Fold and flexibility: what can proteins' mechanical properties tell us about their folding nucleus?

The determination of a protein''s folding nucleus, i.e. a set of native contacts playing an important role during its folding process, remains an elusive yet essential problem in biochemistry. In this work, we investigate the mechanical properties of 70 protein structures belonging to 14 protein families presenting various folds using coarse-grain Brownian dynamics simulations. The resulting rigidity profiles combined with multiple sequence alignments show that a limited set of rigid residues, which we call the consensus nucleus, occupy conserved positions along the protein sequence. These residues'' side chains form a tight interaction network within the protein''s core, thus making our consensus nuclei potential folding nuclei. A review of experimental and theoretical literature shows that most (above 80%) of these residues were indeed identified as folding nucleus member in earlier studies.     

Insertional re-activation of a chloramphenicol acetyltransferase misfolding mutant protein

The deletion of nine residues from the C-terminus of the bacterialchloramphenicol acetyltransferase (CAT) results in depositionof the mutant protein in cytoplasmic inclusion bodies and lossof chloramphenicol resistance in Escherichia coli. This foldingdefect is relieved by C-terminal fusion of the polypeptide withas few as two residues. Based on these observations, efficientpositive selection for the cloning of DNA fragments has beendemonstrated. The cloning vector encodes a C-terminally truncatedCAT protein. Restriction sites in front of the stop codon allowthe insertion of target DNA, resulting in the production ofproperly folded CAT fusion proteins and regained chloramphenicolresistance. The positive selection of recombinants is accomplishedby growth of transformants on chloramphenicol-containing agarplates. The method appears particularly convenient for the cloningof DNA fragments amplified by the PCR because minimal informationto restore CAT folding can be included in the primers. The cloningof random sequences shows that the folding defect can be relievedby fusion to a wide variety of peptides, providing great flexibilityto the positive selection system. This vector may also contributeto the determination of the role of the C-terminus in CAT folding.     

电缆气压保气率计算机统计系统

介绍了电缆气压保气率计算机统计系统开发的目的和意义，系统程序的流程和结构，以及系统程序的编制和应用。     

广播发射集散控制系统

本文介绍了广播发射台利用计算机技术、网络技术、遥控遥测技术、现代测试手段，对设备进行自动化控制与管理；实现设备技术指标自动测试，发射台无人值班、有人留守，建立机器的计算机档案制度，确保安全优质播出的方法和经验。     

二阶A型Cooper变换的增强型

对二阶Ａ型Ｃｏｏｐｅｒ变换进行增强，放宽可用性条件，并证明了其正确性，最后举例说明其应用。     

Modified atmosphere packaging of a mixed prepared vegetable salad dish

The aim of this study was to design a modified atmosphere package for a mixed vegetable salad consisting of 75 g of cut carrot, 55 g of cut cucumber, 20 g of sliced garlic and 50 g of whole green pepper. Respiration data of all the components were combined with film permeability data to predict package atmospheres and design optimal packages for experimental testing for improved shelf-life of the produce. The optimal package avoided minimum O₂ and maximum CO₂ tolerance limits, and chilling injury temperatures for any component. A pouch form package made of 27 mm low density polyethylene developed a modified atmosphere of 2.0–2.1% O₂ and 5.5–5.7% CO₂, which was beneficial for all components and provided better quality retention than other test packages.     

一种双光束自动测试光栅绝对与相对光谱衍射效率的原理性方案

提出了一种快速自动测试光栅光谱衍射效率的新方法。重点推导了测试平面光栅绝对光谱衍射效率与相对光谱衍射效率公式，介绍了双光束高精度自动测试光栅光谱衍射效率曲线的基本原理、方案与方法，并分析了测试精度。研究表明，本方案的测试速度和精度均比一般测试方法提高一倍，并能有效地消除光源波动对测量结果的影响，测量误差小于５×１０－３。     

Enhanced Image Detection on an ARM based Embedded System

This paper presents a new technique for thedetection of Integrated Circuits within images of Printed Circuit Boards autonomouslyand without the need to be assisted by CAD data. The technique is a key partof a suite of algorithms targeted for an embedded System On Chip architecturebased on the ARM7 platform for real time detection of PCB images for diagnosticpurposes. The technique has a significant reduction in complexity when comparedto conventional approaches such as the Hough Transform. The reduction in complexitymakes the approach ideal for an embedded vision application suchas the one described in this paper. This paper presents the technique, thetarget embedded architecture and results showing the reduction in complexitywhen compared to a Hough Transform.     

弹药包装冲击强度试验方法的改进研究

介绍一种新的弹药包装冲击强度试验方法，应用该试验方法可以防止冲击强度试验时出现与期望值相反的现象。     

广义预测控制系统的仿真软件设计与开发

介绍了基于广义预测控制的模型预测控制（MPC）系统仿真软件的开发与实现过程。该软件包含了MAC，IMAC，DMC，DDMC和GPC五种预测控制算法，可以用不同的方法实现对预测控制系统的综合性仿真以及控制器参数的调整。利用该软件可对模型预测控制系统的作用机理做进一步仿真分析，并有助于定性分析各种算法中设计参数对系统性能的影响以及不同算法之间的区别。