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排序方式: 共有162条查询结果,搜索用时 15 毫秒
1.
目的 优化柱前衍生高效液相色谱条件检测功能饮料中牛磺酸的含量。方法 采用配备紫外检测器的高效液相色谱仪,利用C_18反相色谱柱,选择甲醇:乙酸钠=45:55(V:V)为流动相,在流速为0.8 mL/min、标液与衍生试剂比例为1:1(V:V)、衍生时间为1 h时进行检测。结果 在1.0~200μg/mL范围内,牛磺酸的浓度与峰面积呈现良好的线性,标准曲线方程是Y=34182X+14599,r~2=0.9999,优化后的方法回收率在96.83%~100.17%之间,检出限是0.53mg/100g,定量限为1.76mg/100g。结论 本方法高效、准确,适用于能饮料中牛磺酸含量的检测,为日常监管工作提供理论支持。 相似文献
2.
Ji Eun Du Young Ah You Eun Jin Kwon Soo Min Kim Jeongae Lee Ki Hwan Han Young Ju Kim 《International journal of molecular sciences》2020,21(23)
Fetal programming implies that the maternal diet during pregnancy affects the long-term health of offspring. Although maternal diet influences metabolic disorders and non-alcoholic fatty liver disease in offspring, the hepatic mechanisms related to metabolites are still unknown. Here, we investigated the maternal diet-related alterations in metabolites and the biological pathway in male offspring at three months of age. Pregnant rats were exposed to 50% food restriction during the prenatal period or a 45% high-fat diet during the prenatal and postnatal periods. The male offspring exposed to food restriction and high-fat diets had lower birth weights than controls, but had a catch-up growth spurt at three months of age. Hepatic taurine levels decreased in both groups compared to controls. The decreased hepatic taurine levels in offspring affected excessive lipid accumulation through changes in hepatocyte nuclear factor 4 A methylation. Moreover, the alteration of gluconeogenesis in offspring exposed to food restriction was observed to a similar extent as that of offspring exposed to a high fat diet. These results indicate that maternal diet affects the dysregulation in hepatic metabolism through changes in taurine levels and HNF4A methylation, and predisposes the offspring to Type 2 diabetes and non-alcoholic fatty liver disease in later life. 相似文献
3.
Recently, intensive processing of marine resources has attracted considerable attention. In order to further utilize the byproducts of aquatic shellfish (Pinctada martensii meat) with high value, this study proposes a method of extracting zinc and taurine from P. martensii meat. Zinc was first extracted from P. martensii meat with an ultrasonic crusher, and then taurine was further extracted by ultrasonic-assisted water extraction from the remaining shellfish. After optimization, the biological zinc extraction rate reached 8.63%, and the taurine extraction rate reached 0.71%. Meanwhile, the parameters for cation exchange separation and taurine purification were optimized, in which the injection volume, pH value, and elution rate were set to 8.0 mL, 4.5, and 3.0 mL/min, respectively. Ultimately, the purity the extracted taurine reached 98.16%. This study provides a novel method for the extraction of biological zinc and taurine by deep processing of shellfish meat. 相似文献
4.
刘和军 《食品安全质量检测学报》2021,12(12):4953-4958
牛磺酸是一种含硫条件性必需氨基酸,是人和动物机体内不可缺少的一种生理活性物质。牛磺酸对于机体的运动能力有着重要的影响,本文在介绍牛磺酸的代谢过程及生理作用的基础上,对目前国内外关于补充牛磺酸与提高人体的运动能力的关系、牛磺酸的安全性及副作用等方面研究情况做一综述。牛磺酸已开发成颇具潜力的抗运动性疲劳的营养补充剂,同时在医药、卫生、保健等领域里也具有广泛商业价值和社会价值。 相似文献
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目的建立全自动氨基酸分析仪测定支链氨基酸粉中牛磺酸。方法样品经上样缓冲液(pH 2.2)溶解提取,按照氨基酸分析仪设定的钠体系程序洗脱,用Na型离子交换色谱柱分离,与茚三酮反应液在135℃下反应,生成可在570 nm和440 nm检测到的蓝色物质,采用外标法定量。结果牛磺酸在4.1065~20.5326μg/mL浓度范围内有良好的线性,r~2=0.999,回收率为101.28%,RSD为2.1%,检出限为15 mg/100 g。结论该方法准确可靠,不需要剧毒衍生试剂,适用于支链氨基酸粉中牛磺酸的测定。 相似文献
8.
目的研究复方牛磺酸维生素饮料缓解小鼠体力疲劳的作用。方法将200只健康雄性ICR小鼠分成4组,第1组负重游泳,第2组测肝糖原和肝脏丙二醛(hepatic malondialdehyde,MDA),第3组测高尿素模型小鼠血清尿素氮(urea nitrogen,UREA)、乳酸脱氢酶(lactate dehydrogenase,LDH)、肝脏MDA,第4组测血乳酸(blood lactic acid,BLA),每组50只,组内按体重分成去离子水阴性对照组、糖水对照组、3个复方牛磺酸维生素饮料剂量组[3.33、6.67、20.00 mL/(kg·d) 6.25倍浓缩液]。各组连续灌胃30 d后,分别检测小鼠负重游泳时间、肝糖原、肝脏MDA、血清UREA、LDH、BLA等疲劳相关的指标。结果复方牛磺酸维生素饮料对各组小鼠体重无影响(P 0.05),在中剂量能显著延长小鼠负重游泳时间(P 0.05),显著增加小鼠肝糖原含量(P0.05),显著降低高尿素模型小鼠血清LDH(P0.01),在高剂量能显著降低高尿素模型小鼠血清中UREA(P0.01)、血清LDH(P0.05)、肝脏MDA含量(P0.05),对BLA曲线下面积无影响(P0.05)。结论此配方的复方牛磺酸维生素饮料对ICR小鼠具有缓解体力疲劳的功能。 相似文献
9.
Chaoyue Wen Fengna Li Lingyu Zhang Yehui Duan Qiuping Guo Wenlong Wang Shanping He Jianzhong Li Yulong Yin 《Molecular nutrition & food research》2019,63(2)
Energy metabolism is a basic and general process, by which the body acquires and uses energy to maintain normal function, and taurine plays a vital role in energy metabolism. Taurine deficiency may cause a weak energy metabolism and energy metabolism dysfunction. Taurine biosynthetic ability is limited, and its supplementation in the diet can strengthen energy metabolism in muscle performance, cardiac function, liver activity, and adipose tissue. Combining taurine with other drugs may have a superior effect in energy metabolism. In many metabolic disorders, taurine, or the combination of taurine with other drugs, also functions as a repair treatment for damaged tissues, and acts as a promoter for the balance of energy metabolism. The present study discusses the potential roles of taurine in energy metabolism. 相似文献
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