Modelling and kinetic aspects of a BTEX contaminated air-treating biofilter

In this study, the overall performance of a biofilter was evaluated in terms of its elimination capacity by using 3-D mesh techniques. The overall results indicate that the agreement between experimental data and model predictions is excellent for benzene, toluene, ethylbenzene and o-xylene (BTEX). In this study, the maximum removal rate (r _max) values for BTEX were 0.0117, 0.0126, 0.0081 and 0.0146 g m^–3 h^–1, and the half-saturation constant (K_S ) values were calculated to be 0.269, 0.297, 0.156 and 0.394 g m^–3, respectively. For this system, the coefficients of determination (r ²) of BTEX compounds were greater than 0.97. The BTEX concentration profiles along the depth were also determined using a convection–diffusion reactor (CDR) model. The sums of squares of the errors (SSEs) of BTEX were 0.0078, 0.0059, 0.0129 and 0.0269, respectively, with r ² values greater than 0.99 for all four compounds at low concentrations.     

Isolation of recombinant antibody fragments (scFv) by phage display technology for detection of almond allergens in food products

Tree nut allergies are considered an important health issue in developed countries. To comply with the regulations on food labeling, reliable allergen detection methods are required. In this work we isolated almond-specific recombinant antibody fragments (scFv) from a commercial phage display library bypassing the use of live animals, hence being consistent with the latest policies on animal welfare. To this end an iterative selection procedure employing the Tomlinson I phage display library and a crude almond protein extract was carried out. Two different almond-specific scFv (named PD1F6 and PD2C9) were isolated after two rounds of biopanning, and an indirect phage ELISA was implemented to detect the presence of almond protein in foodstuffs. The isolated scFvs demonstrated to be highly specific and allowed detection of 40 ng mL⁻¹ and 100 ng mL⁻¹ of raw and roasted almond protein, respectively. The practical detection limit of the assay in almond spiked food products was 0.1 mg g⁻¹ (110–120 ppm). The developed indirect phage ELISA was validated by analysis of 92 commercial food products, showing good correlation with the results obtained by a previously developed real-time PCR method for the detection of almond in foodstuffs. The selected phage clones can be affinity maturated to improve their sensitivity and genetically engineered to be employed in different assay formats.     

CDMA20001x EVDO网络MM4+协议监测的实现

首先对CDMA2000 1x EVDO网络中的增值业务进行了介绍,然后针对增值业务中不同网络间的多媒体消息互通问题,对关键接口MM4接口中的MM4+协议进行了介绍与监测.最后,借助哈希索引算法提出了一种新的CDR存储方案,并通过现网数据测试,验证了相关方法的可行性.     

ABC transporters Cdr1p, Cdr2p and Cdr3p of a human pathogen Candida albicans are general phospholipid translocators

We have used fluorescent 7-nitrobenz-2-oxa-1,3-diazol-4-yl (NBD)-tagged phospholipid analogues, NBD-PE (phosphatidylethanolamine), NBD-PC (phosphatidylcholine) and NBD-PS (phosphatidylserine), to demonstrate that Cdr1p and its other homologues, Cdr2p and Cdr3p, belonging to the ATP-binding cassette (ABC) superfamily behave as general phospholipid translocators. Interestingly, CDR1 and CDR2, whose overexpression leads to azole resistance in C. albicans, elicit in-to-out transbilayer phospholipid movement, while CDR3, which is not involved in drug resistance, carries out-to-in translocation of phospholipids between the two monolayers of plasma membrane. Cdr1p, Cdr2p and Cdr3p could be further distinguished on the basis of their sensitivities to different inhibitors. For example, the in-to-out activity associated with Cdr1p and Cdr2p is energy-dependent and sensitive to sulphydryl blocking agents such as N-ethylmaleimide (NEM) and cytoskeleton disrupting agent cytochalasin E, while Cdr3p-associated out-to-in activity is energy-dependent but insensitive to NEM and cytochalasin E. We found that certain drugs, such as fluconazole, cycloheximide and miconazole, to which Cdr1p confers resistance could also affect in-to-out transbilayer movement of NBD-PE, while the same drugs had no effect on Cdr3p-mediated out-to-in translocation of NBD-PE. The ineffectiveness of these drugs to affect Cdr3p mediated out-to-in phospholipid translocation further confirms the inherent difference in the directionality of phospholipid translocation between these pumps. Notwithstanding the role of some of the Cdrps in drug resistance, this study clearly demonstrates that these ABC transporters of C. albicans are phospholipid translocators and this function could represent one of the physiological functions of such large family of proteins.     

预付费及后付费融合的业务支撑系统探讨

针对预付费及后付费业务融合如何实现，提出并分析了融合支撑系统的组成、功能结构、流程，对融合支撑中涉及到的重要问题和对现有系统的影响进行了探讨、分析并提出了建议.     

千兆以太网中的字节对准

千兆以太网的数据传输采用串行方式，在接收端将进行串并转换，所以数据的字节对准的问题就随之而来。本文提出并设计了一种适合于千兆以太网的字节对准电路，来解决高速数据传输过程中的字节对准问题。     

半色调可视加密算法的综合评价算法

已有半色调可视加密(HVC)算法的评价普遍采用正确解码率(CDR)或比特错误率(BER),未考虑信息隐藏对载体图像造成的干扰。提出一种半色调可视加密算法的综合评价算法,综合考虑信息隐藏对载体图像造成的干扰和提取秘密图像的错误率,选择平均每像素干扰值(ADPP)衡量信息隐藏对载体图像造成的干扰,为保证ADPP和BER数据范围的一致性,采用秘密图像结合隐藏阈值T计算最大平均干扰值(MAD),将ADPP和BER结合MAD和系数λ综合计算,得到半色调可视加密算法的综合干扰值(IDHVC)。该算法与已有综合评价算法相比,在运算量方面具有明显优势。通过实验验证,该算法可应用于灰度和彩色半色调可视加密算法的综合评价。     

Analysis of tourist classification from cellular network data

We present and evaluate a classification method to estimate tourist presence in an area from cellular network data. Our approach is based on setting up a classifier to label users according to five main classes: residents, commuters, people in-transit, tourists and excursionists. We experiment the approach in some important tourist cities in Italy: Venice, Florence, Turin and Lecce. In the lack of sound groundtruth data, we analysed the composition of different classes obtaining results in line with domain knowledge. Moreover, these results are also supported by an analysis of the locations frequented by the tourists that well conforms with expectations. Finally, the number of users classified as tourists is strongly correlated with official statistics on tourist presence in the area.     

A small fully digital open‐loop clock and data recovery circuit for wired BANs

This paper proposes a new open‐loop and low complexity (small size) fast‐lock synchronization circuit for clock and data recovery in wearable systems. The system includes sensors embedded in textile and connected by conductive yarns. Synchronization is based on the open‐loop selection of the correct phase of the receiver clock synchronously with the incoming signal. The clock generator of the receiver is an autonomous oscillator set to operate at the same nominal frequency. The circuit lock time is at most one clock cycle, faster than all methods based on phase‐locked loops or delay‐locked loops. The circuit can be used for baseband communication independently of the signal coding method used in the physical layer, making it suitable for many applications. The fully digital circuit (including non‐return‐to‐zero inverted decoder) occupies 0.0022 in a 0.35 complementary metal‐oxide semiconductor (CMOS) process, a smaller implementation than many existing circuits, and supports a maximum system clock frequency of 70 for a 35‐data rate. Experimental results demonstrate that the proposed circuit robustly generates a synchronous clock for data recovery. The circuit is suitable for systems that tolerate some jitter but requires fast lock time, small size, and low energy consumption. Copyright © 2015 John Wiley & Sons, Ltd.     

新型倍流整流方式零电压开关PWM全桥三电平直流变换器

通过在基本的倍流整流方式零电压开关PWM全桥三电平直流变换器的一次侧绕组中串联一个阻断电容,提出一种新型变换器．该变换器利用阻断电容的电压使一次侧电流在零状态时快速下降,不必要求变压器漏感极小;输出整流管能够自然换流,避免了反向恢复造成的电压振荡和电压尖峰．并分析变换器的工作原理,通过仿真实验验证该变换器的可行性．