Anti-complementary activity of enzyme-treated traditional Korean rice wine (Makgeolli) hydrolysates

BACKGROUND: Makgeolli brewed from rice contains about 150 g kg^?1 alcohol and has a fragrance as well as an acidic and sweet taste. During the brewing process, by‐products such as rice bran and brewery cake are produced. At the end of fermentation the matured mash is transferred to a filter cloth and the Makgeolli is squeezed out from the cake, leaving the lees of the mash. These by‐products have continued to increase every year, resulting in an ecological problem. It is therefore important to develop new uses for them. The objective of this study was to use the by‐products from the brewing of Makgeolli as a valuable functional food or nutraceutical. RESULTS: The anti‐complementary activities of crude polysaccharides isolated from Cytolase hydrolysates of Makgeolli lees at concentrations of 1000 and 500 µg mL^?1 were 84.15 and 78.70% respectively. The activity of polysaccharide krestin (PSK) was 60.00% at 1000 µg mL^?1. The active polysaccharide obtained with Cytolase comprised mainly glucose and mannose (molar ratio 1.00:0.62). CONCLUSION: Glucose‐ and mannose‐rich crude polysaccharides were isolated from the Cytolase hydrolysate of Makgeolli lees. The polysaccharides retain anti‐complementary activity to enhance the immune system as a functional food or nutraceutical. Copyright © 2011 Society of Chemical Industry     

UASB工艺在规模化处理米酒糟废水中的应用

米香型白酒为半固态发酵、蒸馏而成,其酒糟水具有数量大、蛋白含量高等特点,直排势必会给当地环境造成巨大的污染。采用厌氧消化-三级曝气-三级沉淀的工艺,可使处理后的酒糟废水COD达到100 mg/L以内,达标排放,同时可以产生沼气,获得一定的经济效益。着重探讨了UASB厌氧消化工艺包含的菌种驯化、菌种扩培、厌氧罐启动、厌氧罐处理能力核算、进料方式的影响等5个技术环节的要求,同时简要说明好氧处理阶段,曝气环节通气量要求、沉淀环节污泥回流要求等信息,希望能给相关行业提供积极的借鉴意义。     

复合菌生物转化白酒糟发酵条件的优化

以未发酵白酒糟培养基为对照组,探讨不同菌种组合以及发酵条件对固态发酵白酒糟中蛋白含量的影响,旨在提高酒糟中真蛋白的含量。选用枯草芽孢杆菌、白地霉和产朊假丝酵母对白酒糟进行单菌和复合菌发酵实验,结果显示三菌组合发酵效果最好。通过三菌单因素发酵试验及正交试验确定最佳发酵条件为：添加15%的麸皮和2%的尿素,先接种5%的枯草芽孢杆菌和10%的白地霉30 ℃培养24 h,然后接种5%的产朊假丝酵母30 ℃培养72 h,此时真蛋白的含量为24.85%,粗蛋白含量达32.09%,粗纤维含量降低到17.66%。     

Evolution of esters in aged Chardonnay wines obtained with different vinification methods

BACKGROUND: During wine ageing on lees yeast lysis takes place and some enzymes from the yeast cells, such as esterases, may be released. These enzymes might determine ester synthesis and could be also involved in their breakdown. In this study, the evolution of esters in Chardonnay wine fermented in stainless steel tanks and aged in bottles (conventional vinification) was compared with the evolution of esters in the same wine fermented and aged in barrel on lees. In this way, it was hoped to determine the influence of distinct methods of vinification on the evolution of esters in Chardonnay wine. RESULTS: The concentration of acetate esters of higher alcohols decreased in all the wines independently of the vinification method. However, the ethyl esters of fatty acids decreased in wine aged in barrel and increased in wine aged in bottle, so it would seem that these compounds interact with the lees present in the barrels and/or with the wood of the barrel. The ethyl esters of organic acids increased during ageing both in barrel and bottle. CONCLUSION: The ageing of Chardonnay wine in barrel on lees did not favour the formation of esters compared to the same wine aged in bottle. Copyright © 2009 Society of Chemical Industry     

酒糟大曲的生产技术及工艺探索

酒糟大曲以小麦为主要原料,以新鲜的白酒生产的副产物—酒糟为辅料,同时添加一定比例的曲粉副产物—飞箩曲;小麦经粉碎、粗细比为6∶4;新鲜酒糟添加比例占小麦的15%,飞箩曲添加比例为占小麦和酒糟总和的4%;曲坯水分含量控制在38%～40%之间,防止小麦霉变,酒糟防止生绿霉、黄霉,拌料透彻均匀,先拌料后加水;曲房要定期、不定期清洁,保持卫生;曲坯入房曲的挂衣期、晾霉以及最后出房,整个过程中密切关注温度变化,防止升温过猛,严格技术和工艺参数要求,按其生产所得的大曲周期短,糖化力高,成本低的特点。     

Biohydrogen production improvement using hot compressed water pretreatment on sake brewery waste

Most hydrogen is derived from fossil fuels. Therefore, more environmentally friendly methods for hydrogen production have been investigated. The present report describes a hot compressed water (HCW) pretreatment to increase hydrogen production using sake lees, which is an industrial waste of sake production. The inoculum is obtained from treated biogas slurry. The temperatures of HCW are 130 (0.3 MPa), 150 (0.5 MPa), and 180 °C (0.8 MPa) for 15–120 min. Gas production was analyzed using gas chromatography; fermentation liquid analyses were performed using HPLC. The modified Gompertz model was used to determine hydrogen potential, lag time, and production rate. Results show an increase in the degradation of sake lees with longer holding time and higher temperature. Total sugar and organic acids also are influenced by HCW pretreatment. The maximum hydrogen yield was obtained at 130 °C for 60 min with the result 112.07 mL H₂/g COD. The HCW pretreatment successfully decreased the lag phase of biohydrogen production and increased the degree of acidification. Clostridium butyricum, C. acetobutylicum, and other Clostridium sp. were identified in all samples, while Pantoea agglomerans was detected in two samples.     

DDGS用大型卧螺离心机的设计与应用

介绍了国产DDGS工艺用大型卧螺离心机的结构设计以及在DDGS生产工艺中的试验结果。     

以啤酒糟为主要原料生产奶牛发酵饲料菌种筛选技术研究

以啤酒糟为主要原料生产奶牛发酵饲料,通过试验确定最佳的菌种组合：金针菇A32＋酒精酵母LQ一2—11＋产朊假丝酵母LQ～2—17＋枯草芽孢杆菌LQ-1-8。     

响应面法优化玫瑰醋糟酶解工艺

针对玫瑰醋糟组织致密,高酸性的特点,从水解度的角度出发,通过采用响应面方法对玫瑰醋糟中的大米蛋白提取过程中的温度、酶用量、pH等工艺条件进行了优化.采用Plackett-Burman(PB)设计法,对不同的酶用量、固液比、温度、时间、pH值5个因素对大米蛋白水解度的影响进行评价.结果表明:温度、酶用量、pH值为米糟蛋白提取过程中的主要影响因素,用中心组合设计及响应面分析法确定主要因素的最优条件,为温度56.8℃、酶用量1.1％、pH7.9,得到大米蛋白提取液的水解度为10.04％.     

以秸秆和啤酒糟为主要原料生产奶牛发酵饲料中发酵培养基配方的研究

以秸秆和啤酒糟为主要原料生产奶牛发酵饲料,通过试验确定最终发酵培养基配方为干啤酒糟60%、玉米秸秆粉10%、豆粕粉10%、玉米浆10%、麸皮5%、尿素3%、硫酸铵2%。