对象代理数据库的虚属性查询优化方法磁

为对象代理数据库的虚属性查询提出两种优化方法---表达式提升和虚属性合并。表达式提升将虚属性查询转换为对源类中实属性的查询;虚属性合并是对查询涉及的属于相同源对象的多个虚属性,一次性获取相应源对象的属性值用于虚属性值的计算,避免多次访问同一源对象。通过实验分析了这两种方案对虚属性查询的性能影响,实验结果表明,对于虚属性和代理层数较多的查询,表达式提升和虚属性合并的方法极大提升了查询效率。     

基于.Net架构的科研管理系统

该文对高校科研管理工作进行了详细分析,结合高职院科研工作的特点,设计并开发了基于C/S和B/S混合模式的科研管理信息系统,该系统从某高职院科研管理工作的业务流程和教职工的实际需要出发,设计了相应的功能模块和操作流程,应用了正则表达式技术直接从“知网”提取教职工论文发表信息,节省了数据录入时间. 该系统投入运行近两年,使用结果表明,该科研管理系统使科研管理工作更加规范,工作效率大大提高.     

人sΔBAFF的高效原核表达及纯化

目的克隆TNF家族B细胞激活因子(BcellactivatingfactortotheTNFfamily,BAFF)胞外区134~285(sBAFF134-285)氨基酸残基段缺失突变体(sΔBAFF)的cDNA,并进行表达及纯化。方法以构建的重组质粒pUC19/sBAFF为模板,采用一步反向PCR法,扩增缺失编码sBAFF的142~160位氨基酸的核苷酸序列。经测序证实后,克隆入原核表达载体pQE-80L。经IPTG诱导表达,SDS-PAGE和Westernblot检测表达产物,Ni2+-NTA柱层析纯化目的蛋白。结果经一步反向PCR扩增后得到401bp的DNA片段,该片段序列与GenBank报道的编码人ΔBAFF的胞外区(sΔBAFF)cDNA序列一致。含sΔBAFF的表达载体在大肠杆菌中可表达出相对分子质量为18000的蛋白质并以包涵体的形式存在,经Ni2+-NTA柱层析纯化后得到高纯度的目的蛋白。结论已成功地制备出人sΔBAFF蛋白,为其功能的研究创造了条件。     

基于优化支持向量机的人脸表情分类

分析了支持向量机（support vector machine, SVM）目前主要存在的问题和参数选择对分类性能的影响后, 提出了以改进粒子群算法优化SVM关键参数的优化SVM算法。将加入拥挤度因子的微粒群算法引入到SVM中, 在不牺牲泛化性能的前提下, 对其参数进行优化, 增加了SVM初始化参数的多样性, 减慢了局部搜索, 促进其在全局范围内的寻优搜索, 以有效克服SVM算法过分依赖初始值和容易陷入局部极小值的缺点, 并利用由粗到精的策略构造多层SVM人脸表情分类器, 在提高准确率的基础上加快分类的速度。实验证明, 新算法具有速度快、准确率高的优点。     

一种新颖的汉字字形相似度计算方法

为了利用字形描述技术识别相似汉字, 提出了三元组递归表示的汉字字形相似度计算方法。该算法把汉字表示为汉字结构、字首部件和字尾部件三元组, 以部件为运算对象, 字型结构为运算符, 将汉字描述为前缀表达式。通过建立汉字字形相似度计算递归模型, 使计算过程被逐层分解为原子部件间的相似性比较, 从而更好地降低了计算的复杂性。然后将其用于计算汉字的最佳相似字。实验结果表明, 该方法与人的认知结果吻合度很好, 算法是有效可行的。     

A dual-affinity gene fusion system to express small recombinant proteins in a soluble form: expression and characterization of protein A deletion mutants

A novel gene fusion system to express and purify small recombinantproteins in Escherichia coli has been constructed. The conceptallows for affinity purification of soluble gene products bysequential albumin- and Zn²+-affinity chromatography. The dual-affinitysystem is well suited for expression of unstable proteins asonly full-length protein is obtained after purification andproteins gain proteolytic stability in the fusion protein. Herewe show that the dual-affinity approach can be used for theexpression of various unstable derivatives of a single IgG-bindingdomain based on staphylococcal protein A. Analysis of the proteolyticstabilities and the IgG-binding properties of the differentmutant proteins suggest that the model for the structure ofan IgG-binding domain must be re-evaluated.     

人热休克蛋白70基因的克隆、表达及鉴定

目的克隆人热休克蛋白70(HSP70)基因,构建其原核高效表达载体。方法将PCR扩增的HSP70基因克隆到原核高效表达载体pET-28b中,转化大肠杆菌JM109。挑选阳性克隆,提取质粒pE28b70F,转化大肠杆菌BL21(DE3),IPTG诱导表达目标蛋白。结果在大肠杆菌中成功表达了HSP70,表达量约占细菌总蛋白的22·3%,其中可溶性目标蛋白占上清总蛋白的12%。Westernblot表明该目标蛋白具有与鼠抗人HSP70单抗特异结合的抗原活性。通过Ni2+-NTA亲和柱,从1L诱导产物中纯化出约1520mg重组蛋白,纯度在80%以上。结论已成功表达HSP70,为进一步研究其生物学功能和作用机制奠定了基础。     

Efficient Gene Transfection into Mammalian Cells Mediated by Cross-linked Polyethylenimine

25 kDa branched polyethylenimine (PEI) has successfully been used for in vitro and in vivo gene delivery approaches, but it is cytotoxic. Smaller PEIs are usually non-cytotoxic but less efficient. In order to enhance the gene delivery efficiency and minimize cytotoxicity of PEI, we explored to synthesize cross-linked PEIs with degradable bonds by reacting amines of small branched 2000 Da PEI with small diacrylate (1,4-butanediol diacrylate or ethyleneglycol dimethacrylate) for 2–6 hours. The efficiency of the cross-linked PEIs during in vitro delivering plasmid containing enhanced green fluorescent protein (EGFP) gene reporter and their cytotoxicity were assessed in melanoma B16F10 cell and other cell lines. In vivo gene delivery efficiency was evaluated by direct injection delivery of the EGFP plasmid/cross-linked PEI complexes into mice and by estimating the EGFP expression in animal muscles. Compared to commercially available 25-kDa branched PEI, the cross-linked PEIs reported here could mediate more efficient expression of reporter gene than the 25-kDa PEI control, 19-fold more efficiently in B16F10 cells, 17-fold in 293T cells, 2.3-fold in 3T3 cells, and they exhibited essentially nontoxic at their optimized condition for gene delivery. Furthermore the transfection activity of polyplexs was preserved in the presence of serum proteins. The muscle transfected with the cross-linked PEI prepared here exhibited normal morphology and excellent gene expression. The cross-linked PEIs reported here were evidently more efficient than the commercial 25-kD PEI control and had less cytotoxicity in gene delivery in vitro and in vivo.     

双聚类的关联规则挖掘方法

为了使所有关联规则算法都可用于双聚类挖掘,将双聚类问题转化为关联规则的频繁集挖掘问题.在为双聚类挖掘提供大量算法的同时,不但能获得双聚类,而且还能得到额外的双聚类关联信息.基因表达数据的实验结果证明了其有效性.     

精神世界的富翁——电影《贫民窟的百万富翁》赏析

电影《贫民窟的百万富翁》在第81届奥斯卡颁奖典礼上横扫8个奖项,其内容丰富,构思巧妙,画面对比鲜明,音效烘托强烈。电影成功展示了主人公杰玛.马里克艰难坎坷的生活和内心的执著追求。杰玛虽然生活在印度贫民窟,但却是精神世界的百万富翁。