蜂胶黄酮提取方法研究

设计正交实验，采用水浴和超声分别对蜂胶中黄酮类物质的提取方法进行了研究。结果表明：水浴法以25倍原料重的70%乙醇水溶液在75℃浸提lh为最佳，超声法以20倍原料重的75％乙醇水镕液超声2min浸提为最佳，超声法优于水浴法，黄酮提取率高达45．6％。     

蜂胶对酸奶保鲜效果的研究

在乳制品中,酸奶的微生物污染常常被人们所忽视.作者研究了不同浓度的蜂胶水溶液对酸奶的保鲜效果.实验结果表明,蜂胶水溶液浓度越大,对酵母菌的抑制作用越强,即对酸奶的保鲜效果也越好.添加浓度为0.05%的蜂胶水溶液在延长酸奶保存期的同时,还能保证酸奶自身的品质,因此蜂胶可以作为天然的食品保鲜剂.该研究为蜂胶对各种乳制品的保鲜应用提供了参考.     

蜂胶对牛乳保鲜作用的研究

以蜂胶对牛乳的保鲜作用为基础,探讨了蜂胶的添加方式和浓度对牛乳的酸度和细菌总数的作用效果．将0．2％的蜂胶添加到经巴氏灭菌（65℃,30min）后的牛乳中,牛乳酸度变化最慢,在保质期内可控制到17．5。T,细菌总数可以控制在8．5×10^8个cfu／mL．该研究为蜂胶保鲜剂在牛乳中的应用提供了理论参考．     

天然蜂胶对自由基的清除作用

研究不同溶剂蜂胶提取物对二苯代苦味酰自由基 (DPPH)的清除作用 ,同时用硫代巴比妥酸 (TBA)法测定其对亚油酸的抗氧化性。实验表明 ,体积分数为 95 %乙醇提取物对DPPH自由基的清除作用强于其他提取物。其最佳提取工艺为 ,体积分数 95 %乙醇于 85℃浸提 3h。初步鉴定出蜂胶中含有黄酮、查耳酮、异黄酮、黄酮醇、二氢黄酮等物质。TBA法测定的结果同DPPH法结果一致     

Accumulation of thiobarbituric acid‐reactive substances in cured pork sausages treated with propolis extracts

Propolis collected by bees has many components that possess antifungal, anticancer and antioxidant properties. Cured ground pork meat was treated separately with 0.4% w/w ethanol‐extracted propolis (EEP 0.4%), 0.6% w/w water‐extracted propolis (WEP 0.6%), 0.8% w/w dried residue of ethanol‐extracted propolis (DREEP 0.8%) and 0.1% w/w potassium sorbate (PS 0.1%) and made into sausages. Thiobarbituric acid‐reactive substances (TBARS) were measured in the sausages to assess the effects of storage at 5, 10 and 20 °C after 0, 2 and 4 weeks. After 4 weeks of storage at 5 °C the sausages treated with EEP 0.4%, DREEP 0.8%, WEP 0.6% and PS 0.1% yielded TBARS values which were 50, 59, 35 and 91% respectively of the control value. There was an increase of 0.33 mg MDA kg^?1, the lowest of all treatments, in TBARS for the 0.4% w/w EEP‐treated sausages. The curing process will control microbiological spoilage, as will potassium sorbate. © 2002 Society of Chemical Industry     

研制蜂胶冻干粉的最佳工艺研究

本文探讨了以粗蜂胶为原料,研制蜂胶冻干粉的的工艺。结果表明：蜂胶冻干粉的工艺流程为粗蜂胶—→醇溶法提取—→赋形—→冻干—→冻干粉。最佳工艺条件为：（1）醇溶法提取蜂胶的最佳条件：固液比：1：25,乙醇溶液浓度75％,提取温度80℃,提取时间1．5h。（2）赋形剂为22％甘露醇,赋形的最佳条件为：22％甘露醇与4％的蜂胶乙醇溶液按76：24的比例混合。（3）冻干条件：预冻温度-74℃,预冻时间2小时,抽真空时间24小时,升温干燥20℃,升温干燥30分钟,冷阱温度为-52℃。在此条件下生产的蜂胶冻干粉的溶解度为87％,水分含量为0．93,总黄酮含量为0．49％。     

研制蜂胶冻干粉的最佳工艺研究

本文探讨了以粗蜂胶为原料,研制蜂胶冻干粉的的工艺。结果表明:蜂胶冻干粉的工艺流程为粗蜂胶─→醇溶法提取─→赋形─→冻干─→冻干粉。最佳工艺条件为:(1)醇溶法提取蜂胶的最佳条件:固液比:1:25,乙醇溶液浓度75%,提取温度80℃,提取时间1.5h。(2)赋形剂为22%甘露醇,赋形的最佳条件为:22%甘露醇与4%的蜂胶乙醇溶液按76:24的比例混合。(3)冻干条件:预冻温度-74℃,预冻时间2小时,抽真空时间24小时,升温干燥20℃,升温干燥30分钟,冷阱温度为-52℃。在此条件下生产的蜂胶冻干粉的溶解度为87%,水分含量为0.93,总黄酮含量为0.49%。     

大鼠灌胃中国蜂胶醇提物后血浆中5种酚酸类成分的HPLC测定及其药代动力学

对大鼠灌胃蜂胶乙醇提取物（EEP）后,血浆中5种酚酸类成分的药代动力学特性进行研究.用HPLC-UV法测定血浆中咖啡酸、香豆酸、阿魏酸、异阿魏酸和3,4-二甲氧基肉桂酸的浓度.应用WinNonlin非典型房室模型计算软件计算相应的药代动力学参数.血浆中内源性物质和内标对酚酸类成分测定无干扰,回收率、准确度及日内、日间精密度均符合生物样品测定要求.结果表明,该方法简便、快速、重复性好,适用于咖啡酸等5种蜂胶中酚酸类成分的大鼠血药质量浓度测定及体内药动学研究.     

Precursor system of liquid crystalline phase containing propolis microparticles for the treatment of periodontal disease: development and characterization

Precursor systems of liquid crystalline phase were prepared using the surfactant PPG-5-Ceteth-20, isopropyl myristate, and water; gelatin microparticles containing propolis were then added into these systems. Homogeneity of dispersion, the in-system microparticle morphology, and sedimentation behavior of each formulation were evaluated. The rheological and mechanical properties (hardness, compressibility, and adhesiveness), the work of syringing, and the propolis release profile were also evaluated. All the formulations exhibited pseudoplastic flow and thixotropy, and they displayed storage modulus, loss modulus, dynamic viscosity, and loss tangent that depended on temperature, frequency, and composition. Mechanical properties varied significantly among the formulations being affected by changes in the composition and temperature. Raising the concentration of surfactant and adding propolis microparticles significantly decreased the work of syringing. The drug release was non-Fickian (anomalous) and there was no significant difference between the tested systems in the times required for 10%, 30%, and 50% release of the initial drug loading.     

蜂胶香皂中黄酮成分的测定

采用分光光度比色法测定蜂胶中的总黄酮含量。使用无水乙醇萃取蜂胶香皂中的黄酮成分,用硝酸铝作为显色剂,在415 nm处进行比色,与标准曲线相对比进行定量计算。本方法最低可以测定总黄酮含量在0.06%的蜂胶香皂样品。