A multi-sample design for assessing the comedolytic activity of topical products

Background: Facial comedolytic studies allow testing of a maximum of two products. The upper back provides a larger area with a more uniform distribution of microcomedones for comedolytic studies. Objective: To design a multi‐sample method for assessing comedolytic activity of topical products on the back. The effect of season on product discrimination was also explored. Methods: Three cleansing formulations (products B, C and D), a negative water control and 0.025% Retin‐A^® cream (positive control) were tested. Seven subjects were recruited in summer and nine in fall. Products were applied for 8 weeks, comedolysis was assessed by visually evaluating cyanoacrylate follicular biopsies taken at baseline and post‐treatment. Results: In all data sets (summer, fall and combined), sites treated with Retin‐A^® had a significantly lower number of microcomedones as compared to the negative water control. In addition, cleansers B and D showed a significant reduction from baseline in the fall and combined (summer and fall) data, but not in summer data alone indicating different responses to treatment during the year. Conclusions: The design was sensitive enough to detect differences between cleansing formulations under normal washing conditions. Reduced sensitivity observed during summer suggests hot humid conditions may decrease the comedolytic performance of topical products.     

液质联用技术检测祛痘类化妆品中西咪替丁等21种非法添加药物

建立了祛痘类化妆品中西咪替丁、四环素类、喹诺酮类等21种禁用物质的液质联用检测方法.以饱和氯化钠分散样品,质量分数0.5％甲酸-乙腈提取,-20℃冷冻10 min,10000 r/min冷冻离心取上清液过滤后测定.以质量分数0.1％甲酸乙腈-0.1％甲酸水溶液作为流动相梯度洗脱,流速为0.4 mL/min.采用正离子模...     

祛痘化妆品用中药提取物的抗炎作用研究

为研究祛痘化妆品川中药组方提取物的抗炎活性,采用二甲苯致小鼠耳廊肿胀、1％角叉荣胶致小鼠后足跖肿胀方法．测定致炎小鼠两耳的质量差和后足炎症因子IL-1α、PGE2及蛋白含量,研究中药组办提取物的抗炎活性。结果发现．中药提取物高（200mg／kg）、中（100mg／kg）和低（50mg／kg）剂量组明显抑制了耳肿胀的发生;高剂量组在造模2h后能减轻角叉菜胶所致的足肿胀．与阴性对照组相比有显著性差异,其他组其他时间点未见明显区别;药物组动物IL-1a、PGE2的释放均旺著下降。试验表明祛痘化妆品用中药组方提取物具有显著的抗炎活性。     

广藿香精油抗炎祛痘功效研究

采用气相色谱-质谱联用技术对广藿香精油成分进行分析。通过细胞毒性实验确定广藿香精油对人急性单核细胞(THP-1)的安全质量浓度。利用痤疮丙酸杆菌(P.acnes)刺激THP-1建立的特异性痤疮炎症细胞模型考察广藿香精油对炎症因子IL-1β的影响。通过荧光实时定量逆转录聚合酶链反应(qRT-PCR)初步探讨广藿香精油抗炎祛痘的作用机制。研究结果表明:广藿香精油在最大安全质量浓度12.5 mg/L时,对炎症细胞模型中P.acnes活性影响较小,对IL-1β的分泌具有明显的抑制作用(抑制率66.54%),可显著降低细胞内Toll样受体2(TLR2)mRNA的转录(抑制率73.73%),从而起到抑制炎症信号转导和继发性炎症介质生成的作用。     

Activity of capryloyl collagenic acid against bacteria involved in acne

Capryloyl collagenic acid (Lipacide C8Co) has similar bacteriostatic activity in vitro to that of benzoyl peroxide towards the bacteria found in acne lesions ( Staphylococcus aureus, Staphylococcus epidermidis and Propionibacterium acnes ) (MIC between 1 and 4 mg ml⁻¹ for C8Co, and between 0.5 and 5 mg ml⁻¹ for benzoyl peroxide). The presence of Emulgine M8 did not affect the bacteriostatic activity of C8Co. A 4% w/v solution of C8Co (incorporating Emulgine M8) fulfilled the criteria for an antiseptic preparation as laid down by the French Pharmacopoeia (10th Edition), and had a spectrum 5 bactericidal activity according to the French Standard AFNOR NF T 72–151. The excellent cutaneous tolerance of capryloyl collagenic acid would indicate that an aqueous solution might be of value for topical treatment of the bacterial component of acne.     

口服痤疮治疗药物的应用现状

综述了口服痤疮治疗药物的临床应用现状,主要包括抗菌药、抑制毛囊皮脂腺导管角化异常药、抗雄性激素药、抗炎药和抗皮脂溢药、中草药等,为临床合理选用口服痤疮治疗药物及研发提供依据.     

祛痘功效添加剂的研究进展

简要介绍了痤疮的临床特征及发病机理,在此基础上,从去角质化、抗微生物及消炎、调节激素分泌等方面探讨了祛痘功效添加剂的筛选原则,并对植物源祛痘功效添加剂在抑制皮脂分泌、抑菌、消炎、祛疤痕方面的应用进行了述评,以期为祛痘活性成分的研究与开发提供参考．     

黄芩苷在功能性化妆品中的应用研究

简述了黄芩苷用于功能性化妆品的有效性。通过试验，制得了黄芩苷美白霜、黄芩苷抗粉刺嗜喱霜和黄芩苷防晒乳液。按有关标准要求对制得的产品进行了性能测试，并进行了试用效果观察和评价。试用结果为：黄芩苷美白霜总有效率为84．6％，黄芩苷抗粉刺嗜喱霜总有效率为85％。按Q8／T2410—1998测得黄芩苷防晒乳液的吸光度为1．65，具有高效防护紫外线照射效果，适合于户外工作使用。     

国内外祛痘化妆品管理现状的对比研究

分析国内外祛痘化妆品管理现状,提出我国祛痘化妆品监管建议.在概述祛痘化妆品功效、原料、作用原理的基础上,对比研究美国、欧盟、日本、韩国、加拿大以及我国祛痘化妆品在产品备案、功效原料、功效宣称等方面的管理现状,为我国祛痘化妆品后续监管提出建议.     

祛痘化妆品祛痘功效评价方法的研究

根据疗效化妆品的发展趋势和青春痘生成机理，提出用抑菌试验方法来评价祛痘化妆品的祛痘功效，同时应用这种方法来评价自制的祛痘化妆品功效，最后指出了该方法的优缺点。