Selection of RNA-Cleaving TNA Enzymes for Cellular Mg2+ Imaging

Catalytic DNA-based fluorescent sensors have enabled cellular imaging of metal ions such as Mg²⁺. However, natural DNA is prone to nuclease-mediated degradation. Here, we report the in vitro selection of threose nucleic acid enzymes (TNAzymes) with RNA endonuclease activities. One such TNAzyme, T17–22, catalyzes a site-specific RNA cleavage reaction with a k_cat of 0.017 min⁻¹ and K_M of 675 nM. A fluorescent sensor based on T17–22 responds to an increasing concentration of Mg²⁺ with a limit of detection at 0.35 mM. This TNAzyme-based sensor also allows cellular imaging of Mg²⁺. This work presents the first proof-of-concept demonstration of using a TNA catalyst in cellular metal ion imaging.     

Differences in Oxidative Stability,Sensory Properties,and Volatile Compounds of Pepper Aromatized Sunflower Oils Prepared by Different Methods during Accelerated Storage

The study aims to compare the differences in the oxidative stability, sensory properties, and volatile compounds during accelerated storage of pepper aromatized sunflower oil (PASO) samples prepared by maceration method (PASO-M), co-pressing method (PASO-C), and direct addition method (PASO-A), respectively. The results exhibit that their oxidative stability is in the relative order: PASO-A > PASO-C > PASO-M. Meanwhile, the PASO-A sample is the most preferable aromatized sunflower oil by consumers according to sensory analysis. In addition, a total of 83 volatile compounds are identified in the three PASO samples during the storage, and the principal component analysis (PCA) shows that their volatile compounds are quite different at the initial stage of the storage. Still, they are similar among each other from the middle of storage period. As consequence, the aromatized sunflower oil sample prepared by the direct-addition method during the storage possesses better oxidative stability and sensory attributes, and there is not much difference among their volatile compounds, indicating that the aromatized sunflower oil prepared by direct-addition method can be developed as promising aromatized sunflower oil. Practical applications: This study shows that aromatization increases oxidative stability and sensory properties of sunflower oil. By this way some sunflower oils with special sensory properties are obtained: special smell and taste, special color, etc. These peppers aromatized sunflower oils can be used to increase taste and smell in some dishes. For example, they can be used in some salads and some roast meats to give more taste and color.     

运用排替压力法定量预测断层侧向封闭能力——以济阳坳陷为例

断层对油气的封闭作用主要体现在侧向封闭上,而断层岩的排替压力是评价断层侧向封闭的主要参数,泥质含量和埋深则是影响岩石排替压力的主要因素.断层岩的泥质含量或埋深越大,其排替压力越大,对油气的封堵能力越强.通过实验测试手段可以建立不同泥质含量断层岩的排替压力与埋深的关系,从而可以实现对断层岩排替压力的预测.断层岩与储层的排...     

植物功能性食品在促进健康和预防疾病中的作用

对一些常见的植物功能性食物在疾病预防和促进健康中的作用及其功能性因子的研究现状进行了综述。