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1.
Over the past decade, telomeres have attracted increasing attention due to the role they play in human fertility. However, conflicting results have been reported on the possible association between sperm telomere length (STL) and leukocyte telomere length (LTL) and the quality of the sperm parameters. The aim of this study was to run a comprehensive study to investigate the role of STL and LTL in male spermatogenesis and infertility. Moreover, the association between the sperm parameters and 11 candidate single nucleotide polymorphisms (SNPs), identified in the literature for their association with telomere length (TL), was investigated. We observed no associations between sperm parameters and STL nor LTL. For the individual SNPs, we observed five statistically significant associations with sperm parameters: considering a p < 0.05. Namely, ACYP2˗rs11125529 and decreased sperm motility (p = 0.03); PXK˗rs6772228 with a lower sperm count (p = 0.02); NAF1˗rs7675998 with increased probability of having abnormal acrosomes (p = 0.03) and abnormal flagellum (p = 0.04); ZNF208˗rs8105767 and reduction of sperms with normal heads (p = 0.009). This study suggests a moderate involvement of telomere length in male fertility; however, in our analyses four SNPs were weakly associated with sperm variables, suggesting the SNPs to be pleiotropic and involved in other regulatory mechanisms independent of telomere homeostasis, but involved in the spermatogenic process.  相似文献   
2.
现有的双目同步定位与建图(SLAM)都使用标准立体相机,所处环境为静态的假设会影响其在动态环境中的精度。 提 出了一种多焦距动态立体视觉 SLAM 方法,它克服了标准立体相机无法兼顾远距离和宽视场感知场景的缺点,并去除了动态物 体对 SLAM 的影响。 具体来说,对传统的立体校正方法进行了改进,并使用校正参数修正了特征点的位置,而不是整张图像,还 提出了一种自适应特征提取和匹配方法以增加多焦距图像的特征匹配数量。 综合使用多视图几何、区域特征流和相对距离检 测动态对象,剔除动态对象上的特征点。 在公开数据集 KITTI 上,该方法相对 ORB-SLAM3 和 DynaSLAM 的定位精度都提高了 6. 97% ,在自建数据集中,该方法的定位精度比 ORB-SLAM3 提高了 26. 64% ,比 DynaSLAM 提高了 32. 09% 。  相似文献   
3.
王振华  蒋军成  尤飞  李刚  庄陈浩  赵耀鹏  倪磊  潘勇  李丹 《化工学报》2021,72(10):5412-5423
近年氢能已迅速成为能源领域“新宠”,正在迎来快速发展的战略机遇期,但氢安全问题仍然是制约其发展的关键,尤以高压氢气储运设施泄漏后引发喷射火灾害较为突出。为了探究高压氢气泄漏过程并对其引发喷射火灾特性参数变化进行评估,本文采用理论分析和实例验证相结合的方法对两起高压氢气泄漏实验案例(90 MPa氢气瓶和6 MPa氢气管道)进行了研究。结果表明:通过模型精度检验,Abel-Nobel气体状态方程适用于当前常用的多种高压氢气储运设施泄漏过程的描述。基于Abel-Nobel气体状态方程、火焰尺寸模型、辐射分数模型和热辐射模型构建的高压氢气泄漏喷射火过程预测模型对实验案例中的泄漏出口气体质量流量、氢喷射火焰长度和辐射热场等的模拟计算结果与实验测量数据基本一致,验证了模型有效性及所含假设合理性。另外在计算中还需要结合实际情况充分考虑高压氢气储运设施发生泄漏时产生的能量损失以及等温流动过程,从而对模型预测精度进行修正。上述结论对于工程实际、氢能安全利用以及灾害预防等具有重要现实意义。  相似文献   
4.
Spinal muscular atrophy (SMA) is an autosomal recessive hereditary neuromuscular disease. Exon 7 and 8 of survival of motor neuron 1 (SMN1) gene or only exon 7 homology deletion leads to the failure to produce a full-length SMN gene. The copy number of SMN2 gene with high homology of SMN1 affects the degree of disease and was the target gene for targeting therapy, in which splicing silencer in intron 7 was the key to suppress the inclusion of exon 7. In this study, we projected to use CRISPR/Case 9 for the targeted editing of intronic-splicing silencer (ISS) sequence to promote the inclusion of SMN2 exon 7 and increase the production of SMN2 full-length (FL) gene expression. It happens that there was a protospacer adjacent motif (PAM) at one end of the ISS sequence according to the design of sgRNA. The recombinant vector of sgRNA HSMN2 CRISPR/Case 9 was constructed and transfected into HEK293 cells. Sequencing results showed that the ISS sequence could be edited accurately and targeting in the predicted direction, in which deleting small fragments, inserting small amounts and mutation. Quantitative analysis of RT-PCR products by restriction enzyme of DdeI digestion showed that the FL of SMN2 increased by 8% (P < 0.05). In the primary cultured chondrocytes of SMA mice, in which sgRNA HSMN2 CRISPR/Case9 recombinant vector transfection could increase the SMN2 FL gene by 23% (P < 0.05) and significantly improve SMN protein levels (P < 0.05). CRISPR/Case 9 is an effective tool for gene editing and therapy of hereditary diseases, but it is rarely reported in the treatment of SMA diseases. This study shows that CRISPR/Case 9 was first used for the precision target of ISS sequence editing, which can effectively promote the production of SMN2 FL gene expressions, in which there was an important clinical reference value.  相似文献   
5.
随着城市中汽车数量的大增,汽车与汽车之间的通信系统显得尤为重要。因此互联网在行车过程中起到了重要的作用,其中的车载网便扮演着物物通信的功能。本文提出行车过程中复杂特性的分发算法,首先对多个城市中的汽车进行汽车行动路线的统计与分析,其中包含了通信车辆之间的距离、所连车辆的数量分布以及聚集指标,从结论分析得到车与车之间联系的数量呈现一定的指数分布;并且在小范围内,聚集指标比较高。根据上述特性分,推算出一种车辆行进轨迹数据的分发算法,以便于进行车辆与车辆的实时通信,这样就能大大提高车辆行驶的有效性和降低车祸之间的发生。  相似文献   
6.
为明确低渗透储层构造裂缝长度定量表征方法,以四川盆地磨溪—高石梯地区寒武系龙王庙组为例,综合采用岩心裂缝统计及岩石力学实验方法,从构造应力场的角度推导低渗透储层构造裂缝长度的关系式。该方法将裂缝长度与裂缝体密度、应变能密度及岩体应力状态联系起来,建立了裂缝长度与裂缝体密度之间的定量计算关系。结果表明:裂缝长度与裂缝数量呈负指数幂关系;裂缝体密度与应变能密度呈正比线性关系;裂缝长度与裂缝体密度呈负指数幂关系。将推导的裂缝长度公式应用于磨溪—高石梯地区龙王庙组,数值模拟结果显示:裂缝体密度值普遍介于1~5 m2/m3,最高为9 m2/m3,高值区主要分布于断层及周边地区;裂缝长度主要介于1~20 m,断层及周边区域裂缝密而短,长度普遍小于3 m。   相似文献   
7.
《Journal of dairy science》2022,105(11):9286-9295
Holstein (HO) calves, 3-breed crossbred calves of Montbéliarde, Viking Red, and HO (MVH), and 3-breed crossbred calves of Normande, Jersey, and Viking Red (NJV) were compared for gestation length (GL), calf weight at birth (CW), calving difficulty (CD), and stillbirth (SB) in 2 research herds at the University of Minnesota. Calves were born from January 2009 to December 2019. For the St. Paul and Morris herds, HO calves (n = 1,121) were compared with MVH calves (n = 1,393) from primiparous and multiparous cows. For the single herd analysis at Morris, HO calves (n = 476), MVH calves (n = 922), and NJV calves (n = 405) were compared from primiparous and multiparous cows. Primiparous and multiparous births were analyzed separately because multiparous cows had multiple births, and CD and SB are likely different traits for primiparous and multiparous cows. Statistical analysis of GL, CW, CD, and SB included fixed effects of sex of calf, herd, breed group of calf, and year-season of calving. For the St. Paul and Morris herds, HO calves from primiparous (278 d) and multiparous (279 d) HO cows had shorter GL compared with MVH calves from primiparous (280 d) and multiparous (282 d) crossbred cows. The HO calves (39.4 and 43.2 kg, respectively) from primiparous and multiparous HO cows had lower CW compared with MVH calves (40.3 and 44.3 kg, respectively) from primiparous and multiparous crossbred cows. Calving difficulty and SB were not different for HO and MVH calves from primiparous and multiparous cows. For the single herd analysis at Morris, HO calves (278 and 279 d, respectively) from primiparous and multiparous HO cows had shorter GL compared with MVH calves (281 and 282 d, respectively) and NJV calves (282 and 282 d, respectively) from primiparous and multiparous crossbred cows. The CW of HO calves (38.6 and 42.0 kg, respectively) from primiparous and multiparous HO cows was lower compared with MVH calves (39.7 and 42.9 kg, respectively), but higher compared with NJV calves (35.1 and 38.0 kg, respectively) from primiparous and multiparous crossbred cows. Calving difficulty and SB did not differ for HO, MVH, and NJV calves from primiparous and multiparous cows. The longer GL for crossbred calves and higher CW for MVH calves did not increase CD and SB for primiparous and multiparous cows. Dairy producers may implement 3-breed rotational crossbreeding systems that include the HO, Jersey, Normande, Montbéliarde, and Viking Red breeds, and some breeds may increase GL and CW without an increase in CD and SB.  相似文献   
8.
9.
力密度法通常用于张拉索网结构等不需要考虑稳定性的结构体系的找形分析,但对于树状结构,由于其在竖向荷载作用下所有构件仅受到轴向压力的作用,除了找形以外还需要考虑构件稳定承载能力的问题。利用力密度法对树状结构进行找形分析的基础上,引入欧拉稳定承载力计算理论以考虑构件几何长度对稳定承载力的影响,并提出了相应的迭代算法。推导了适用于树状结构的修正迭代力密度理论计算式,通过改变每一个分枝的力密度进而达到优化分枝长度的目标。通过与已有计算结果的对比验证所提对于平面树状结构算法的可靠性,对空间树状结构进行分析验证所提算法对于复杂树状结构的适用性。算例分析表明:采用所提算法迭代100次即可得到收敛的结果,耗时5min左右;与数值逆吊法找形相比,避免了双单元法抗弯刚度不恰当而导致的计算不收敛问题。  相似文献   
10.
为了得到近距离下位煤层回采巷道合理步距,理论分析了己15煤层回采过程中采场支承压力与底板损伤特征;数值模拟了己15煤层底板应力时空演化规律、下位煤层巷道合理位置以及巷道应力与位移分布特征。研究得出,理论计算得到己15煤层回采后底板被动应力区域边界长度为106 m,揭示了己15-31040工作面、己15-31020工作面、己15-23160工作面依次开采时煤柱下方底板应力峰值由6338 MPa减小至5463 MPa、然后无明显变化的特征;结合底板被动应力区域边界长度、煤层与顶底板摩擦系数、煤柱压力影响角、煤炭资源回收率、安全掘进等因素,巷道采用外错上位煤柱25 m的布置方式,该布置方式下巷道处于低应力环境的特性,且可为巷道围岩控制提供一定的参考依据。  相似文献   
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