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31.
CpG序列及阳离子脂质体对猪带绦虫抗原基因免疫应答的影响 总被引:4,自引:0,他引:4
本实验将猪带绦虫抗原基因VTS76分别与pUC18质粒,重组pUC18-CpG质粒联合及阳离子脂质体包装后免疫小鼠,结果表明:pUC18和pUC18-CpG能显著提高免疫小鼠总IgG和特异性抗体水平,增加小鼠免疫细胞数量,增强淋巴细胞增殖活性及白细胞介素-2的诱生活性,其中,pUC18-CpG的免疫增强效果更显著,阳离子脂质体包裹具有减少质粒用量,提高免疫应答水平的作用。 相似文献
32.
脂质体包裹猪白细胞介素6基因对猪带绦虫抗原基因免疫小鼠的影响 总被引:3,自引:0,他引:3
比较研究了BALb/c小鼠分别肌肉注射猪带绦虫抗原基因VTS76、猪白细胞介素6(VPIL-6)联合VTS76及其阳离子脂质体包裹质粒等5个处理的免疫效应。结果表明,VTS76联合VPIL-6共注射小鼠的IgG含量和抗体滴度,脾细胞增殖反应和IL-2诱生活性以及免疫细胞数量,均比注射VTS76的显著增强。经阳离子脂质体包裹的VTS76+VPIL-6在减少质粒用量80%时,免疫应答水平仍显著高于脂质体包裹VTS76,且明显高于VTS76+VPIL-6,证明VPIL-6能显著增强基因疫苗(VTS76)免疫小鼠的免疫应答。 相似文献
33.
The mechanisms governing the solubilizing interactions between zwitterionic/anionic mixed surfactant systems at different
molar fractions of the zwitterionic surfactant (Xzwitter) and neutral or electrically charged unilamellar liposomes were investigated. The mixed systems were formed by N-dodecyl-N,N-dimethylbetaine
and sodium dodecyl sulfate in the presence of piperazine-1,4-bis-(2-ethanesulfonic acid) buffer at pH 7.20. Unilamellar liposomes formed by egg phosphatidylcholine, in some cases together
with stearylamine or phosphatidic acid, were used. Solubilization was detected as a decrease in static light-scattering of
liposomes. Two parameters were regarded as corresponding to the effective surfactant/lipid molar ratios (Re) at which the
surfactant system (i) saturated the liposomes, Resat, and (ii) led to a total solubilization of liposomes, Resol. From these parameters the bilayer/aqueous medium surfactant partition coefficients for the saturation (Ksat) and complete bilayer solubilization (Ksol) were determined. When Xzwitter was 0.40, The Re and K parameters showed a maximum, whereas the critical micellar concentration (CMC) of these systems exhibited
a minimum, regardless of the electrical charge of bilayers. Given that the ability of the surfactant systems to saturate or
solubilize liposomes is inversely related to the Resat and Resol parameters, these capacities appear to be directly correlated with the CMC of the mixed systems. The similarity of both Ksat and Ksol (particularly for Xzwitter=0.2–0.8) suggests that a similar partition equilibrium governs both the saturation and the complete solubilization of bilayers,
the free surfactant concentration (Sa,Sb), remaining almost constant with similar values to the CMC for each mixed system studied. 相似文献
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37.
LIANG Zhihong ZHAO Yong DENG Xia HE Gang NIE Rongrong CHEN Zhiqing 《武汉理工大学学报(材料科学英文版)》2005,20(B12):60-62
The bio- inspired strategy of triggered release of Ca^2+ , Pi, F from prosomal compartments was used to induce rapid formation of FHA for potential dental use : Calcium and inorganic phosphate salts were separately loaded into temperature-sensitive liposome composed of 90% DPPC and 10% DMPC. When heated from the room temperature to 35- 37 ℃ , the entrapped calcium, inorganic phosphate and fl~rin released from the liposome,A mixture of Ca- Pi and F-loaded liposome formed apatite. The mineral was indicated by a rapid drop in suspension PH from 7.4 approximately 6. This was illustrated by applying a liposome suspension warmed human dentin and enamel surface which resulted in deposition of apatite onto the tissue substrates. 相似文献
38.
目的 利用聚联乙炔的刺激响应性原理,制备一种新型时间温度指示剂.方法 将聚联乙炔脂质体溶液置于不同的水浴温度下,探究其热致变色特性;采用内源乳化法将联乙炔单体形成的脂质体包裹在海藻酸钙凝胶微球中.时间温度指示剂由0.25 g脂质体凝胶微球和0.5 g的不同质量分数(20%,30%,40%,50%)吐温20溶液组成,以b*值为颜色变化参数研究其显色动力学特性.结果 聚联乙炔脂质体溶液颜色转变温度为65℃;时间温度指示剂颜色变化参数与时间呈指数关系,吐温20溶液浓度越高,温度越高,其变色速率越快,并且变色终点与温度、吐温20溶液浓度有关.结论 该款新型时间温度指示剂有较高的温度敏感性,有应用于指示食品流通过程中温度历史的潜能. 相似文献
39.
RNA interference (RNAi) is emerging as a powerful approach in cancer treatment. siRNA is an important RNAi tool that can be designed to specifically silence the expression of genes involved in drug resistance and chemotherapeutic inactivity. Combining siRNA and other therapeutic agents can overcome the multidrug resistance (MDR) phenomenon by simultaneously silencing genes and enhancing chemotherapeutic activity. Moreover, the therapeutic efficiency of anticancer drugs can be significantly improved by additive or synergistic effects induced by siRNA and combined therapies. Co-delivery of these diverse anticancer agents, however, requires specially designed nanocarriers. This review highlights the recent trends in siRNA/anticancer drug co-delivery systems under the major categories of liposomes/lipid, polymeric and inorganic nanoplatforms. The objective is to discuss the strategies for nanocarrier-based co-delivery systems using siRNA/anticancer drug combinations, emphasizing various siRNA targets that help overcome MDR and enhance therapeutic efficiency. 相似文献
40.
冻结过程对脂质体粒径影响的实验研究 总被引:2,自引:0,他引:2
为了研究不同保证剂、不同保护剂浓度在冻结过程中对脂质体粒径的影响,找到对脂质体保护效果较好的保护剂及其浓度,把1%、5%、10%、15%四种浓度的含葡萄糖、蔗糖、甘露醇、海藻糖的脂质体悬浮液按5℃/min的降温速率冻结到玻璃化转变温度Tg以下,然后在4℃冰箱中复温;分别测量脂质体冻结前、冻结后的平均粒径。冻结前以葡萄糖、蔗糖、甘露醇、海藻糖作保护剂的脂质体粒径最小的浓度分别为5%、10%、15%、10%;冻结后以葡萄糖、蔗糖、甘露醇、海藻糖作保护剂的脂质体粒径变化最小的浓度都为15%。 相似文献