Activity of the Molybdenum Disulfide Nanozyme Enables Label-Free Lipase Detection

Two-dimensional MoS₂ nanoparticles (2D-nps) exhibit artificial enzyme properties that can be regulated at bio-nanointerfaces. We discovered that protein lipase is able to tune the peroxidase-like activity of MoS₂ 2D-nps, offering low-nanomolar, label-free detection and identification in samples with unknown identity. The inhibition of the peroxidase-like activity of the MoS₂ 2D-nps was demonstrated to be concentration dependent, and as low as 5 nm lipase was detected with this approach. The results were compared with those obtained with several other proteins that did not display any significant interference with the nanozyme behavior of the MoS₂ 2D-nps. This unique response of lipase was characterized and exploited for the successful identification of lipase in six unknown samples by using qualitative visual inspection and a quantitative statistical analysis method. The developed methodology in this approach is noteworthy for many aspects; MoS₂ 2D-nps are neither labeled with a signaling moiety nor modified with any ligands for signal readout. Only the intrinsic nanozyme activity of the MoS₂ 2D-nps is exploited for this detection approach. No analytical equipment is necessary for the visual detection of lipase. The synthesis of the water-soluble MoS₂ 2D-nps is low costing and can be performed in bulk scale. Exploring the properties of 2D-nps and their interactions with biological materials reveals highly interesting yet instrumental features that offer the development of novel bioanalytical approaches.     

智能化救援监控系统在煤矿安全生产中的应用

针对煤矿发生事故后传统救援监控系统无法实时对井下人员进行动态定位，导致矿井救援盲目性大、救援效率差、救援难度大等技术难题，为了进一步提高煤矿救援效率，通过技术研究，设计了一套以通信基站为核心的智能化救援监控系统，分析了该系统结构组成、工作原理，通过在担水沟煤矿井下实际应用效果来看，智能化救援监控系统对人员定位精准度达95%，实现人员动态位置三维成像，救援效率提高至80%以上，有效缩短了煤矿事故救援时间，取得了显著应用成效。     

ECG heart beat classification method based on modified ABC algorithm

Electrocardiogram is the most commonly used tool for the diagnosis of cardiologic diseases. In order to help cardiologists to diagnose the arrhythmias automatically, new methods for automated, computer aided ECG analysis are being developed. In this paper, a Modified Artificial Bee Colony (MABC) algorithm for ECG heart beat classification is introduced. It is applied to ECG data set which is obtained from MITBIH database and the result of MABC is compared with seventeen other classifier's accuracy.In classification problem, some features have higher distinctiveness than others. In this study, in order to find higher distinctive features, a detailed analysis has been done on time domain features. By using the right features in MABC algorithm, high classification success rate (99.30%) is obtained. Other methods generally have high classification accuracy on examined data set, but they have relatively low or even poor sensitivities for some beat types. Different data sets, unbalanced sample numbers in different classes have effect on classification result. When a balanced data set is used, MABC provided the best result as 97.96% among all classifiers.Not only part of the records from examined MITBIH database, but also all data from selected records are used to be able to use developed algorithm on a real time system in the future by using additional software modules and making adaptation on a specific hardware.     

“人工智能+”背景下的数据安全与隐私保护探析

随着大数据和云计算的技术的深入应用，人工智能时代的机器学习和深度学习更需要日益增长的数据，因此数据安全与隐私保护变得更加迫切。本文介绍人工智能的定义以及特征，探究数据安全和隐私保护现状，分析数据安全和隐私保护面临的诸多问题，并提出在人工智能时代对数据安全和隐私保护的措施。     

Identification of digital technologies and digitalisation trends in the mining industry

Digitalisation in mining refers to the use of computerised or digital devices or systems and digitised data that are to reduce costs, improve business productivity, and transform mining practices. However, it remains increasingly difficult for mining companies to decide which digital technologies are most relevant to their needs and individual mines. This paper provides an overview of digital technologies currently relevant to mining companies as presented and discussed by mining journals, the media and insight reports of leading consultancy agencies. Relevant technologies were systematically identified using text-mining techniques, and network analyses established the relations between significant technologies. Results demonstrated that currently 107 different digital technologies are pursued in the mining sector. Also, an analysis of the actual implementation of digital technologies in 158 active surface and underground mines reveals a limited uptake of digital technologies in general and that the uptake increases with the run-of-mine production. Large-scale mining operations appear to select and apply digital technologies suitable to their needs, whereas operations with lower production rates do not implement the currently available digital technologies to the same extent. These minor producers may require other digital transformation solutions tailored to their capabilities and needs and applicable to their scale of operations.     

Behaviour of Surface Defects in Wire Rod Rolling

Defects are often present in rolled products, such as wire rod. The market demand for wire rod without any defects has increased. In the final wire rod products, defects originating from steel making, casting, pre‐rolling of billets and during wire rod rolling can appear. In this work, artificial V‐shaped longitudinal surface cracks have been analysed experimentally and by means of FEM. The results indicate that the experiments and FEM calculations show the same tendency except in two cases, where instability due to fairly “round” false round bars disturbed the experiment. FE studies in combination with practical experiments are necessary in order to understand the behaviour of the material flows in the groove and to explain whether the crack will open up as a V‐shape or if it will be closed as an I‐shape.     

一种可替代漂珠的低密度材料

漂珠低密度水泥浆由于密度低、强度高、体系稳定而在油田低压易漏层及低压油气层长封固段固井作业中获得了广泛的应用。但近年来,由于漂珠的产量越来越少,油田低成本要求急需开发代替漂珠的新型减轻材料,以满足配制低密度水泥浆的需求。开发了BCL-10S低密度混合减轻材料,其物化性能与漂珠相当,用它作减轻剂配制的低密度水泥浆性能与用漂珠作减轻剂配制的低密度水泥浆性能相媲美,并已经在长庆油田获得了应用。     

人工颅骨用复合材料中微量甲基丙烯酸甲酯的气相色谱法测定

采用氢气焰离子化检测器(FID),以正丁醇为外标物,PEG-20M为固定相,甲醇作为溶剂,气相色谱法测定复合材料人工颅骨中微量MMA的含量。其相对标准偏差<0.022,平均回收率(n=4)为98.0％～99.1％。此方法操作简便,用于实际样品分析可得到满意的结果。     

人工神经网络预测煤的发热量

应用人工神经网络处理部分广东地区煤质分析数据,建立网络模型,预测出煤的发热量,经实测值与预测值比较,在一定的煤种范围内预测值与实测值比较接近。     

异构数据集成方法研究与实现

为解决异构数据集成的复杂性问题，文章提出了一种异构数据同步的新方法——半集成式中间件法。该方法成功地应用在重庆市社会保障综合信息系统中，具有易于实现、可扩展性、跨平台性和效率高等特点。