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1.
Coxsackievirus A16 (CA16) is one of the major causative agents of hand, foot, and mouth disease (HFMD). Children aged <5 years are the most affected by CA16 HFMD globally. Although clinical symptoms of CA16 infections are usually mild, severe complications, such as aseptic meningitis or even death, have been recorded. Currently, no vaccine or antiviral therapy for CA16 infection exists. Single-chain variable fragment (scFv) antibodies significantly inhibit viral infection and could be a potential treatment for controlling the infection. In this study, scFv phage display libraries were constructed from splenocytes of a laying hen immunized with CA16-infected lysate. The pComb3X vector containing the scFv genes was introduced into ER2738 Escherichia coli and rescued by helper phages to express scFv molecules. After screening with five cycles of bio-panning, an effective scFv antibody showing favorable binding activity to proteins in CA16-infected lysate on ELISA plates was selected. Importantly, the selected scFv clone showed a neutralizing capability against the CA16 virus and cross-reacted with viral proteins in EV71-infected lysate. Intriguingly, polyclonal IgY antibody not only showed binding specificity against proteins in CA16-infected lysate but also showed significant neutralization activities. Nevertheless, IgY-binding protein did not cross-react with proteins in EV71-infected lysate. These results suggest that the IgY- and scFv-binding protein antibodies provide protection against CA16 viral infection in in vitro assays and may be potential candidates for treating CA16 infection in vulnerable young children.  相似文献   
2.
简述了矿用蓄电池机车控制器的发展历程以及机车逆变器的工作原理,分析现有机车逆变器所存在的电磁干扰、参数显示及机车司控室空间占用问题。针对以上问题进行了优化设计,改进了逆变器的电磁兼容性,改善了机车运行参数显示结构,集成了甲烷断电仪主机功能。通过对比分析可知,优化设计可有效减轻或抑制设备谐波干扰,增加了设备运行的稳定性,提高了现场安全操作性。  相似文献   
3.
为实现国产自主可控,基于国产CPU 和麒麟操作系统,采用Qt 元语言(qt meta language,QML)和图形 处理器(graphics processing unit,GPU)编程的模式设计一种跨平台的实时显示软件。详细阐述基于QML 的软件架构 设计,并对软件设计中的QML、C++及GPU 的通信模式、QML 下CPU+GPU 编程实时显示性能优化方法以及QML 显示控件设计等关键技术进行说明,验证其应用。结果证明:该模型具有可靠、稳定、高效和跨平台等特点,满足 实时显示应用要求。  相似文献   
4.
为建立高效的西马特罗(Cimaterol, CIM)检测方法,试验用以重氮化法合成的西马特罗完全抗原免疫新西兰大白兔,提取兔脾细胞总RNA,反转录合成cDNA第一链,设计兼并引物,扩增兔源抗体重链可变区(VH)和轻链可变区(VL),通过45 bp的连接肽(Linker)连接成VH-Linker-VL并大量扩增;利用噬菌体表面展示技术将单链抗体(ScFv)片段与噬菌体展示载体pCANTAB-5e连接,电转化至大肠杆菌TG1感受态细胞,得到兔源噬菌体ScFv抗体库。通过聚丙烯酰胺凝胶电泳(SDS-PAGE)判断是否偶联成功,用紫外扫描(UV)及基质辅助激光解析串联飞行时间质谱(MALDI-TOF-MS)测定人工抗原的偶联比。将富集的抗体库侵染对数期大肠杆菌进行抗体库滴度测定合成抗体库的质量进行鉴定,对抗体库进行三轮淘选,取最优的CIM-ScFv进行氨基酸序列分析。结果表明:CIM-BSA及CIM-OVA偶联比分别为8:1及10:1,CIM-BSA免疫原免疫新西兰大白兔,间接ELISA测得免疫血清校价为1:32000,免疫血清半数抑制浓度为9.77 ng/mL, 曲线的拟合度为0.997。抗西马特罗兔源噬菌体ScFv免疫库的插入率为92%,库容为1×1010 pfu,多样性为83.3%,经过4轮淘选,获得一株与CIM有结合活性的噬菌体ScFv菌。以上结果表明西马特罗完全抗原的合成效果良好,可用于建立西马特罗检测方法,兔源噬菌体抗体库为快速获得CIM单链抗体奠定基础,为西马特罗快速检测提供新思路。  相似文献   
5.
视觉显示终端(visual display terminal,VDT)和纸质文稿共同成为人们生活和学习中不可或缺的阅读媒介,提供适宜的VDT与纸质交互阅读照明环境是提升室内照明环境质量、满足人民健康需求的重要发展方向。为探讨照明环境对交互阅读的视觉和认知的影响,采用主观评价、视觉任务绩效和生理指标测量相结合的方法,开展照明环境主客观评价实验。搭建了交互阅读照明环境实验平台,研究了水平照度、照度配比、色温、屏幕亮度4项照明参数对交互阅读视觉舒适、视觉功效、视觉疲劳3种视觉感知,认知效率和情绪唤醒2种认知表现的影响。实验结果显示,水平照度对视觉疲劳影响最大;照度配比主效应对交互阅读的视觉感知和认知表现均有显著影响;光源色温对交互阅读视觉舒适和视觉功效影响最大;VDT屏幕亮度对交互阅读视觉疲劳、认知效率和情绪唤醒影响最大。根据各相关要素对评价指标的影响分析,得出高视觉舒适且高情绪唤醒的交互阅读最佳照明环境参数为水平照度750 lx、垂直照度300 lx(照度配比2.5)、色温4 000 K,最佳屏幕亮度为100 cd/m2;高视觉功效以及高认知效率、低视觉疲劳的交互阅读最佳照明环境参数为:水平照度300 lx、垂直照度200 lx(照度配比1.5)、色温5 000 K,且屏幕亮度建议为100 cd/m2。研究结果为交互阅读的照明设计和优化提供依据。  相似文献   
6.
Thermally robust and highly efficient green-emitting luminescent ceramics are gradually attracting great attention as promising phosphors using in high-brightness laser phosphor display to reduce serious speckle noise as well as high cost. However, lumen density is still seriously restricting their potential applications especially under high-power density laser due to insufficient absorption of blue laser and significant thermal quenching. Here, we report an Al2O3-LuAG: Ce composite ceramic phosphor (CCP) for high-brightness laser phosphor display. Owing to good optical properties and high thermal conductivity of Al2O3, the Al2O3-LuAG: Ce CCP shows high photoluminescence quantum yield (79.6%), low thermal quenching (only 3.2% loss in luminescence at 200°C), and high thermal conductivity (18.9 W·m−1·K−1). Moreover, the Al2O3, as scattering centers, enhances the Rayleigh–Mie scattering of the blue laser, and hence the absorption of the Al2O3-LuAG: Ce CCP exhibits a remarkable improvement (~2.3 times) at 450 nm. Finally, with optimized thickness (0.3 mm) of Al2O3-LuAG: Ce CCP, an excellent luminous efficiency (216 lm·W−1) and outstanding lumen density (6129 lm·mm−2) of the green-emitting light source was obtained by driving under a high-power density (28.33 W·mm−2) blue laser. All of those validate the suitability of the Al2O3-LuAG: Ce CCP for high-brightness display.  相似文献   
7.
Conformationally constrained peptides hold promise as molecular tools in chemical biology and as a new modality in drug discovery. The construction and screening of a target-focused library could be a promising approach for the generation of de novo ligands or inhibitors against target proteins. Here, we have prepared a protein kinase-focused library by chemically modifying helix-loop-helix (HLH) peptides displayed on phage and subsequently tethered to adenosine. The library was screened against aurora kinase A (AurA). The selected HLH peptide Bip - 3 retained the α-helical structure and bound to AurA with a KD value of 13.7 μM. Bip - 3 and the adenosine-tethered peptide Bip - 3 - Adc provided IC50 values of 103 μM and 7.7 μM, respectively, suggesting that Bip - 3 - Adc bivalently inhibited AurA. In addition, the selectivity of Bip - 3 - Adc to several protein kinases was tested, and was highest against AurA. These results demonstrate that chemical modification can enable the construction of a kinase-focused library of phage-displayed HLH peptides.  相似文献   
8.
以AT89C52单片机作为微控制器,设计了一个智能温度测量系统。该系统通过DS18B20温度传感器实现环境温度的采集和A/D转换,模数转化后的电信号送入AT89C52单片机,再通过单片机将其送入LCD1602数码管进行显示。通过实验结果可以看出,相比较传统的温度采集方法,该系统能够快速准确地检测温度,并且检测精度可以达到0.1 ;同时,该系统具有过温点设定值调节及温度上下限蜂鸣提示的优点。  相似文献   
9.
This study was conducted to investigate the virtual display effects on direct interaction performance metrics such as accuracy, task completion time, and comfort. Eighteen participants performed tapping (pointing) tasks in the coronal plane by directly reaching for tapping targets at three egocentric distance levels, with three indices of difficulty at each egocentric distance. The position data and severity of cybersickness symptoms were collected with a motion system and a symptom questionnaire, respectively. The results indicated that accuracy was higher with the stereoscopic widescreen display than with the head mounted display. However, no significant differences in task completion time, throughput, and cybersickness were observed between the two VR displays. In addition, increasing the egocentric distance improved accuracy and lengthened the task completion time, whereas increasing the task difficulty lengthened the task completion time but did not affect the accuracy. The findings are important and informative for users in choosing between the two virtual reality displays. Generally, the stereoscopic widescreen display can be recommended for tasks requiring high egocentric distance accuracy in the coronal plane. Furthermore, developers may refer to these findings in designing interfaces that allow a more natural way of interaction for users.  相似文献   
10.
Many therapeutic peptides can still be improved with respect to target specificity, target affinity, resistance to peptidases/proteases, physical stability, and capacity to pass through membranes required for oral delivery. Several modifications can improve the peptides’ properties, in particular those that impose (a) conformational constraint(s). Screening of constrained peptides and the identification of hits is greatly facilitated by the generation of genetically encoded libraries. Recent breakthrough bacterial, phage, and yeast display screening systems of ribosomally synthesized post-translationally constrained peptides, particularly those of lanthipeptides, are earning special attention. Here we provide an overview of display systems for constrained, genetically encoded peptides and indicate prospects of constrained peptide-displaying phage and bacterial systems as such in vivo.  相似文献   
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