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1.
An engineered variant of the yeast Pichia pastoris was used to produce the potential malaria vaccine D1M1. This paper describes the implementation of an expanded bed adsorption chromatography step into a sequential cultivation process of the yeast in order to link protein expression, cell release, and product capture of the secreted protein in a fully automated plant. The process is operated with a semi-continuous strategy for an integrated production. Process improvements have been achieved using design of experiments. Resulting purities of product achieved up to 87% with recoveries of 51% in a single downstream operation. Furthermore, a multivariate analysis of historical batch data was used to develop a golden batch model for process monitoring. 相似文献
2.
Ignatius Y. Chan 《Microscopy research and technique》1987,5(2):181-188
A new transmission electron microscopy (TEM) specimen preparation procedure for high temperature experiments using a controlled atmosphere specimen holder (HTCASH) has been developed. It is designed for studying the microstructure of catalyst specimens before and after treatments in various gases. The procedure involved (1) finding a new formula for the embedding material, (2) devising a new method of making specimen supports, and (3) developing a method for removing the embedding material after the specimen has been microtomed. These techniques were then brought together to produce the ideal specimens for the HTCASH experiments. As an extra benefit, this procedure is also suitable for preparing specimens for ultrahigh resolution imaging experiments. The application of the new procedure in HTCASH experiments is illustrated through a high temperature reduction of a Co/SiO2-923 catalyst. 相似文献
3.
A probabilistic model for cleavage fracture with a length scale--parameter estimation and predictions of stationary crack experiments 总被引:1,自引:0,他引:1
This study presents a large experimental investigation in the transition temperature region on a modified A508 steel. Tests were carried out on single-edge-notch-bend specimens with three different crack depth over specimen width ratios to capture the strong constraint effect on fracture toughness. Three test temperatures were considered, covering a range of 85 °C. All specimens failed by cleavage fracture prior to ductile tearing. A recently proposed probabilistic model for the cumulative failure by cleavage was applied to the comprehensive sets of experimental data. This modified weakest link model incorporates a length scale, which together with a threshold stress reduce the scatter in predicted toughness distributions as well as introduces a fracture toughness threshold value. Model parameters were estimated by a robust procedure, which is crucial in applications of probabilistic models to real structures. The conformity between predicted and experimental toughness distributions, respectively, were notable at all the test temperatures. 相似文献
4.
In air-compression systems, instabilities occur during operation close to their peak pressure-rise capability. However, the
peak efficiency of a compression system lies close to this region of instability. A surge is a violent mode of instability
where there is total breakdown of flow in the system and pressure-rise capability is lost drastically. Generally, all compression
systems operate with a margin defined as the ‘surge margin’, and, consequently, system operational efficiency is lower. It
is of interest to study compression-system surge to understand its dynamics in order to operate compression systems close
to the instability for achieving high efficiency safely without encountering surge. Unsteady pressure data from a compression
system, captured during surge oscillations, reveal many aspects of flow physics and are analysed to understand the surge dynamics
of the system. A set of controlled experiments was conducted with a simple desktop experimental test set-up and essential
aspects of surge dynamics have been characterised. 相似文献
5.
The Error-in-Variables Model (EVM) applies to a large class of problems in parameter estimation in which there is error in all the variables. This property makes it of more general applicability than the widely used technique of ordinary Least Squares. While there is a voluminous literature on EVM there is nothing on a general method for designing experiments when EVM applies. In this paper we present an extension of the concept of D-optimality to provide such a general method. It is illustrated by applying it to the estimation of copolymer reactivity ratios and to fitting an ellipse. 相似文献
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Complementary modelling of fluid separation processes 总被引:1,自引:0,他引:1
Optimal functioning of numerous technological processes depends primarily on relevant process design, properly selected column internals and sufficient understanding of the process behaviour. This can be achieved only with the help of accurate and reliable process models capable of considering process rates in a rigorous way, with respect to both transport phenomena and chemistry. In this article, a new modelling concept called complementary modelling is suggested for a large class of fluid separation processes. Since the conditions and criteria for these processes vary considerably, it is impossible to develop a unified modelling approach. Instead, a reasonable and effective combination of different modelling approaches provides solutions to many present and future tasks. The complementary modelling is discussed in detail and illustrated with several case studies. 相似文献
9.
Simultaneous solving of balancing and sequencing problems with station-dependent assembly times for mixed-model assembly lines 总被引:1,自引:0,他引:1
In spite of many studies, investigating balancing and sequencing problems in Mixed-Model Assembly Line (MMAL) individually, this paper solves them simultaneously aiming to minimize total utility work. A new Mixed-Integer Linear Programming (MILP) model is developed to provide the exact solution of the problem with station-dependent assembly times. Because of NP-hardness, a Simulated Annealing (SA) is applied and compared to the Co-evolutionary Genetic Algorithm (Co-GA) from the literature. To strengthen the search process, two main hypotheses, namely simultaneous search and feasible search, are developed contrasting Co-GA. Various parameters of SA are reviewed to calibrate the algorithm by means of Taguchi design of experiments. Numerical results statistically show the efficiency and effectiveness of the proposed SA in terms of both the quality of solution and the time of achieving the best solution. Finally, the contribution of each hypothesis in this superiority is analyzed. 相似文献
10.