分布式多元随机动态场景生成及快速精准场景降维算法

高比例新能源及多源耦合是电力系统发展的重要特征,这也为系统稳定经济运行提出了新挑战。该文以园区型多能系统为对象,研究了分布式多元随机动态场景分析,从多时空角度有效量化不确定因素给系统造成的影响,可为系统灵活重构、多维度协同运行与决策提供有力模型与场景支撑。首先由预测误差驱动拟合多元功率预测误差概率分布,全面反映随机功率出力信息,提高模型泛化性;以时序相关范围参数为数据驱动关联变量,高效动态控制波动强度;最终场景生成利用逆变换映射思想保证置信度。然后针对典型场景提取,提出一种综合递归聚类思想的多段嵌套削减算法,结合改进Wasserstein距离指标,兼具准确、时效、稳定方面的优势。最后由对比实验论证该方法的前沿有效性。     

基于功效系数法和改进层次分析法的采空区稳定性评价研究

研究采空区稳定性评价新方法或模型对于提高采空区稳定性状况评判的可靠度、保证地下矿山的生产安全具有重要意义。选取工程地质环境、岩体质量、采空区几何参数和围岩应力四方面的因素作为采空区稳定性的主要影响因子，构建了地下矿山采空区稳定性评价指标体系。基于功效系数法原理，采用三标度改进层次分析法确定各评价指标的权重系数，建立了采空区稳定性评判分级新模型。将所建立模型运用到盘龙铅锌矿采空区稳定性评价研究，评判结果与工程实际相吻合，验证了基于功效系数法的采空区稳定性评判分级模型的适用性和可靠性，为采空区稳定性评价提供了一种新方法。     

基于改进蚁群算法的三维路径规划

针对移动机器人在海水环境中的三维路径规划问题容易陷入局部最优和收敛慢等瑕疵，根据三维环境全局信息来改进蚁群算法以提高实时性和收敛速度。改进蚁群算法的启发函数，采用局部信息和全局信息结合动态地改进信息素更新方式，以及根据三维空间中路径的平坦程度和光滑度二阶微分分别增加了一阶微分和二阶微分来再次修改信息素更新规则。仿真对比实验结果显示改进后的蚁群算法克服了收敛速度慢、容易陷入局部最优的缺点。     

面向混批加工的装夹方案选择与线平衡集成优化方法

针对一类多种箱体类同族零件混批加工的装夹方案选择与线平衡问题，提出一种集成优化方法。考虑操作优先关系和生产线产能约束，以节拍、夹具种类、平衡率和整线平滑系数为优化目标，分析了不同装夹组合下的各零件生产指标。引入多零件批量比重系数，并采用改进遗传算法对模型进行求解。最后，以某企业两缸体混批生产线为例，验证了该方法的有效性和高效性。     

基于混合模型与改进多分类马田系统的控制图模式识别

为提高生产过程中产品质量的智能监控水平，提出基于时间序列混合模型及改进多分类马田系统的控制图模式识别算法。选用时间序列混合模型对控制图实时数据进行特征提取；改进马田系统的阈值计算方法并制定多类判别准则，将表征的特征向量代入改进多分类马田系统分类器中进行特征约减及模式识别。最后，将该识别算法应用于控制图公开数据集及生产案例中，以验证算法的有效性，并与其他算法对比了分析，结果表明，基于时间序列混合模型及改进多分类马田系统算法能简化识别系统，识别精度高，是一种更为有效的控制图模式识别方法。     

改良十六烷基三甲基溴化铵-磁珠核酸提取方法及在植物转基因检测中的应用

目的 建立一种快速、高效、便捷的植物基因组DNA提取方法并运用于植物转基因检测。方法 改良传统的十六烷基三甲基溴化铵(cetyltrimethylammonium ammonium bromide, CTAB)方法, 并结合磁珠吸附基因组DNA, 配合核酸自动提取系统提取水稻和加工米粉中的核酸, 荧光聚合酶链式反应(polymerase chain reaction, PCR)检测Bt63大米转基因成分, 并与另外4种提取方法的结果进行对比, 评价提取效果。结果 5种不同方法中CTAB-磁珠法提取的样品基因组DNA浓度和质量最佳, 荧光PCR检测低浓度Bt63转基因成分(0.01%)的Ct值最小, 检测结果最好。结论 该方法可高效快速地提取植物核酸, 在低含量的转基因成分检测中相比其他几种方法具有绝对优势。     

A High-Throughput Screening System Based on Fluorescence-Activated Cell Sorting for the Directed Evolution of Chitinase A

Chitinases catalyze the degradation of chitin, a polymer of N-acetylglucosamine found in crustacean shells, insect cuticles, and fungal cell walls. There is great interest in the development of improved chitinases to address the environmental burden of chitin waste from the food processing industry as well as the potential medical, agricultural, and industrial uses of partially deacetylated chitin (chitosan) and its products (chito-oligosaccharides). The depolymerization of chitin can be achieved using chemical and physical treatments, but an enzymatic process would be more environmentally friendly and more sustainable. However, chitinases are slow-acting enzymes, limiting their biotechnological exploitation, although this can be overcome by molecular evolution approaches to enhance the features required for specific applications. The two main goals of this study were the development of a high-throughput screening system for chitinase activity (which could be extrapolated to other hydrolytic enzymes), and the deployment of this new method to select improved chitinase variants. We therefore cloned and expressed the Bacillus licheniformis DSM8785 chitinase A (chiA) gene in Escherichia coli BL21 (DE3) cells and generated a mutant library by error-prone PCR. We then developed a screening method based on fluorescence-activated cell sorting (FACS) using the model substrate 4-methylumbelliferyl β-d-N,N′,N″-triacetyl chitotrioside to identify improved enzymes. We prevented cross-talk between emulsion compartments caused by the hydrophobicity of 4-methylumbelliferone, the fluorescent product of the enzymatic reaction, by incorporating cyclodextrins into the aqueous phases. We also addressed the toxicity of long-term chiA expression in E. coli by limiting the reaction time. We identified 12 mutants containing 2–8 mutations per gene resulting in up to twofold higher activity than wild-type ChiA.     

Effect of paddy variety,milling status and aeration on the progeny emergence of Sitophilus oryzae L. (Coleoptera: Curculionidae)

In Sri Lanka, insects cause enormous losses in stored paddy; the rice weevil Sitophilus oryzae is a devastating insect. The magnitude of losses in stored paddy caused by insects varies with characteristics of paddy but has not yet been fully investigated. Due to the increased concerns on the use of synthetic chemicals, safer alternative pest management strategies for stored paddy are needed. Objectives of this study were to determine the effect of paddy variety, milling status and nature of aeration on the progeny emergence of S. oryzae. The experimental design was a three-factor factorial, completely randomized design (CRD).Freshly emerged S. oryzae adults were introduced to un-milled paddy or milled/polished rice belonging to traditional and improved varieties. The samples were maintained either open or airtight, and the progeny adults emerged was determined at monthly intervals. Progeny emergence was lower in the traditional varieties, un-milled paddy and under air-tight condition compared to improved varieties, milled/polished rice and aerated samples, respectively. Overall, the improved variety Bg 300 exhibited the maximum resistance to infestation by S. oryzae. Furthermore, white-colour and long-grain varieties produced lower progeny of S. oryzae than red-colour and short-grain varieties, respectively. It is concluded that the maximum protection in paddy/rice from insect infestation during storage can be achieved by using traditional varieties, stored as un-milled paddy (without dehusked) under airtight condition.     

山桐子产业在我国的开发利用情况

山桐子作为优良的木本油料树种,近年来受到越来越多的关注。论述了山桐子产业在我国的开发利用情况,主要包括山桐子国家扶持政策、良种选育、人工栽培、山桐子油食用安全性、山桐子油加工工艺及深加工应用情况,以期为山桐子产业的发展提供参考。     

综合改进奇异谱分解和奇异值分解的齿轮故障特征提取方法

针对齿轮故障特征微弱，在强背景噪声下难以有效提取的问题，提出了一种改进奇异谱分解（ISSD）结合奇异值分解（SVD）的齿轮故障特征提取方法。针对奇异谱分解（SSD）算法中模态参数需凭经验选取的缺陷，基于散布熵优化算法对SSD算法进行了改进，在得到既定的一组奇异谱分量的基础上，根据峭度值最大准则筛选出了最佳奇异谱分量并进行了SVD处理，采用奇异值能量标准谱自适应地确定了信号重构阶数以还原信号和提高降噪效果。最后对信号进行包络解调以提取齿轮故障特征，将所提方法运用到仿真信号和齿轮实测信号中，并同传统包络谱、SSD包络谱以及经验模态分解结合SVD(EMD-SVD)方法进行了对比分析，结果表明，所提方法的降噪和特征提取效果更佳，能够更加有效地实现齿轮故障的判别。