Evaluation and Characterization of Post-Stroke Lung Damage in a Murine Model of Cerebral Ischemia

After stroke and other brain injuries, there is a high incidence of respiratory complications such as pneumonia or acute lung injury. The molecular mechanisms that drive the brain-lung interaction post-stroke have not yet been elucidated. We performed transient middle cerebral artery occlusion (MCAO) and sham surgery on C57BL/6J mice and collected bronchoalveolar lavage fluid (BALF), serum, brain, and lung homogenate samples 24 h after surgery. A 92 proteins-panel developed by Olink Proteomics^® was used to analyze the content in BALF and lung homogenates. MCAO animals had higher protein concentration levels in BALF than sham-controls, but these levels did not correlate with the infarct volume. No alteration in alveolar-capillary barrier permeability was observed. A total of 12 and 14 proteins were differentially expressed between the groups (FDR < 0.1) in BALF and lung tissue homogenates, respectively. Of those, HGF, TGF-α, and CCL2 were identified as the most relevant to this study. Their protein expression patterns were verified by ELISA. This study confirmed that post-stroke lung damage was not associated with increased lung permeability or cerebral ischemia severity. Furthermore, the dysregulation of HGF, TGF-α, and CCL2 in BALF and lung tissue after ischemia could play an important role in the molecular mechanisms underlying stroke-induced lung damage.     

有杆抽油系统无级调冲次工艺研究

利用变频调速无级调冲次技术对有杆抽油系统采油工艺参数进行了综合研究及评价,结合最新研制生产的抽油机变频调速智能控制装置,分别进行了厂内台架试验及油田现场试验,从不同角度分析研究了抽油机无级调冲次工艺对抽油机平衡度、输入功率、电动机功率因数、油井日产液量、吨油耗电量以及抽油机泵效、系统效率等参数的影响。研究结果表明,在有杆抽油系统的抽油机、抽油杆、油管及抽油泵等配置保持不变的前提下,采用有杆抽油系统无级调冲次工艺可以优选抽油机最佳抽汲参数,并以此提高抽油泵泵效和系统效率,降低吨油耗电量,使有杆抽油系统的泵送能力不断与变化的井底流入条件相匹配,从而在一定程度上提高原油采收率。     

雷电斜向放电通道电磁场建模及计算

基于传输线模型,通过将柱坐标系进行旋转变换,建立了斜向放电通道电磁场模型;采用脉冲函数加双指数函数作为通道底部电流,从垂直放电通道电磁场的表达式中推导出斜向放电通道电磁场的计算公式;基于斜向放电通道电磁场模型,研究了不同角度对不同场区地表回击电磁场的影响,认为雷电放电通道越倾斜,雷电流对地面电磁场的影响就越明显。研究了不同回击速度对不同场区地表回击电磁场的影响,结果表明回击速度对电磁场的影响较为明显。     

破片动态飞散速度的计算

导弹攻击TBM等目标时,在中低空遭遇、大脱靶量、高相对速度情况下,战斗部爆炸后破片动态飞散弹道出现弯曲甚至存在多值问题,以往不考虑这一情况的有关破片动态飞散速度的计算模型已不适用.为此,建立了考虑破片弹道弯曲及多值问题,且适用于任意遭遇情况的导弹战斗部破片动态飞散速度计算模型.模型采用了计算机数值搜索求解方法,解决了复杂弹目交会情况下无法获得飞散速度的解析表达式的问题.利用两个算例,对建立的模型进行了验证,仿真计算结果与实际一致,表明模型是合理、正确的.     

炮弹引信炮口保险性能指标分析

炮弹引信炮口保险性能是一项重要的安全性能 ,因而其设计指标也一直是广泛关注的焦点。本文根据《引信安全性设计准则》关于延期解除保险的要求 ,运用弹丸弹道学、爆炸力学和威力设计理论 ,分别从爆炸冲击波和破片杀伤两个方面分析了弹丸安全距离的大小 ,并依此讨论了炮弹引信的炮口保险性能指标。炮弹引信炮口保险距离设计指标不但与弹丸威力有关 ,还涉及弹丸初速。而影响炮弹引信炮口保险性能指标的弹丸特性主要是破片杀伤作用。对于中大口径火炮榴弹和小口径舰炮、高炮榴弹 ,引信炮口保险指标一般可按弹径的4 0 0倍确定。对于初速较低且炸药装填量又较大的某些航炮榴弹 ,引信炮口保险指标从战术需求角度来看则应远一些 ,应达 1 5m甚至 50 m。弹头引信炮口保险距离指标应比弹底引信长 ,预制破片弹引信炮口保险距离指标应比普通榴弹引信的长     

一种门户环境下的缓存模型和算法

提出的门户环境下的缓存模型和算法的基本思想是把门户页面分解为I序列、布局块和内容块.用户请求页面时,门户系统只生成和传递发生改变的块,再由客户端根据这些块和I序列组装成完整的页面.实验结果表明,该算法可以提高网络带宽的利用率,缩短响应时间.     

Sulfated Hyaluronan Binds to Heparanase and Blocks Its Enzymatic and Cellular Actions in Carcinoma Cells

We examined whether sulfated hyaluronan exerts inhibitory effects on enzymatic and biological actions of heparanase, a sole endo-beta-glucuronidase implicated in cancer malignancy and inflammation. Degradation of heparan sulfate by human and mouse heparanase was inhibited by sulfated hyaluronan. In particular, high-sulfated hyaluronan modified with approximately 2.5 sulfate groups per disaccharide unit effectively inhibited the enzymatic activity at a lower concentration than heparin. Human and mouse heparanase bound to immobilized sulfated hyaluronan. Invasion of heparanase-positive colon-26 cells and 4T1 cells under 3D culture conditions was significantly suppressed in the presence of high-sulfated hyaluronan. Heparanase-induced release of CCL2 from colon-26 cells was suppressed in the presence of sulfated hyaluronan via blocking of cell surface binding and subsequent intracellular NF-κB-dependent signaling. The inhibitory effect of sulfated hyaluronan is likely due to competitive binding to the heparanase molecule, which antagonizes the heparanase-substrate interaction. Fragment molecular orbital calculation revealed a strong binding of sulfated hyaluronan tetrasaccharide to the heparanase molecule based on electrostatic interactions, particularly characterized by interactions of (−1)- and (−2)-positioned sulfated sugar residues with basic amino acid residues composing the heparin-binding domain-1 of heparanase. These results propose a relevance for sulfated hyaluronan in the blocking of heparanase-mediated enzymatic and cellular actions.     

防空反导破片杀伤战斗部现状与发展

通过对防空反导破片杀伤战斗部发展历史的回顾、技术现状的总结分析,基于现代制导和引战水平的发展,防空反导破片杀伤战斗部技术的主要发展趋势为：通过爆轰波形控制提高装药能量利用率、利用新结构提高破片穿孔密度和压缩其分布宽度、采用活性材料破片提高毁伤威力,依此重点发展的破片杀伤战斗部技术为：定向破片战斗部技术、线列式破片战斗部技术和活性破片战斗部技术。     

油气井用隔板起爆器的起爆能力

为了提高油气井用隔板起爆器的起爆能力,在传统隔板起爆器的基础上,改变了其受主装药盲孔的几何结构和受主装药结构,设计出一种油气井用隔板起爆器,并对其进行了起爆能力的实验研究.实验结果显示,油气井用隔板起爆器起爆油气井耐温传爆管的最大空气隙距离为85 mm,而传统隔板起爆器和油气井耐温传爆管分别为20 mm和65 mm,因...     

多种钢制破片侵彻性能的数值模拟研究

为了清楚了解不同形状的预制破片对铝合金靶板的侵彻规律,采用LS-DYNA动力有限元分析软件,对几种典型形状破片侵彻多层间隔靶板进行了数值模拟研究。旨在通过几种不同类型破片的侵彻规律研究,揭示不同破片形状以及入射角所造成的侵彻效果的差别,分析新型钢材料下不同形状预制破片的侵彻效能和多威胁下通用车辆及简易装甲目标的损伤模式。