Apicobasal Surfaceome Architecture Encodes for Polarized Epithelial Functionality and Depends on Tumor Suppressor PTEN

The loss of apicobasal polarity during the epithelial-to-mesenchymal transition (EMT) is a hallmark of cancer and metastasis. The key feature of this polarity in epithelial cells is the subdivision of the plasma membrane into apical and basolateral domains, with each orchestrating specific intra- and extracellular functions. Epithelial transport and signaling capacities are thought to be determined largely by the quality, quantity, and nanoscale organization of proteins residing in these membrane domains, the apicobasal surfaceomes. Despite its implications for cancer, drug uptake, and infection, our current knowledge of how the polarized surfaceome is organized and maintained is limited. Here, we used chemoproteomic surfaceome scanning to establish proteotype maps of apicobasal surfaceomes and reveal quantitative distributions of, i.e., surface proteases, phosphatases, and tetraspanins as potential key regulators of polarized cell functionality. We show further that the tumor suppressor PTEN regulates polarized surfaceome architecture and uncover a potential role in collective cell migration. Our differential surfaceome analysis provides a molecular framework to elucidate polarized protein networks regulating epithelial functions and PTEN-associated cancer progression.     

头花蓼多酚不同极性溶剂萃取物的抗氧化活性

目的:比较头花蓼多酚不同极性溶剂萃取物抗氧化活性并考察活性与多酚含量的相关性。方法:将头花蓼提取物过大孔树脂纯化后得到头花蓼精多酚样品,采用不同极性有机溶剂对头花蓼精多酚样品进行液-液萃取,将头花蓼多酚样品分为石油醚相、氯仿相、乙酸乙酯相、正丁醇相、水相不同极性溶剂萃取物。测定不同极性溶剂萃取物的总多酚含量,研究各萃取物清除DPPH自由基、羟自由基及ABTS自由基能力,分析抗氧化活性与多酚含量关系,并采用HPLC对乙酸乙酯萃取物进行初步鉴定。结果:头花蓼不同极性萃取物均有抗氧化活性,乙酸乙酯相抗氧化活性最好,清除DPPH、ABTS、羟自由基的IC₅₀值与多酚含量呈现相关性(r=-0.988,r=-0.742,r=-0.563),槲皮苷是乙酸乙酯相的主要抗氧化成分之一。结论:乙酸乙酯相提取物可作为分离头花蓼抗氧化活性物质的重点,多酚是抗氧化的主要活性成分。     

蓝宝石衬底上原子级光滑AlN外延层的MOCVD生长

在蓝宝石衬底表面无氮化、低Ⅴ/Ⅲ比的情况下,采用1200℃的衬底温度、5kPa反应室气压,用MOCVD方法在蓝宝石衬底上生长出了表面原子级光滑的AlN外延层.原子力显微镜测试表明其平均粗糙度为0.44nm,X射线衍射(0002)回摆曲线FWHM为166".实验结果和分析表明,极性和气相反应是影响AlN表面形貌的主要原因.以原子级光滑的AlN为模板生长出了高质量的高Al组分的n型AlGaN,证实了AlN模板具有较好的质量.     

变极性等离子弧焊铝合金厚板工艺研究

变极性等离子弧焊是一种新型焊接方法,由于等离子弧对焊接工艺和规范参数的变化比较敏感,获得良好焊缝接头的合理规范参数范围窄、裕度小.本文通过工艺试验,探讨了变极性等离子弧焊16mm铝合金厚板的技术可行性,论述了其相对于传统弧焊工艺的优点.     

综合暂态与工频信息的谐振接地系统小电流接地故障选线

对于谐振接地系统,利用工频电流的小电流接地故障选线方法不再适用,而受现场普遍存在的电压互感器/电流互感器接线极性反接问题影响,利用暂态电流极性比较与暂态无功功率方向的选线方法也易出现误选。文中在分析过补偿方式谐振接地系统中接地故障暂态与工频零序电流/无功功率分布差异的基础上,提出两种综合利用故障暂态与工频分量的全信息量选线方法,即:与任一其他线路暂态零序电流极性关系与工频零序电流极性关系均不一致的线路为故障线路;或暂态无功功率与工频无功功率流向不一致的线路为故障线路。两种方法均可有效避免因电压互感器/电流互感器接线极性反接或不确定造成的误选,扩大了对现场条件的适用性,提高了选线可靠性。仿真与现场数据验证了算法的有效性。     

Formation polarity dependent improved resistive switching memory characteristics using nanoscale (1.3 nm) core-shell IrOx nano-dots

ABSTRACT: Improved resistive switching memory characteristics by controlling the formation polarity in an IrOx/Al2O3/IrOx-ND/Al2O3/WOx/W structure have been investigated. High density of 1 × 1013/cm2 and small size of 1.3 nm in diameter of the IrOx nano-dots (NDs) have been observed by high-resolution transmission electron microscopy. The IrOx-NDs, Al2O3, and WOx layers are confirmed by X-ray photo-electron spectroscopy. Capacitance-voltage hysteresis characteristics show higher charge-trapping density in the IrOx-ND memory as compared to the pure Al2O3 devices. This suggests that the IrOx-ND device has more defect sites than that of the pure Al2O3 devices. Stable resistive switching characteristics under positive formation polarity on the IrOx electrode are observed, and the conducting filament is controlled by oxygen ion migration toward the Al2O3/IrOx top electrode interface. The switching mechanism is explained schematically based on our resistive switching parameters. The resistive switching random access memory (ReRAM) devices under positive formation polarity have an applicable resistance ratio of > 10 after extrapolation of 10 years data retention at 85°C and a long read endurance of 105 cycles. A large memory size of > 60 Tbit/sq in. can be realized in future for ReRAM device application. This study is not only important for improving the resistive switching memory performance but also help design other nanoscale high-density nonvolatile memory in future.     

变极性TIG焊接电源的研制

变极性TIG焊接电源是一种用于铝合金焊接的新型焊接设备，其焊接电流频率、正负半波电流时间和幅值都可以分别独立调节。主电路结构采用2级逆变，前级为全桥逆变式结构，后级为半桥逆变式结构。该电源可实现变极性方波交流、直流、脉冲电流等多处输出。在DCEN向DCEP过渡时采用叠加电流脉冲进行稳弧，小电流时焊接电弧也十分稳定。调节正负半波的幅值、频率及占空比能满足铝及铝合金的焊接工艺要求。     

Novel three‐phase current‐regulated digital PWM and its behavioral analysis

There have been various studies of PWM algorithms for a three‐phase voltage‐source AC/DC power converter since the analog modulation scheme based on a triangular carrier wave was proposed in the 1960s. The PWM algorithm can be considered the heart of electronic power conversion. With progress in digital technology, there is an increasing need for gate signals to be generated directly by digital ICs, such as MPU, DSP, or FPGA/CPLD. This paper analyzes quantitatively the precision of current control of digital PWM taking account of both the sampling period and the delay time (the latter is inevitably accompanied by a digital procedure). The delay time is shown to have a double effect on the current error. In addition, the paper theoretically derives the conditions for digital PWM to meet the PPCR (Pulse Polarity Consistency Rule, that is, the next gate command moves only to the adjacent ones or commands). So far as the authors know, no paper has presented the mathematical requirements for PPCR taking account of the effect of the delay time of digital PWM. The derived theoretical results are summarized as digital PWM design criteria for a three‐phase PWM converter in order to facilitate practical implementation of the theory, guaranteeing PPCR behavior as well as quantitative accuracy of current regulation. © 2004 Wiley Periodicals, Inc. Electr Eng Jpn, 150(2): 62–77, 2005; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.10330     

WMZ-41A型微机母线保护装置的探索与应用

根据WMZ-41A型微机母线保护装置的工作原理,结合胜利油田电力管理总公司九分场变电站220 kV微机母线保护改造施工中遇到的问题,介绍了WMZ-41A型微机母线保护装置的功能及特点,具体分析了单元极性和失灵判据存在的缺陷和原因,提出了改进技术方案,不但提高了施工调试的技术水平,而且在保护的准确性、系统的稳定性和供电的可靠性上都有了质的飞跃。