CRISPR/Cas9-Mediated Generation of Pathogen-Resistant Tomato against Tomato Yellow Leaf Curl Virus and Powdery Mildew

Tomato is one of the major vegetable crops consumed worldwide. Tomato yellow leaf curl virus (TYLCV) and fungal Oidium sp. are devastating pathogens causing yellow leaf curl disease and powdery mildew. Such viral and fungal pathogens reduce tomato crop yields and cause substantial economic losses every year. Several commercial tomato varieties include Ty-5 (SlPelo) and Mildew resistance locus o 1 (SlMlo1) locus that carries the susceptibility (S-gene) factors for TYLCV and powdery mildew, respectively. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) is a valuable genome editing tool to develop disease-resistant crop varieties. In this regard, targeting susceptibility factors encoded by the host plant genome instead of the viral genome is a promising approach to achieve pathogen resistance without the need for stable inheritance of CRISPR components. In this study, the CRISPR/Cas9 system was employed to target the SlPelo and SlMlo1 for trait introgression in elite tomato cultivar BN-86 to confer host-mediated immunity against pathogens. SlPelo-knockout lines were successfully generated, carrying the biallelic indel mutations. The pathogen resistance assays in SlPelo mutant lines confirmed the suppressed accumulation of TYLCV and restricted the spread to non-inoculated plant parts. Generated knockout lines for the SlMlo1 showed complete resistance to powdery mildew fungus. Overall, our results demonstrate the efficiency of the CRISPR/Cas9 system to introduce targeted mutagenesis for the rapid development of pathogen-resistant varieties in tomato.     

Graph-Based Dimensionality Reduction for Hyperspectral Imagery: A Review

Hyperspectral image(HSI) contains a wealth of spectral information, which makes fine classification of ground objects possible. In the meanwhile, overly redundant information in HSI brings many challenges. Specifically, the lack of training samples and the high computational cost are the inevitable obstacles in the design of classifier. In order to solve these problems, dimensionality reduction is usually adopted. Recently, graph-based dimensionality reduction has become a hot topic. In this paper, the graph-based methods for HSI dimensionality reduction are summarized from the following aspects. 1) The traditional graph-based methods employ Euclidean distance to explore the local information of samples in spectral feature space. 2) The dimensionality-reduction methods based on sparse or collaborative representation regard the sparse or collaborative coefficients as graph weights to effectively reduce reconstruction errors and represent most important information of HSI in the dictionary. 3) Improved methods based on sparse or collaborative graph have made great progress by considering global low-rank information, local intra-class information and spatial information. In order to compare typical techniques, three real HSI datasets were used to carry out relevant experiments, and then the experimental results were analysed and discussed.Finally, the future development of this research field is prospected.     

基于BC聚类的差分隐私保护推荐算法

为提高差分隐私保护下推荐算法的准确性,提出了一种考虑差分隐私保护的基于Bhattacharyya系数（BC）的聚类推荐算法.以BC作为项目相似性度量的标准,根据BC相似性对项目进行K-medoids聚类,并在聚类簇中进行私有项目邻居选择.最后,根据最近邻居集信息,对用户的评分进行预测和Top-n推荐.提出的方案有效地克服了已有方法中存在的相似性度量依赖于共同评分的问题,提高了相似性度量的准确性,有效避免了因隐私保护而造成的最近邻居集质量下降的问题.理论分析和实验测试的结果表明,该方法在实现隐私保护的同时还能有效保证推荐的高质量,较好地实现了隐私保护和数据效用之间的平衡,具有良好的应用潜力.     

基于Windows的液压实时测试浅析

本文通过分析WINDOWS实时处理的局限性，提出了解决问题的几种机制；同时以液压元件的实时测试中断程序的实现为例说明了机制的应用，研究表明，这种机制较传统方法实用、有效。     

基于嵌入式Linux的实时优化方案

在分析了国外两种主流嵌入式Linux实时化的实现方法的基础上,根据实时操作系统和Linux的特点,就实时调度策略、细粒度定时器等几个方面提出了改善系统实时性的一种优化方案。     

大型非周期相控阵天线协同设计

针对大型非周期相控阵天线的特点,提出一种多学科、多领域协同设计的方法。然后运用该方法指导两个产品天线设计,围绕协同设计的典型流程和几个关键控制点展开论述。在测试阶段实测天线的主要指标,天线1辐射端面平面度≤1.4 mm,栅瓣电平≤-20 dB,天线2辐射端面平面度≤1.1 mm,栅瓣电平≤-18 dB,实测数据与仿真结果吻合,各项指标优于设计输入要求,使用性能良好,验证协同设计方法的有效性。最后指明大型非周期相控阵天线协同设计的进一步研究方向。     

Next Generation Cereal Crop Yield Enhancement: From Knowledge of Inflorescence Development to Practical Engineering by Genome Editing

Artificial domestication and improvement of the majority of crops began approximately 10,000 years ago, in different parts of the world, to achieve high productivity, good quality, and widespread adaptability. It was initiated from a phenotype-based selection by local farmers and developed to current biotechnology-based breeding to feed over 7 billion people. For most cereal crops, yield relates to grain production, which could be enhanced by increasing grain number and weight. Grain number is typically determined during inflorescence development. Many mutants and genes for inflorescence development have already been characterized in cereal crops. Therefore, optimization of such genes could fine-tune yield-related traits, such as grain number. With the rapidly advancing genome-editing technologies and understanding of yield-related traits, knowledge-driven breeding by design is becoming a reality. This review introduces knowledge about inflorescence yield-related traits in cereal crops, focusing on rice, maize, and wheat. Next, emerging genome-editing technologies and recent studies that apply this technology to engineer crop yield improvement by targeting inflorescence development are reviewed. These approaches promise to usher in a new era of breeding practice.     

产品形态设计中的协同工作研究

计算机支持的协同工作CSCW(Computer Supported Cooperrative Work)随着计算机和通信突飞猛进的发展已经成为近些年来的热点课题,在产品形态设计中开展研究协同工作技术就具有非常重要的意义.文章针对产品设计协同工作的需求,结合产品设计的特点,研究建立了产品形态设计协作模型,论述了基于Web的开发应用技术和开发特征.为解产品形态设计的协同问题,提供了可行的新模式,给出了利用Web现有资源以及低成本技术构建产品开发项目协同设计环境的一种新的开发研究方向.     

Determination of tetrachloroethylene—Results of a collaborative study and the standardized method

The development, by collaborative study, of a standardized method for the determination of tetrachloroethylene in olive oils is described. The results of the study, which was carried out in 1989 under the direction of the International Olive Oil Council (IOOC), are presented and show that tetrachloroethylene can be readily determined, to an acceptable degree of precision, by gas-liquid chromatography with electron capture detection. A limited number of results obtained for the determination of trichloroethylene and chloroform by the same method indicate that a similar precision could be obtained with the method when determining these solvents also.