Understanding the metabolic burden of recombinant antibody production in Saccharomyces cerevisiae using a quantitative metabolomics approach

The cellular changes induced by heterologous protein expression in the yeast Saccharomyces cerevisiae have been analysed on many levels and found to be significant. However, even though high‐level protein production poses a metabolic burden, evaluation of the expression host at the level of the metabolome has often been neglected. We present a comparison of metabolite profiles of a wild‐type strain with those of three strains producing recombinant antibody variants of increasing size and complexity: an scFv fragment, an scFv–Fc fusion protein and a full‐length IgG molecule. Under producing conditions, all three recombinant strains showed a clear decrease in growth rate compared with the wild‐type strain and the severity of the growth phenotype increased with size of the protein. The levels of 76 intracellular metabolites were determined using a targeted (semi) quantitative mass spectrometry based approach. Based on unsupervised and supervised multivariate analysis of metabolite profiles, together with pathway activity profiling, the recombinant strains were found to be significantly different from each other and from the wild‐type strain. We observed the most prominent changes in metabolite levels for metabolites involved in amino acid and redox metabolism. Induction of the unfolded protein response was detected in all producing strains and is considered to be a contributing factor to the overall metabolic burden on the cells.     

基于光纤-无线网络的协同计算卸载算法

随着无源光网络的发展,光纤-无线网络能同时支持集中式云和边缘云计算技术,成为一种具有发展前景的网络结构。但是,现有的基于光纤-无线网络的任务协同计算卸载研究主要以最小化移动设备的能耗为目标,忽略了实时性高的任务的需求。针对实时性高的任务,提出了以最小化任务的总处理时间为目标的集中式云和边缘云协同计算卸载问题,并对其进行形式化描述。同时,通过将该问题归约为装箱问题,从而证明其为NP难解问题。提出一个启发式协同计算卸载算法,该算法通过比较不同卸载策略的任务处理时间,优先选择时间最短的任务卸载策略。同时,提出一个定制的遗传算法,获得一个更优的任务卸载策略。实验结果表明,与现有的算法相比,本文提出的启发式算法得到的任务卸载策略平均减少4.34%的任务总处理时间,而定制的遗传算法的卸载策略平均减少18.41%的任务总处理时间。同时,定制的遗传算法的卸载策略与本文提出的启发式算法相比平均减少14.49%的任务总处理时间。     

钢筋混凝土拆除爆破炮孔装药量的确定

本文从分析钢筋混凝土爆破破坏形态出发,对钢筋混凝土的配筋情况,如强度、配筋网规格、钢筋层数等对拆除爆破的影响作了量化处理,在前人的基础上大胆提出了钢筋混凝土加强抛掷爆破的炮孔装药量计算公式,并在实际工程中得到了验证。     

Perceived density,a further attribute of color,revealed by the colors favored in Japan

Colors can be characterized by three main attributes: hue, value, and saturation. But colors also exhibit other phenomenological qualities. In this study, we identify one such secondary attribute of color: perceived density. We discuss the prevalence of dense colors in Japan starting from the “48 Teas and 100 Mice” colors of the Edo period, and develop the concept of perceived density through this aspect of Japanese color preference. When vivid colors were forbidden to commoners during the Edo period, subtle variations of brownish and grayish colors were created. These colors with base tones were not salient, yet they looked dense. Muted colors with paradoxical richness are still common in Japan today. Japanese commodity design often uses muddy colors with white or gray undertones, and deep colors with black undertones. Together they form distinct groupings of relatively dense color. The perceived density of color corresponds to how dense and filled, or thin and airy a color appears. Colors of higher perceived density appear to be more packed and to have mass. Perceived density of color is unusual in that it does not have a monotonic relationship with one of the primary perceptual attributes. High apparent density is observed in a central region of an equi‐hue plane where value or saturation are at intermediate values. We consider two possible explanations of how high values of density can coincide with middling values of value and saturation: characteristics of the spectral reflectance curves, or the complexity of the neural signals that underlie the emergent property.     

Multi-key FHE without ciphertext-expansion in two-server model

López-Alt et al.(STOC12)put forward a primitive called multi-key fully homomorphic encryption(MKFHE),in which each involved party encrypts their own data using keys that are independently and randomly chosen whereby arbitrary computations can be performed on these encrypted data by a final collector.Subsequently,several superior schemes based on the standard assumption(LWE)were proposed.Most of these schemes were constructed by expanding a fresh GSW-ciphertext or BGV-ciphertext under a single key to a new same-type ciphertext of the same message under a combination of associated parties’keys.Therefore,the new ciphertext’s size grew more or less linearly with an increase in the number of parties.In this paper,we proposed a novel and simple scheme of MKFHE based on LWE without increasing the size of the ciphertext in the two non-collusion server model.In other words,each party first independently shares their own data between two servers and each server only needs a one-round communication with another to construct a ciphertext of the same plaintext under a sum of associated parties’keys.Our new ciphertext under multiple keys has the same size as that of the original one with only one-round communication between two servers.The communication complexity is O(kmlogq)-bit,where k is the number of input ciphertexts involved,m is the size of a GSW-ciphertext and q is a modulus.In conclusion,we proved that our scheme is CPA-secure against semi-honest adversaries.     

烟囱圆形板式基础计算分析

玻璃厂窑炉烟囱因其受风荷载、地震荷载以及筒身的附加弯矩等作用,其基础设计尤为重要,文章讨论了钢筋混凝土烟囱应用最为广泛的圆形板式基础的计算方法。     

焦炉气甲烷化过程模拟计算顺序的确定

序贯模块法广泛应用于化工过程流程模拟。对于具有回路的化工过程,其计算顺序的确定是很重要的问题。本文针对有回路的焦炉气甲烷化流程进行了分析,确定了其模拟计算时的计算顺序,对焦炉气甲烷化流程的研究具有很好的参考价值。     

某屋架下弦屈曲的检测分析

某在建厂房因柱基位移导致屋架下弦屈曲,通过现场检测和结构计算,分析结构变形的原因,提出结构处理方案.     

缓圆曲线上任意点的测量坐标计算

根据坐标的平移、旋转原理和正算、反算公式，推出缓圆曲线上任意点测量坐标的计算公式，以便在道路施工中采用坐标制放线。