Feasibility study on the use of polyallyldiglycol-carbonate cell dishes in TUNEL assay for alpha particle radiobiological experiments

In the present work, we have studied the feasibility of a method based on polyallyldiglycol-carbonate (PADC) films to investigate the effects of alpha particles on HeLa cervix cancer cells. Thin PADC films with thickness of about 20 μm were prepared from commercially available CR-39 films by chemical etching to fabricate custom-made petri dishes for cell culture, which could accurately record alpha particle hit positions. A special method involving “base tracks” for aligning the images of cell nuclei and alpha particle hits has been proposed, so that alpha particle transversals of cell nuclei can be visually counted. Radiobiological experiments were carried out to induce DNA damages, with the TdT-mediated dUTP Nick-End Labeling (TUNEL) fluorescence method employed to detect DNA strand breaks. The staining results were investigated by flow cytometer. The preliminary results showed that more strand breaks occurred in cells hit by alpha particles with lower energies. Moreover, large TUNEL positive signals were obtained even with small percentages of cells irradiated and TUNEL signals were also obtained from non-targeted cells. These provided evidence for the bystander effect.     

新安江模型参数优选的改进粒子群算法

借鉴竞争演化和多种群混合的思想,对粒子群算法(PSO)进行改进,建立并行种群混合进化的粒子群算法(PMSE-PSO)和序列主-从种群混合进行的粒子群算法(SMSE-PSO)。数值模拟结果表明,这两种改进的粒子群算法具有较高的计算效率、较强的自适应性和稳定性。将PMSE-PSO和SMSE-PSO应用于新安江模型的参数优选中,通过与PSO和SCE-UA的比较可以看出,PMSE-PSO和SMSE-PSO不仅具有较好的全局优化性能和稳定性,而且在调用目标函数次数相同的情况下精度较高,是一种有效的新安江模型参数优选方法。     

Solid State Concepts to Understand Catalysis Using Nanoscale Metallic Particles

New advances in theoretical background and recent experimental results concerning nanometer scale metallic clusters are presented. We will begin by a presentation of the advantages and the limitations of synchrotron radiation techniques to characterise nanometer scale entities. Then, through a review of recent papers, we try to make a link between metallic surfaces and nanometer scale monometallic clusters in the case of the interaction with a small molecule, namely NO.     

SPECTRAL DENSITY ESTIMATION FROM NONLINEARLY OBSERVED DATA

Abstract. The estimation of the spectral density function of a stationary Gaussian process at the input of an instantaneous nonlinearity is considered when the nonlinearity is known and a finite set of observations of the output process is given. A class of spectral estimates is considered and their quadratic-mean consistency is established; precise asymptotic expressions for their bias and covariance are derived and their asymptotic normality is obtained.     

Approximate channel identification via δ‐signed correlation

A method of approximate channel identification is proposed that is based on a simplification of the correlation estimator. Despite the numerical simplification (no multiplications or additions are required, only comparisons and an accumulator), the performance of the proposed estimator is not significantly worse than that of the standard correlation estimator. A free (user selectable) parameter moves ‘smoothly’ from a situation with small sum‐squared channel estimation error but hard‐to‐identify channel peaks, to one with a larger sum‐squared estimation error but easy‐to‐identify channel peaks. The proposed estimator is shown to be biased and its behaviour is analysed in a number of situations. Applications of the proposed estimator to sparsity detection, symbol timing recovery and to the initialization of blind equalizers are suggested. Copyright © 2002 John Wiley & Sons, Ltd.     

水利工程建设项目的风险分析

水利工程建设中潜藏着众多风险，对风险进行有效分析是工程顺利建设的重要保证。水利工程风险分析是由风险识别、估算、评价、管理、控制决策等组成的科学体系。明确了水利工程中风险的定义，识别了水利工程的风险因素，论述了风险估算和评价的方法，并提出对工程风险进行管理和控制，以保证工程建设顺利进行。     

超微粒子的量子阻尼振动理论

根据SU(1,1)Lie代数,建立超微粒子的量子阻尼振动理论,得到超微粒子量子阻尼振动系统的能级和波函数。     

湖泊藻类叶绿素-α和悬浮物浓度的高光谱定量遥感模型研究

本文利用地物光谱仪对太湖水体进行了光谱测量和同步采样分析，对获得的数据尝试用光谱分离法进行分析，从中分离出蓝藻和悬浮物的特征波峰，建立波峰高度与同步水质采样得到的叶绿素-α浓度和悬浮物浓度的对应关系，得出其遥感定量反演算法，并实际应用在OMIS成像光谱仪图像上．     

用激光粒度分析仪测定二氧化锆粒度

介绍以六偏磷酸钠作为分散剂，采用激光粒度分析仪测量二氧化锆粒度的一种新方法。该方法的测量范围为0．05～300μm。     

Empirical Evaluations of Usage-Based Reading and Fault Content Estimation for Software Inspections

This thesis describes six empirical investigations of two techniques applied to software inspections, namely reading techniques and fault content estimation techniques. The first part of the thesis presents a series of experiments of a novel reading technique called usage-based reading. The second part investigates fault content estimation applied to the software inspection process. The estimation methods used are capture-recapture and curve fitting.