Modeling the intact form of the {alpha}1-proteinase inhibitor

The structure of the intact form of the serpin SRC="http://peds.oxfordjournals.org/math/alpha.gif" ALT="{alpha}" BORDER="0">₁-proteinaseinhibitor has been modeled based on the assumption that thecentral strand s4A of the six-stranded ß-sheet A ofthe cleaved inhibitor is not incorporated into the sheet ofintact SRC="http://peds.oxfordjournals.org/math/alpha.gif" ALT="{alpha}" BORDER="0">₁-proteinase inhibitor. This strand was removed fromits position in the center of the sheet by suitable rotationsabout the backbone dihedrals of Lys343 using molecular graphics.The resulting structure was then annealed using molecular dynamics(MD) while applying progressive distance restraints to the reactivepeptide bond (Met358-Ser359) for 50 ps. During this time, thedisrupted ß-sheet reformed to create a five-strandedß-sheet with strands 3 and 5 in a parallel arrangement.This change and accompanying structural rearrangements are largelyconfirmed by the X-ray structure of plakalbumin, whose structurereflects the overall structure of intact serpins. The successfulmodeling experiment demonstrates the utility of MD for makinggross structural predictions based on related structures. Thebinding loop of the intact form is modeled to allow dockingwith serine proteinases, in particular thrombin, which mosthighly constrains the possible conformations of the bindingloop.     

Engineering of alpha1-antitrypsin variants selective for subtilisin-like proprotein convertases PACE4 and PC6: importance of the P2' residue in stable complex formation of the serpin with proprotein convertase

Furin and PACE4, members of the subtilisin-like proprotein convertase (SPC) family, have been implicated in the metastatic progression of certain tumors in addition to the activation of viral coat proteins and bacterial toxins, indicating that these enzymes are potential targets for therapeutic agents. Alpha1-Antitrypsin Portland is an engineered alpha1-antitrypsin designed as a furin-specific inhibitor and has been used as a tool in the functional analysis of furin. In this work, we engineered rat alpha1-antitrypsin to create a PACE4-specific inhibitor. Substituting Arg-Arg-Arg-Arg for Ala-Val-Pro-Met(352) at P4-P1 and Ala for Leu(354) at P2' created a potent PACE4- and PC6-specific inhibitor. This variant (RRRRSA) formed an SDS- and heat-stable serpin/proteinase complex with PACE4 or PC6 and inhibited both enzyme activities. The RRRRSA variant was efficiently cleaved by furin without formation of the stable complex. This is the first report of a highly selective protein-based inhibitor of PACE4 and PC6. This inhibitor will be useful in delineating the roles of PACE4 and PC6 localized in the extracellular matrix.     

基于DFT滤波器组的多信号高效数字下变频

高速采样数据的快速下变频是大监视带宽数字接收机研究中的一个难点问题,该文基于DFT滤波器组提出了一种多信号的高效数字下变频结构。该结构在完成高采样率下的宽带高效数字下变频和采样率变换的同时,还具备并行处理多信号的能力,其结构中单级乘法结构的采用不仅缩短了处理时间,还减少了所需的硬件资源,在面对参数变化的信号时,优势更加明显。计算机仿真和FPGA硬件仿真验证了该结构的可行性。     

某复杂SRC核心筒结构施工节点深化设计

钢骨混凝土(SRC)结构结合了钢结构和混凝土结构的优点,在高层建筑框架结构体系中广泛应用.结合实际工程,从节点处刚接和铰接方式选择、剪力墙与SRC柱的节点构造及SRC暗柱与暗梁节点构造等方面,介绍了其对结构施工的影响.进一步阐述了钢骨柱的制作流程和施工要点,以及施工中可能出现的问题和采取的相应措施.     

蜂窝状钢骨混凝土T形柱抗震性能有限元分析

利用大型有限元软件ANSYS对蜂窝状钢骨混凝土T形柱在低周反复荷载作用下的抗震性能进行分析,并考虑不同轴压力系数、剪跨比、体积配箍率、钢骨开洞率等参数对蜂窝状钢骨混凝土T形柱的影响。分析结果表明:轴压力系数和剪跨比对试件的延性有很大影响;而体积配箍率对于提高柱子的延性和承载力有一定贡献,但不显著;适当的钢骨腹板开洞率可以提高试件的延性和承载能力。     

不同类型异形柱耐震性与适用性分析

目前对普通钢筋混凝土异形柱、带暗柱钢筋混凝土异形柱、型钢混凝土异形柱的适用性研究较少。研究采用延性系数、承载力、耗能能力及极限侧移角反映异形柱的耐震性,采用含钢率反映异形柱的经济性。在此基础上,可分别用延性系数、承载力、耗能能力、极限侧移角与含钢率的比值确定不同类型异形柱的适用性。研究结果表明：当含钢率在3．00％～4...     

型钢混凝土扁梁抗剪承载力的研究分析

主要研究配箍率和型钢腹板高度对型钢混凝土扁梁抗剪承载能力的影响.通过改变扁梁的箍筋间距和型钢腹板高度,采用ANSYS有限元程序计算得到其抗剪承载力,并对计算结果进行了对比分析,从而得出这两个主要影响因素对型钢混凝土扁梁抗剪承载力的影响.     

高含钢率SRC柱轴压承载性能研究

通过对3根含钢率为24.3%的SRC柱的轴压试验,对高含钢率SRC柱构件的轴压承载性能进行了研究。分析了试件的荷载-位移关系曲线特征,揭示了高含钢率SRC柱以钢骨为主要受力部分,依次发生混凝土开裂和压溃、钢筋屈曲、钢骨弯曲失稳等破坏形态。利用有限元软件ABAQUS对试验过程进行了模拟。研究表明,高含钢率SRC柱具有钢构件的承载特性,钢骨塑性阶段的整体失稳导致极限后的承载力下降;工程设计可以采用塑性准则和叠加原理计算其极限承载力。     

宁波市鄞州金贸中心结构设计

从建筑整体分析计算等方面,介绍了宁波鄞州金贸中心大楼的结构设计。针对结构的平面不规则,介绍了在概念设计阶段对平面结构布置的处理。对不同的桩基础方案进行了对比,给出了合理的基础设计方案。针对该建筑地下室无梁楼盖不规则,且存在开洞的问题,对该部分进行了有限元分析,对高应力区域进行了加强。该建筑转换梁采用格构式钢骨混凝土结构,对该大梁进行了内力计算与分析,阐述了其设计要点及应该注意的问题。     

SRC撇油机在热轧乳液系统中的应用

本文介绍一种新型的热轧乳液过滤装置结构、功能,并阐述了这种新型过滤装置的在实践中应用的先进性。