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以深共熔溶剂-水混合物(LAEG40)作为提取溶剂,红景天苷提取率为主要指标,酪醇提取率为辅助指标,在前期单因素实验的基础上,利用响应面法考察了液料比、提取温度、提取时间三个主要因素对红景天中红景天苷和酪醇同步提取的影响。得到的最佳提取条件为: 液料比12.05:1(mL/g),提取温度60 ℃,提取时间35 min。在此条件下LAEG40对红景天苷的提取率可达到19.3552?0.6604 mg/g,酪醇提取率可达到1.7211?0.0585 mg/g,远高于传统溶剂水、乙醇对红景天苷和酪醇的提取率。为实现LAEG40提取红景天苷和酪醇的回收,进行了大孔树脂吸附分离的研究。经过一系列优化,获得的最佳吸附分离条件为:选用SP-825树脂装柱,每10g树脂上样30 mL LAEG40提取液,洗脱剂选用80 %乙醇,洗脱体积40 mL,洗脱流速1 mL/min,在此条件下红景天苷和酪醇的回收率分别可达60.47 %和85.07 %。 相似文献
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Minghui Fan Shiying Xu Shuqin Xia Xiaoming Zhang 《European Food Research and Technology》2008,227(1):167-174
The purpose of this work was to prepare salidroside nano-liposomes by the ethanol injection method. To obtain the higher encapsulating
efficiency of salidroside nano-liposomes, several factors including salidroside-loading capacity, cholesterol, Tween 80, ion
strength and lipid concentration were investigated. The higher encapsulating efficiency of salidroside, 45%, was obtained
with cholesterol to lipid mass ratio of 1:4, Tween 80 and lipid to the molar ratio of 1:2, and ion strength in a range 20–50 mmol/L.
With the optimization operation, the particles of nano-liposomes were below 100 nm and zeta potential was in the range of
−10 and −20 mV. The release study of salidroside in vitro from nano-liposomes exhibited a prolonged release profile as studied
over a period of 24 h. 相似文献
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红景天苷的碘标记及其在小鼠体内的分布 总被引:2,自引:0,他引:2
通过131碘标记红景天苷以探索红景天苷在神经母细胞(SH-SY5Y)中的摄取及在小鼠体内的代谢分布.采用氯胺-T法对红景天苷进行131碘标记;以聚酰胺薄膜为支持介质、V三氯甲烷:V甲醇:V丙酮:V水=6:3:1:1的下层液为展开剂,测定标记率及标记物放化纯;分析神经母细胞SH-SY5Y及肿瘤细胞MCF-7对131I-红景天苷的摄取;KM小鼠尾静脉注射131I-红景天苷(1.85 MBq/只,n=5),于5、10、30、60、120、240 min分别取心、肝、肺、肾、脾、肌、骨、脑、肠、血,称重、计数,计算每克组织百分注射剂量率(%ID/g).结果表明,131I-红景天苷标记率达98%,其放化纯在1、4、20 d分别为98.5%、97.3%、97.1%;SH-SY5Y对131I-红景天苷基本无摄取,在0.5-4 h内摄取维持在0.035%左右,而MCF-7则为0.1%;131I-红景天苷在体内主要通过肝代谢、肾排泄,其中肝和肾5 min%ID/g组织分别为7.71%和11.32%,4 h则分别下降为0.36%和0.3%;血液中清除也较快,5 min时为6.41%,4 h为0.35%;在脑中虽分布较少,但清除较慢,5 min时为0.27%,4 h为0.11%;在心、肺、脾、肌、骨及肠中分布不多.结论是,碘标红景天苷标记率高,标记物稳定;神经母细胞对131I-红景天苷基本无摄取. 相似文献
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Salidroside (8-O-β-D-glucoside of tyrosol), a plant-derived natural product, is used for treatment of hypoxia, fatigue and aging diseases. The availability of salidroside is restricted since it is extracted from 3-5 years old Rhodiola roots, which grow very slowly in the cold region of northern hemisphere of Earth. Our laboratory has constructed an engineered Escherichia coli and established a fermentation process to produce salidroside from glucose. In this article, nine macroporous resins from polarity to non-polarity, including NKA-9, S-8, AB-8, SP825, D101, LSA-8, LX-12, LX-18 and LX-68 resins, were tested to separate salidroside from fermentation broth. After static and dynamic experiments, the weakly polar SP825 resin had a better separation efficiency among nine resins. The adsorption kinetic and isotherm of salidroside on the SP825 resin were determined, and the pseudo-second-order kinetic model and Langmuir model could be fitted well. The effects of the pH on adsorption and ethanol concentration on desorption were investigated, and an optimal separation process was established. The adsorption for salidroside in the SP825 resin column was conducted with loading 150 ml at pH 7, and desorpted by washing 50 ml of 80% ethanol solution. Under the best process conditions, the purity and yield of salidroside in the final product were 91.6% and 74.0%, respectively. The results showed that the macroporous SP825 resin would be feasible and effective to prepare salidroside and has promising application in the downstream process of microbial fermentation. 相似文献
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红景天苷纳米脂质体的制备及若干工艺参数的优化 总被引:1,自引:0,他引:1
本实验首先比较了薄膜法、超声法、冻融法和反相蒸发法对红景天苷脂质体包封率的影响,结果表明红景天苷低浓度下,不同方法制得的脂质体包封率影响依次为冻融法>反相蒸发法>超声法>薄膜法,高浓度下的包封率影响不大。粒径测定表明粒子均处于纳米尺度。其次,对影响红景天苷纳米脂质体制备的反相蒸发法工艺参数进行了优化。结果表明,随着载量的降低包封率呈现上升后趋于平缓的变化。胆固醇/卵磷脂质量比1:2的嵌入量得到的脂质体包封率最高。吐温80对脂质的增溶具有十分明显的作用,2%质量分数可使得脂质体的包封率达到约80%。水合介质的离子强度对包封率的影响较大,脂质体在0.05mol/L、pH7.0的磷酸盐缓冲液体系中包封率较高。 相似文献