响应面优化法在纳米乳液制备工艺研究中的应用

研究通过响应面优化法对纳米乳液制备工艺进行优化,考察了PEG-20氢化蓖麻油质量分数、平衡温度、搅拌速度对终产品综合评分的影响。研究发现,平衡温度和搅拌速度对综合评分有显著性影响。模型预测值与实测值间无显著性差异,模型预测性良好。     

50t/a路桥纳米致密化抗共振弹性防腐涂料工业化生产的研究

以三元聚合纳米乳液、乙烯-乙酸乙烯乳液为基料，以超细复合钛白粉、纳米CaCO3等为颜料，以及以超细弹性浆料、纳米抗老化剂、MNB-A型纳米致密化抗振剂为添加剂，严格控制相对分子质量，合理匹配流变控制剂等手段，开发出一系列高性能纳米致密化抗共振弹性防腐乳胶涂料。产品的涂膜、抗振性能和施工性能达到了国际先进水平，是符合环保要求的绿色路桥涂料。     

用阴离子Gemini 9 B-6-9 B乳化剂合成苯乙烯-丙烯酸丁酯共聚物纳米乳液

以壬基酚、1,6-二溴己烷、氯磺酸为主要原料合成的阴离子Gemini磺酸盐表面活性剂9 B-6-9 B为乳化剂,苯乙烯(St)、丙烯酸丁酯(BA)为单体,合成了乳胶粒径小于100 nm且分布均匀的苯乙烯-丙烯酸丁酯共聚物纳米乳液,考察了聚合温度、乳化剂用量、引发剂用量、单体配比、单体含量对乳液的影响,探讨了乳胶粒子成核机理。结果表明,随着温度的升高,乳胶粒子的平均粒径减小,转化率与凝胶率增大;随着乳化剂或引发剂用量的增加,乳胶粒子的平均粒径和凝胶率减小,转化率增大;随着软单体比例的增加,乳胶粒子的平均粒径与转化率增大,粒径分布变宽,凝胶率减小;单体用量增大,乳胶粒子的平均粒径与凝胶率增大,转化率降低;乳胶粒子的粒径呈单峰分布,可能是按胶束成核机理形成的。     

肉桂醛添加方式对乳清浓缩蛋白凝胶特性的影响

利用乳液和微胶囊的包载改善肉桂醛的亲水性,并将其添加至浓缩乳清蛋白溶液中,利用热诱导形成乳清浓缩蛋白凝胶,表征凝胶的流变特性、持水性、质构及微观结构,探究肉桂醛添加方式对所得凝胶特性的影响。结果表明,添加肉桂醛后,蛋白质凝胶的持水力显著增加。以乳液形式添加时,肉桂醛含量与凝胶的黏弹性、持水力、质构特性和网络结构的致密性呈正相关;以微胶囊形式添加时,肉桂醛可显著增强凝胶的黏弹性、持水力、质构特性和网络结构的致密性,但肉桂醛含量变化对凝胶特性的影响较小。对比两种添加方式,肉桂醛以微胶囊形式添加更能有效地调控蛋白凝胶的持水性和质构特性。     

食品纳米乳液的研究进展

纳米乳液载运体系能够改善活性成分的稳定性、溶解性和生物利用度,在食品领域受到广泛关注。文章主要对食品纳米乳液制备过程中芯材、油相和乳化剂的选择,及其对乳液体系理化性质的影响,以及制备方法、理化性质评价和应用前景等进行了综述。     

鱼油纳米乳的制备及贮藏稳定性研究

采用高压均质法,以乳清分离蛋白(WPI)乳化包埋鱼油制备鱼油纳米乳。采用单因素实验研究了主要因素对包封率和吸光比(SRI)的影响,并以包封率为指标,进行正交实验优化工艺配方。同时以不同压力处理的鱼油纳米乳为对照,分别考察了4、25℃条件下鱼油纳米乳的贮藏稳定性。结果表明:最佳工艺配方为乳清分离蛋白质量浓度25 g/L,鱼油添加量100 g/L,均质压力80 MPa,均质次数4次。采用最佳工艺配方制备的鱼油纳米乳的包封率为96.4%,SRI为0.113。稳定性实验结果表明鱼油经纳米乳包埋可显著提高鱼油氧化稳定性,高压处理有利于提高鱼油纳米乳的稳定性,鱼油纳米乳在4℃的贮藏稳定性高于25℃的贮藏稳定性,80 MPa处理鱼油纳米乳经4、25℃放置30 d乳析指数分别为0、1.4%,鱼油纳米乳经25℃放置60 d后过氧化值及渗漏率分别为4.25 meq/kg、5.89%。     

A Nano-Emulsion Platform Functionalized with a Fully Human scFv-Fc Antibody for Atheroma Targeting: Towards a Theranostic Approach to Atherosclerosis

Atherosclerosis is at the onset of the cardiovascular diseases that are among the leading causes of death worldwide. Currently, high-risk plaques, also called vulnerable atheromatous plaques, remain often undiagnosed until the occurrence of severe complications, such as stroke or myocardial infarction. Molecular imaging agents that target high-risk atheromatous lesions could greatly improve the diagnosis of atherosclerosis by identifying sites of high disease activity. Moreover, a “theranostic approach” that combines molecular imaging agents (for diagnosis) and therapeutic molecules would be of great value for the local management of atheromatous plaques. The aim of this study was to develop and characterize an innovative theranostic tool for atherosclerosis. We engineered oil-in-water nano-emulsions (NEs) loaded with superparamagnetic iron oxide (SPIO) nanoparticles for magnetic resonance imaging (MRI) purposes. Dynamic MRI showed that NE-SPIO nanoparticles decorated with a polyethylene glycol (PEG) layer reduced their liver uptake and extended their half-life. Next, the NE-SPIO-PEG formulation was functionalized with a fully human scFv-Fc antibody (P3) recognizing galectin 3, an atherosclerosis biomarker. The P3-functionalized formulation targeted atheromatous plaques, as demonstrated in an immunohistochemistry analyses of mouse aorta and human artery sections and in an Apoe^−/− mouse model of atherosclerosis. Moreover, the formulation was loaded with SPIO nanoparticles and/or alpha-tocopherol to be used as a theranostic tool for atherosclerosis imaging (SPIO) and for delivery of drugs that reduce oxidation (here, alpha-tocopherol) in atheromatous plaques. This study paves the way to non-invasive targeted imaging of atherosclerosis and synergistic therapeutic applications.     

藏蒲公英多糖纳米乳剂对小鼠外周血T淋巴细胞亚群CD4+、CD8+的影响

研究藏蒲公英多糖对小鼠免疫功能的调节作用.采用流式细胞术检测健康小鼠在灌胃0.1 ml质量分数0.85％生理盐水、0.1 ml质量分数1％藏蒲公英多糖水溶液和0.1 ml质量分数1％藏蒲公英多糖纳米乳剂情况下,外周血T淋巴细胞亚群CD4+、CD8+数量的动态变化.结果发现,藏蒲公英多糖和藏蒲公英多糖纳米乳剂均能够显著提高小鼠外周血T淋巴细胞亚群CD4+、CD8+水平,降低T淋巴细胞亚群CD4+、CD8+比值,尤以藏蒲公英多糖纳米乳剂效果最为显著.