Designing amorphous formulations of polyphenols with naringin by spray-drying for enhanced solubility and permeability

Naringin (NAR), a major flavanone (FVA) glycoside, is a component of food mainly obtained from grapefruit. We used NAR as a food additive to improve the solubility and permeability of hydrophobic polyphenols used as supplements in the food industry. The spray-dried particles (SDPs) of NAR alone show an amorphous state with a glass transition temperature (T_g) at 93.2 °C. SDPs of hydrophobic polyphenols, such as flavone (FVO), quercetin (QCT), naringenin (NRG), and resveratrol (RVT) were prepared by adding varying amounts of NAR. All SDPs of hydrophobic polyphenols with added NAR were in an amorphous state with a single T_g, but SDPs of hydrophobic polyphenols without added NAR showed diffraction peaks derived from each crystal. The SDPs with NAR could keep an amorphous state after storage at a high humidity condition for one month, except for SDPs of RVT/NAR. SDPs with NAR enhanced the solubility of hydrophobic polyphenols, especially NRG solubility, which was enhanced more than 9 times compared to NRG crystal. The enhanced solubility resulted in the increased membrane permeability of NRG. The antioxidant effect of the hydrophobic NRG was also enhanced by the synergetic effect of NAR. The findings demonstrated that NAR could be used as a food additive to enhance the solubility and membrane permeability of hydrophobic polyphenols.     

Effect of hexagonal structure nanoparticles on the morphological performance of the ceramic scaffold using analytical oscillation response

Porous bony scaffolds are utilized to manage the growth and migration of cells from adjacent tissues to a defective position. In the current investigation, the effect of titanium oxide (TiO₂) nanoparticles on mechanical and physical properties of porous bony implants made of polymeric polycaprolactone (PCL) is studied. The bio-nanocomposite scaffolds are prepared with composition of nanocrystalline hydroxyapatite (HA) and TiO₂ powder using the freeze-drying technique for different weight fractions of TiO₂ (0 wt%, 5 wt%, 10 wt%, and 15 wt%). In order to identify the microstructure and morphology of the fabricated porous bio-nanocomposites, the X-ray diffraction (XRD), atomic force microscope (AFM) and scanning electron microscopy (SEM) are employed. Also, the biocompatibility and biodegradability of the manufactured scaffolds are examined by placing them in a simulated body fluid (SBF) for 21 days, their weight and pH changes are measured. The rate of degradation of the PCL-HA scaffold can be controlled by varying the percentage of its constituent components. Due to an increasing growth and activity of bone cells and the apatite formation on the free surface of the fabricated bio-nanocomposite implants as well as their reasonable mechanical properties, they have the potential to be used as a bone substitute. Additionally, with the aid of the experimentally extracted mechanical properties of the scaffolds, the vibrational characteristics of a beam-type implant made of the proposed porous bio-nanocomposites are explored. The results obtained from SEM image indicate that the scaffolds produced by the employed method have high total porosity (70%–85%) and effective porosity. The pore size is obtained between 60 and 200 μm, which is desirable for the growth and propagation of bone cells. Also, it is revealed that the addition of TiO₂ nanoparticles leads to reduce the rate of dissolution of the fabricated bio-nanocomposite scaffolds.     

山药复合营养面条加工工艺研究

以新鲜山药泥、小麦粉为主要原料,牛肉、胡萝卜、脱脂纯牛奶和食盐为辅料,制备一款营养丰富、无添加剂的新型山药复合营养面条。通过单因素试验、正交试验和感官评价,筛选山药复合营养面条配方;通过物性指标、微观结构确定山药复合营养面条的最佳配方为:山药泥35%、牛肉∶胡萝卜质量比为1∶1、脱脂纯牛奶3%和食盐2.5%。     

基于荧光显微镜的毛细管电泳系统的构建

毛细管电泳仪具有灵敏度高、分析速度快等优势,为降低其生产成本,基于电泳原理,以荧光显微镜为基础,设计了一套毛细管电泳系统。以20 bp(base pairs,碱基对)DNA ladder和100 bp DNA ladder为样本,全面分析了系统的稳定性、灵敏度和分离效果。结果表明:该系统在9 min内可以实现1500 bp以内DNA片段的高效分离,系统检测极限为0.1 ng/μL;在优化的电泳条件下,对限制性内切酶φX174-HincⅡ作用过的λ-DNA片段5 min内实现了291 bp与297 bp DNA片段的区分。     

不同改良措施对排土场土壤微形态影响的试验研究

以铁矿排土场废石土为研究对象,自制模型试验装置,设计4种基质改良方法,开展SEM检测实验,并应用IPP软件对SEM照片进行定量化分析,研究在不同改良措施下对铁矿排土场土壤微结构的影响。结果表明：单施3%有机肥土壤孔隙度为16%,与对照组排土场原矿土相比增加了60%。复合施用3%有机肥、掺拌30%的黄土的处理孔隙度达到最大33%。单施3%有机肥、5%有机肥对土壤大团聚体含量皆有显著的促进作用,复合施有机肥、掺拌黄土的处理与单施有机肥的处理对比,土壤大团聚体含量增加并不显著。各改良措施的MWD与FMD均随着土壤中大团聚体的含量增加而增加。     

电子束诱导沉积钴微米线及其铁磁性

通过电子束诱导沉积的方法制备了钴(Co)微米线,并利用扫描电子显微镜(SEM)、原子力/磁力显微镜(AFM/MFM)以及物性测量系统(PPMS)等手段对Co微米线的沉积尺寸、微结构、铁磁性和电学性质进行了测试和分析。研究结果表明:Co微米线轮廓清晰、均匀性好。在不同的沉积条件下,微米线的实际长度与设定长度基本一致;实际宽度数据呈类梯形分布,半高宽是设定值的2~10倍;实际厚度低于设定厚度的60%。沉积电流对Co微米线的铁磁特性有重要影响。当沉积电流大于0.5 nA时,样品呈现出良好的铁磁特性。另外,电学性能测试结果显示Co微米线呈现绝缘特性。成功制备了室温铁磁绝缘Co微米线,这将有助于深入开展微纳尺度的结构与器件的研究和应用。     

Improved direct inverse tracking control of a piezoelectric tube scanner for high-speed AFM imaging

For a piezoelectric tube scanner (PTS), this paper proposes an improved direct inverse tracking control algorithm and apply it to an atomic force microscope (AFM) to accomplish high-speed scanning tasks. That is, to enhance the high-speed tracking control performance of a PTS, an improved direct inverse rate-dependent Prandtl–Ishlinskii (P–I) model is firstly constructed, which includes a polynomial module to eliminate the structure nonlinearity. Based on the model, a practical feedforward control law is then designed to implement high-speed tracking control for a high-frequency trajectory with strong robustness, which presents the advantages of high-speed response, simple structure and convenient implementation. Subsequently, the designed feedforward law is combined with a feedback component, and the combined control strategy is employed in an AFM to accomplish fast imaging tasks. Numerous experimental results are then collected, which convincingly demonstrate the superior performance of the proposed practical model/control scheme.     

探讨仪器分析课程中扫描隧道显微镜演示实验

为了加强本科学生对"中级仪器分析"(双语)课程中的原子和分子尺寸概念的理解,通过演示实验(DEMO)对作为可视化工具的扫描隧道显微镜(STM)的基本原理进行了简要介绍。该DEMO实验配有学生对给定物质进行仪器分析的实际操作,以及从原子和分子水平的计算。在国际合作环境中,我们对实验和计算的细节进行了完善,并帮助学生建立明确的分子和原子概念。最后以分析高度有序的热解石墨(HOPG)的形貌特征作为DEMO和计算演示的例子。     

扫描隧道显微镜钨针尖的制备与表征

采用直流电化学刻蚀方法制备扫描隧道显微镜钨针尖,研究了电化学刻蚀过程中NaOH溶液浓度、钨丝浸入长度和刻蚀电压对针尖形貌的影响。通过扫描电子显微镜(SEM)测量针尖曲率半径和针尖纵横比值,以表征针尖的尺寸和形状;通过能谱仪(EDS)分析针尖表面成分,以表征表面清洁度;通过场发射显微镜(FEM)得到Fowler-Nordheim (F-N)曲线来检测针尖发射性能。实验结果表明,当溶液浓度为2 mol/L、钨丝浸入长度为4 mm、刻蚀电压为3 V时,可以得到曲率半径约为100 nm、纵横比值为13的针尖,且表面无钨的氧化层。FEM结果显示当对针尖施加500 V的负偏压时,针尖可以稳定发射50 nA量级的电流,且针尖性能具有良好的一致性。     

水中产气荚膜梭菌在不同过滤培养体系中的表型特征的研究

目的比较水中的产气荚膜梭菌在2种不同孔径滤膜和2种培养基上的计数结果,并探讨其机理。方法用0.22μm和0.45μm 2种不同孔径的滤膜过滤添加产气荚膜梭菌的矿泉水后,将滤膜贴在亚硫酸盐-多粘菌素-磺胺嘧啶琼脂培养基(sulfite-polymyxin-sulfadiazine agar,SPS)和胰胨-亚硫酸盐-环丝氨酸琼脂培养基(tryptose sulfite cycloserine agar,TSC)上,36℃±1℃厌氧培养24 h,观察菌落形态并进行菌落计数。用扫描电镜观察0.22μm和0.45μm2种滤膜表面的开口大小。结果水样经过2种不同孔径的滤膜过滤后,产气荚膜梭菌在TSC和SPS上的计数结果无显著性差异(P0.05)。但与SPS相比,产气荚膜梭菌在TSC上更易形成特征性黑色菌落;与孔径为0.22μm滤膜相比,在孔径为0.45μm滤膜上生长的菌落更大。电镜扫描发现,0.22μm滤膜和0.45μm滤膜的平均表面开口分别为2 5μm、4 0μm,后者的表面开口更大。结论水样通过0.45μm孔径滤膜过滤,贴在TSC琼脂上培养,更适用于矿泉水中产气荚膜梭菌的检验。