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Synthetic biology promises to simplify the construction of metabolic pathways by assembling the de- tached modules of the whole pathway. This gives new approaches for the microbial production of industrial products such as polyhydroxyalkanoates (PHA). In this study, to produce poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) by Pseudomonas stutzeri 1317 from unrelated carbon sources such as glucose, the phaCl-phaZ-phaC2 operon of P. stutzeri 1317 was knocked out to generate the PHA deficient mutant P. stutzeri 1317LF. Then three modules containing phaCahAReBRe, phaCahBReGep and phaCAhPah were introduced into P. stutzeri 1317LF separately The shake flask results indicated that the precursor supply and PHA synthase activity were the vital factors for the PHBHHx accumulation of P. stutzeri 1317LF. Furthermore, the PHBHHx accumulation of the recombinants from different carbon resources were performed. The highest PHBHHx content was 23.7% (by mass) with 58.6% (by mole) 3HB fraction. These results provide basis for further improving the PHBHHx accumulation of P. stutzeri from unrelated carbon sources. 相似文献
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Synthetic biology promises to simplify the construction of metabolic pathways by assembling the de-tached modules of the whole pathway. This gives new approaches for the microbial production of industr... 相似文献
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IPEX98虽已落幕,但却给人留下了深刻的印象。施乐公司、海德堡公司及曼·罗兰公司等参展商在展出众多新产品的同时还在现场举办了多场免费技术讲座,使得原订为期3天的Seybold技术交流会不得不取消。因为既然能免费在展览会现场学到很多新知识,谁还愿花3000美元去听技术讲座呢? 相似文献
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