超高效液相色谱-串联质谱法测定白酒中9种甜味剂

目的建立准确、快速检测白酒中安赛蜜、糖精钠、甜蜜素、阿斯巴甜、三氯蔗糖、纽甜、阿力甜、甜菊糖苷和甜菊双糖苷9种甜味剂的超高效液相色谱-串联质谱分析方法。方法样品经沸水浴加热除去乙醇,采用超高效液相色谱-串联质谱法进行检测。以水(含10 mmol/L乙酸铵)和甲醇为流动相, HSS T3色谱柱(2.1 mm×100 mm,1.7μm)进行分离,电喷雾离子化多反应监测模式检测。结果 9种甜味剂在相应线性范围内关系良好,相关系数均≥0.999;方法回收率为85.2%～102.8%,相对标准偏差(RSD)为3.8%～7.3%;检出限为0.3～1.5μg/kg。结论该方法操作简单快速、重现性好,可用于白酒中9种甜味剂的检测。     

2018年湖北省食品中苯甲酸、山梨酸、糖精钠的抽检结果分析

目的 了解湖北省食品中苯甲酸、山梨酸、糖精钠的使用安全状况, 为省食品安全监管工作提供科学依据。方法 对2018年各类食品按照相关标准进行检验和评价, 对检测结果进行分析。结果 2018年共检测食品887批次, 总合格率为94.25%。苯甲酸、山梨酸超标使用的情况分布在水果制品、豆制品和餐饮食品中。结论 2018年湖北省食品安全状况总体良好, 但也存在一些问题。相关部门应加大对小作坊的监督力度, 规范生产, 保障消费者的身体健康。     

茶饮料中糖精钠的测定

建立了茶饮料中糖精钠的荧光分光光度测定方法。样品前处理采用盐酸酸化,加入氯化钠,乙醚萃取后再用2 g/L碳酸钠溶液洗涤乙醚萃取层,加热除去乙醚定容后用荧光光度计测定。测试条件为：激发波长265 nm,发射波长475 nm。方法检测限为2 mg/kg,线性范围在2～60 mg/kg之间,变异系数为1.97%,回收率96.8%。     

HPLC同时测定豆制品中常见的3种食品添加剂

采用反相高效液相色谱法快速、准确测定豆制品中的苯甲酸、山梨酸和糖精钠。3种添加剂在Zorbax sb-C18色谱柱上分离,甲醇-乙酸铵溶液(5∶95,V∶V)为流动相洗脱,流速1.0mL/min,15min内实现良好分离。方法检出限为0.5mg/kg,变异系数为0.67%～1.23%,回收率在97.7%～99.7%。该方法简便准确,适合豆制品的日常检测。     

液相色谱法测定白酒中安赛蜜和糖精钠含量

建立了高效液相色谱测定白酒中安赛蜜、糖精钠的方法。采用在沸水浴条件下,将白酒样品50.0 g用旋转蒸发仪减压蒸发至近干后用水定容至10 mL,进液相色谱分析;用AQ-C18色谱柱（250×4.6 mm）,以0.02 mol/L的乙酸铵-甲醇（90∶10）为流动相,二极管阵列检测器在波长220 nm进行检测。结果表明,该方法的检出限低至安赛蜜0.014 mg/kg,糖精钠0.019 mg/kg,被测样品加标回收率：安赛蜜为97.5%,糖精钠102.0%。     

Cocaine-induced suppression of saccharin intake: A model of drug-induced devaluation of natural rewards.

In Experiment 1, water-deprived Sprague-Dawley rats were given 5 min access to saccharin. This tube retracted, an empty tube advanced, and the rats were given 1 hr to lick the empty tube on a fixed-ratio 10 lick contingency to self-administer saline or cocaine (0.33 mg/infusion) via an intravenous catheter. The results showed that rats avoided intake of saccharin after saccharin-cocaine pairings and that greater avoidance of the gustatory cue was associated with greater cocaine self-administration. In Experiment 2, a similar dose-response function was obtained with either the empty tube or a lever as the operant. In Experiment 3, avoidance of the saccharin cue and the propensity to self-administer cocaine were maintained after at least 1 month of abstinence. As such, this paradigm may be useful as a model of cue-induced craving and drug-induced devaluation of natural rewards. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Estimated intake of intense sweeteners from non-alcoholic beverages in Denmark

In 1999, 116 samples of non-alcoholic beverages were analysed for the intense sweeteners cyclamate, acesulfame-K, aspartame and saccharin. High contents of cyclamate close to the maximum permitted level in 1999 of 400 mg l^-1 were found in many soft drinks. The estimated intake of the sweeteners was calculated using the Danish Dietary Survey based on 3098 persons aged 1-80 years. The estimated intake with 90th percentiles of 0.7, 4.0 and 0.2 mg kg^-1 body weight (bw) day^-1 for acesulfame-K, aspartame and saccharin, respectively, was much lower than the acceptable daily intake (ADI) values of 15, 40 and 2.5 mg kg^-1 bw day^-1 for acesulfame-K, aspartame and saccharin, respectively. However, the 90th percentile of the estimated cyclamate intake in 1-3 year olds was close to the ADI value of 7 mg kg^-1 bw day^-1; and the 99th percentile in the 1-10 year olds far exceeded the ADI value. Boys aged 7-10 years had a significantly higher estimated intake of cyclamate than girls. The 90th percentile for the whole population was 1.8 mg kg^-1 bw day^-1. After the reduction in the maximum permitted level in the European Union in 2004 from 400 to 250 mg cyclamate l^-1, the exposure in Denmark can also be expected to be reduced. A new investigation in 2007 should demonstrate whether the problem with high cyclamate intake is now solved.     

毛细管区带电泳法测定食品中山梨酸、苯甲酸和糖精的含量

采用毛细管区带电泳(CZE),以10mmol/L硼砂和0.1%草酸(pH8.26)为缓冲液,分离电压20kV,检测波长230nm,压差进样时间10s,以焦性没食子酸为内标,在7min内分离和测定了食品中山梨酸、苯甲酸和糖精的含量。线性范围:山梨酸4～640mg/L、苯甲酸4～640mg/L、糖精4～1000mg/L;样品加标回收率在95.0%～98.4%,相对标准偏差(RSD)均小于4%。方法简便、快速、准确。     

葡萄酒中糖精钠、甜蜜素和柠檬酸的测定

介绍了用高效液相色谱法测定葡萄酒中糖精钠、甜蜜素和柠檬酸的原理和具体方法.     

离子迁移谱法测定饮料中的糖精钠和痕量马来酸

目的利用电喷雾-高效离子迁移谱(ESI-HPIMS)对饮料中常见的甜味剂糖精钠和有可能残存的痕量马来酸进行检测。方法通过在饮料中加入糖精钠和马来酸标准品来模拟添加剂存留情况,添加后的饮料经简单的稀释,过滤等前处理,直接进样即可测定。结果研究结果表明,仪器对糖精钠和马来酸标准品的灵敏度达到了10~(-10)数量级,最低检出限为0.1 ng/μL,6组平行数据的相对标准偏差(RSD)在1.2%~7.2%,线性相关系数均达到了r~2大于0.99,单次测量时间低于10 s。结论实现了糖精钠和痕量马来酸的快速分析检测。