分布式多元随机动态场景生成及快速精准场景降维算法

高比例新能源及多源耦合是电力系统发展的重要特征,这也为系统稳定经济运行提出了新挑战。该文以园区型多能系统为对象,研究了分布式多元随机动态场景分析,从多时空角度有效量化不确定因素给系统造成的影响,可为系统灵活重构、多维度协同运行与决策提供有力模型与场景支撑。首先由预测误差驱动拟合多元功率预测误差概率分布,全面反映随机功率出力信息,提高模型泛化性;以时序相关范围参数为数据驱动关联变量,高效动态控制波动强度;最终场景生成利用逆变换映射思想保证置信度。然后针对典型场景提取,提出一种综合递归聚类思想的多段嵌套削减算法,结合改进Wasserstein距离指标,兼具准确、时效、稳定方面的优势。最后由对比实验论证该方法的前沿有效性。     

基于改进蚁群算法的三维路径规划

针对移动机器人在海水环境中的三维路径规划问题容易陷入局部最优和收敛慢等瑕疵，根据三维环境全局信息来改进蚁群算法以提高实时性和收敛速度。改进蚁群算法的启发函数，采用局部信息和全局信息结合动态地改进信息素更新方式，以及根据三维空间中路径的平坦程度和光滑度二阶微分分别增加了一阶微分和二阶微分来再次修改信息素更新规则。仿真对比实验结果显示改进后的蚁群算法克服了收敛速度慢、容易陷入局部最优的缺点。     

A High-Throughput Screening System Based on Fluorescence-Activated Cell Sorting for the Directed Evolution of Chitinase A

Chitinases catalyze the degradation of chitin, a polymer of N-acetylglucosamine found in crustacean shells, insect cuticles, and fungal cell walls. There is great interest in the development of improved chitinases to address the environmental burden of chitin waste from the food processing industry as well as the potential medical, agricultural, and industrial uses of partially deacetylated chitin (chitosan) and its products (chito-oligosaccharides). The depolymerization of chitin can be achieved using chemical and physical treatments, but an enzymatic process would be more environmentally friendly and more sustainable. However, chitinases are slow-acting enzymes, limiting their biotechnological exploitation, although this can be overcome by molecular evolution approaches to enhance the features required for specific applications. The two main goals of this study were the development of a high-throughput screening system for chitinase activity (which could be extrapolated to other hydrolytic enzymes), and the deployment of this new method to select improved chitinase variants. We therefore cloned and expressed the Bacillus licheniformis DSM8785 chitinase A (chiA) gene in Escherichia coli BL21 (DE3) cells and generated a mutant library by error-prone PCR. We then developed a screening method based on fluorescence-activated cell sorting (FACS) using the model substrate 4-methylumbelliferyl β-d-N,N′,N″-triacetyl chitotrioside to identify improved enzymes. We prevented cross-talk between emulsion compartments caused by the hydrophobicity of 4-methylumbelliferone, the fluorescent product of the enzymatic reaction, by incorporating cyclodextrins into the aqueous phases. We also addressed the toxicity of long-term chiA expression in E. coli by limiting the reaction time. We identified 12 mutants containing 2–8 mutations per gene resulting in up to twofold higher activity than wild-type ChiA.     

Effect of paddy variety,milling status and aeration on the progeny emergence of Sitophilus oryzae L. (Coleoptera: Curculionidae)

In Sri Lanka, insects cause enormous losses in stored paddy; the rice weevil Sitophilus oryzae is a devastating insect. The magnitude of losses in stored paddy caused by insects varies with characteristics of paddy but has not yet been fully investigated. Due to the increased concerns on the use of synthetic chemicals, safer alternative pest management strategies for stored paddy are needed. Objectives of this study were to determine the effect of paddy variety, milling status and nature of aeration on the progeny emergence of S. oryzae. The experimental design was a three-factor factorial, completely randomized design (CRD).Freshly emerged S. oryzae adults were introduced to un-milled paddy or milled/polished rice belonging to traditional and improved varieties. The samples were maintained either open or airtight, and the progeny adults emerged was determined at monthly intervals. Progeny emergence was lower in the traditional varieties, un-milled paddy and under air-tight condition compared to improved varieties, milled/polished rice and aerated samples, respectively. Overall, the improved variety Bg 300 exhibited the maximum resistance to infestation by S. oryzae. Furthermore, white-colour and long-grain varieties produced lower progeny of S. oryzae than red-colour and short-grain varieties, respectively. It is concluded that the maximum protection in paddy/rice from insect infestation during storage can be achieved by using traditional varieties, stored as un-milled paddy (without dehusked) under airtight condition.     

山桐子产业在我国的开发利用情况

山桐子作为优良的木本油料树种,近年来受到越来越多的关注。论述了山桐子产业在我国的开发利用情况,主要包括山桐子国家扶持政策、良种选育、人工栽培、山桐子油食用安全性、山桐子油加工工艺及深加工应用情况,以期为山桐子产业的发展提供参考。     

综合改进奇异谱分解和奇异值分解的齿轮故障特征提取方法

针对齿轮故障特征微弱，在强背景噪声下难以有效提取的问题，提出了一种改进奇异谱分解（ISSD）结合奇异值分解（SVD）的齿轮故障特征提取方法。针对奇异谱分解（SSD）算法中模态参数需凭经验选取的缺陷，基于散布熵优化算法对SSD算法进行了改进，在得到既定的一组奇异谱分量的基础上，根据峭度值最大准则筛选出了最佳奇异谱分量并进行了SVD处理，采用奇异值能量标准谱自适应地确定了信号重构阶数以还原信号和提高降噪效果。最后对信号进行包络解调以提取齿轮故障特征，将所提方法运用到仿真信号和齿轮实测信号中，并同传统包络谱、SSD包络谱以及经验模态分解结合SVD(EMD-SVD)方法进行了对比分析，结果表明，所提方法的降噪和特征提取效果更佳，能够更加有效地实现齿轮故障的判别。     

透明红外硫系薄膜非均匀性检测及影响因素

薄膜非均匀性的无损检测对于制备大面积高质量的红外透明薄膜尤为重要。针对红外薄膜光学均匀性难以获取的困难,提出了一种同时获得单层透明红外薄膜厚度和折射率均匀性的无损检测方法。实验上,通过磁控溅射法在二氧化硅衬底上制备了厚度约1.4μm红外透明Ge-Sb-Se硫系薄膜,然后在该薄膜上标定出36个80μm×80μm区域,利用显微傅里叶红外光谱仪测得该36个区域的透射谱,通过分段滤波的方法滤除背景噪声,运用改进的Swanepoel方法计算得到了薄膜每一个区域的厚度和折射率,进而精确获得该薄膜的厚度和折射率均匀性,结果表明精度优于0.5%。     

复杂地形下风力机尾流数值模拟研究和激光雷达实验对比

以CFD数值计算和实验相结合的方法,对处于中国西南某多山地区陆上风电场的尾流特性进行研究,验证不同数值方法在复杂地形的适用性。首先采用2台激光雷达,测量目标风力机一个月内的自由来流风速和尾流廓线,在地形上坡加速效应下,不同大气稳定度下目标风力机的自由来流风速廓线均呈负梯度。然后分别采用经典致动盘和改进致动盘法,模拟目标风力机在主风向下的尾流发展。不同于只有风速与压降关系的经典致动盘法,改进致动盘法更考虑了叶片几何和气动参数(尺寸信息、攻角、桨距角、升阻力系数等)。通过与后置激光雷达尾流测试结果对比,这2种基于CFD技术的数值模拟方法,计算网格相同,计算时间相当,且均能较好地模拟因为复杂地形而引起的尾流偏转;其中改进致动盘的尾流形状与激光雷达相似,速度亏损也更接近激光雷达结果。因此,改进致动盘法更适合于复杂地形条件下风场模拟,较好平衡了计算的效率与精度。     

采用磁流变阻尼器的人体座椅悬架系统模糊ANFIS-PID复合控制技术研究

对座椅悬架用单出杆式磁流变阻尼器进行阻尼特性试验,并借助MATLAB多项式拟合工具箱对改进多项式模型中未知参数进行辨识。分析人体振动特性,建立五自由度人体座椅悬架模型。综合模糊控制器与自适应模糊神经推理系统(Adaptive Neuro-Fuzzy Inference System,ANFIS)整定PID控制器的优点,提出一种模糊ANFIS-PID复合控制策略。采用正弦信号作为外界激励,分别对被动悬架、传统模糊控制、ANFIS-PID控制及模糊ANFIS-PID复合控制人体座椅悬架系统进行仿真分析。结果表明,辨识得出的参数和建立的改进多项式模型均可满足后续仿真要求;采用模糊ANFIS-PID复合控制策略的隔振效果明显优于传统模糊控制及ANFIS-PID控制,能有效改善人体座椅悬架系统的行驶平顺性及驾乘人员乘坐舒适性。     

改良森吉米尔法退火炉中带钢的氧化研究

总结了带钢经过改良森吉米尔退火炉后的4种氧化状态,通过现场实际生产经验的总结和数据积累,分析了带钢产生表面氧化的关键因素,指出了实际操作中应该注意的事项,为以后的设计调试及生产提供了参考。