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1.
《Journal of dairy science》2022,105(6):5471-5492
The detection of reproductive tract disease (RTD) 3 wk postpartum is important because of its effect on subsequent reproductive outcomes. Numerous methods for the diagnosis of RTD are described, some of which are more practical and instantaneous in terms of diagnosis. Two of these methods involve identification of purulent vaginal discharge (PVD) and evidence of ultrasonographic uterine changes indicative of endometritis (UE). The objectives of our retrospective observational study were (1) to assess the association of PVD or UE score at the prebreeding examination (PBE) with the hazard of pregnancy within the subsequent breeding season; (2) to determine the test sensitivity (Se) and specificity (Sp) at the point of sampling of both tests using a Bayesian latent class model; and (3) to determine the effect of varying positivity thresholds on test accuracy. To achieve these objectives, we analyzed an initial data set of 5,049 PBE from 2,460 spring-calved cows in 8 herds between 2014 and 2018. Each PBE was conducted once between 25 and 86 d in milk. At each PBE, vaginal discharge was obtained with a Metricheck device (Simcro) whereas uterine contents were assessed using transrectal ultrasonography. Purulent vaginal discharge was scored on a scale of 0 to 3 depending on discharge character, and UE was scored on a scale of 0 to 4 depending on the presence and consistency of intraluminal fluid. Cows with scores of ≥2 in either test had received treatment. Fertility data were available from 4,756 PBE after data exclusion. The association between PVD or UE score at the PBE and subsequent hazard of pregnancy was analyzed using a Cox proportional hazards model. Cows with a PVD score of 2 or 3 were less likely to conceive than cows with a PVD score 0 [score 2 hazard ratio (HR) = 0.74; 95% confidence interval (CI): 0.59–0.94; score 3 HR = 0.65; 95% CI: 0.51–0.84]. Cows with a UE score of 1, 2, 3, or 4 were less likely to conceive than cows with a UE score of 0 (score 1 HR = 0.82; 95% CI: 0.73–0.93; score 2 HR = 0.79; 95% CI: 0.62–1.00; score 3 HR = 0.43; 95% CI: 0.43–0.90; score 4 HR = 0.39; 95% CI: 0.26–0.58). To determine the Se and Sp of PVD or UE score for diagnosis of RTD at the time of PBE, a Bayesian latent class model was fitted on 2,460 individual cow PBE. Flat priors were used for the Se and Sp of UE, whereas informative priors were used for PVD Se (mode = 65%, 5th percentile = 45%) and Sp (mode = 90%, 5th percentile = 80%) and RTD prevalence (mode = 20%, 5th percentile = 10%). Posterior estimates (median and 95% Bayesian probability intervals; BPI) were obtained using ‘rjags' (R Studio). The optimal test thresholds (PVD and UE score ≥1) were selected by assessing the effect of different thresholds on test estimates and using a misclassification cost analysis. Based on these, median (95% BPI) Se for PVD and UE score ≥1 were 44% (29–60%) and 67% (33–100%), respectively. Median Sp for PVD and UE score ≥1 were 90% (86–93%) and 91% (86–93%), respectively. Higher scores in both tests were associated with impaired fertility, and UE scoring with a threshold of ≥1 had the highest test Se and Sp estimates although test Se was conditional on days in milk when the PBE occurred. 相似文献
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《Ceramics International》2022,48(20):30393-30406
Plasma methods are efficient processing for metal recovery from metal scrap, bearing minerals, electronic waste, etc. In this work, pure titanium nitride nanoparticles (TiN NPs) were synthesized from titanium scraps by the thermal plasma arc discharge (TPAD) method. TPAD synthesized TiN NPs have a highly crystalline nature with cubic and spherical morphologies with average particle sizes of 30–100 nm. Further, prepared TiN NPs involving surface modification (SM) or etching processes were investigated by using the non-thermal DC glow discharge plasma technique with air atmosphere at different processing times. SM@TiN NPs have a comparatively low crystalline, which was confirmed from the powder X-ray diffraction technique. SM@TiN NPs have very interesting core shell morphologies, which are due to the surface interactions of ionized air molecules. TiN and SM@TiN NPs have room-temperature ferromagnetic properties with high saturation magnetization (Ms) up to 2.6 and 3.0 emu/g and very high coercivity (Hc) of 235.5 Oe, respectively. TiN and SM@TiN NPs have superior energy storage performance with an outstanding specific capacitance of 192.8 and 435.1 F/g at a current density of 2 A/g with pseudocapacitive behavior. These results reveal that TiN and SM@TiN NPs have highly promising electrodes for supercapacitor applications. 相似文献
5.
益生菌可在肠道定植从而发挥抗炎或抗氧化活性,有利于宿主肠道健康。本实验研究了从新疆传统发酵乳制品中分离得到的8?株植物乳杆菌对大肠杆菌侵袭和过氧化氢刺激肠上皮细胞HT-29的保护作用。结果表明:在8?株植物乳杆菌中,植物乳杆菌35具有最高的黏附能力。植物乳杆菌35可通过取代、竞争、排阻的方式抑制大肠杆菌对HT-29细胞的黏附,抑制率分别为42.60%、59.17%、60.19%。植物乳杆菌35及其多糖可抑制大肠杆菌刺激HT-29细胞产生白细胞介素-8;同时保护HT-29细胞免受过氧化氢的损伤,增加超氧化物歧化酶、谷胱甘肽过氧化物酶活力水平并降低丙二醛含量。结论:植物乳杆菌35及其粗胞外多糖具有抑制大肠杆菌O157诱导的炎症性肠病的潜力。 相似文献
6.
《International Journal of Hydrogen Energy》2022,47(38):17056-17068
The study of steels which guarantee safety and reliability throughout their service life in hydrogen-rich environments has increased considerably in recent years. Their mechanical behavior in terms of hydrogen embrittlement is of utmost importance. This work aims to assess the effects of hydrogen on the tensile properties of quenched and tempered 42CrMo4 steels. Tensile tests were performed on smooth and notched specimens under different conditions: pre-charged in high pressure hydrogen gas, electrochemically pre-charged, and in-situ hydrogen charged in an acid aqueous medium. The influence of the charging methodology on the corresponding embrittlement indexes was assessed. The role of other test variables, such as the applied current density, the electrolyte composition, and the displacement rate was also studied. An important reduction of the strength was detected when notched specimens were subjected to in-situ charging. When the same tests were performed on smooth tensile specimens, the deformation results were reduced. This behavior is related to significant changes in the operative failure micromechanisms, from ductile (microvoids coalescence) in absence of hydrogen or under low hydrogen contents, to brittle (decohesion of martensite lath interfaces) under the most stringent conditions. 相似文献
7.
目的:探讨姜黄素的主要肠道代谢物四氢姜黄素(tetrahydrocurcumin,THC)对血小板活化和聚集的影响及其可能的分子机制。方法:在体外实验中,用不同浓度的THC(0、0.5、1、10 μmol/L)提前与健康人纯化血小板共同孵育40 min,然后加入凝血酶激活血小板2 min,用流式细胞术测定血小板表面CD62P和CD63的表达量,用酶联免疫吸附法测定血小板释放血小板因子-4(platelet factor-4,PF4)和趋化因子配体-5(chemokine ligand 5,CCL5)水平,用血小板聚集仪检测血小板释放ATP水平和血小板最大聚集率,用Western blot蛋白免疫印迹法检测血小板磷酸肌醇-3-激酶(phosphoinositide 3-kinase,PI3K)和Akt蛋白的磷酸化水平。结果:与模型组(血小板悬液中加入0.05%二甲基亚砜)相比,THC能抑制凝血酶诱导的血小板表面CD62P和CD63的表达,抑制PF4、CCL5和ATP的释放,降低血小板最大聚集率,下调PI3K和Akt蛋白的磷酸化水平,且呈浓度依赖效应,其中10 μmol/L的浓度下作用效果显著(P<0.01、P<0.001)。PI3K的特异性激动剂740 Y-P可部分逆转THC对PF4和CCL5释放和血小板聚集的抑制作用(P<0.05、P<0.01)。结论:THC具有显著抑制血小板活化和聚集的作用,其机制可能是THC可下调PI3K/Akt介导的信号通路。 相似文献
8.
超稳光纤链路这个概念包含超稳频率光源和超稳频率传递的光纤链路。从当前看,如何利用已有庞大的公用电信网、专用网的光纤和光网络的资源,选择一个通用的光纤通路改造成超稳光纤链路来实现频率传递,取代基于卫星的频率传递,提高传输的频率精度,这是一个巨大的系统工程。本文对超稳频率光源和超稳频率传递的光纤链路的关键技术进行研究和讨论。 相似文献
9.
《International Journal of Hydrogen Energy》2022,47(57):23909-23918
The hydrogel electrolyte is an important part of safety and development potential in zinc-based energy storage equipment due to its inherent low mechanical strength and voltage decomposition. However, hydrogel electrolytes possess a reduced working life for zinc dendrites growth and a narrow voltage window. In this study, a hydrogel electrolyte prepares by the zwitterionic monomer [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl) (MS) and sodium alginate (SA) alleviate these problems. The zwitterionic double-network hydrogel has good mechanical strength, inhibits the growth of zinc dendrites, enhances practicability, greatly increases the voltage window (0–2.4 V), and has self-healing properties to its rich functional groups. The assembled zinc-ion hybrid supercapacitors (ZHSs) have a high-power density of 172.33 W kg?1 and an energy density of 88.56 Wh·kg?1 at 0.5 A g?1. The assembled zinc-ion battery also has good electrochemical performance. Flexible ZHSs and batteries provide power to the timer stably under different bending angles. The zwitterionic double-network hydrogel can be applied to both zinc-based supercapacitors and batteries. 相似文献
10.
《Geotextiles and Geomembranes》2022,50(3):535-544
Soil column is often investigated in the improvement of dredged slurries. Different from the smear zone, the soil column forms gradually and has extremely low permeability. This study presents an analytical solution for soil consolidation considering the increasing radius of the soil column and time-dependent discharge capacity. Based on the solution, the influence of the radius' increase on the consolidation behavior is found significant when the soil column has low permeability and large final radius, and the increase of formation time can lead to the increase of consolidation speed and final consolidation degree. 相似文献