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1.
光学显微镜自动聚焦的技术研究   总被引:5,自引:5,他引:22  
在对普通光学显微镜进行一定改装的基础之上 ,设计了一套显微镜自动聚焦系统 ,并且给出了图像的灰度差分绝对值之和算子 ,解决了自动聚焦过程中是否正确聚焦的判断问题。最后还给出了一套基于启发式搜索算法的聚焦搜索策略 ,以加快焦平面的搜索。  相似文献
2.
提出了基于小波变换的图像清晰度评价函数。采用大NA(数值孔径)和小NA的显微图像序列,比较分析了本文提出的评价函数和经典的归一化方差函数、熵函数、能量拉普拉斯函数以及另外两种基于小波变换评价函数的清晰度评价性能。同时采用带有标准偏差为25的高斯噪声显微图像序列,比较了这五种评价函数的抗噪能力。实验结果表明:提出的评价函数具有最高的聚焦精度和聚焦分辨率,且具有与抗噪能力最强的归一化方差函数相当的抗噪能力。提出了基于区域选择的自动聚焦方法,实现了处于不同深度的微操作对象的3-D自动聚焦。该评价函数和区域选择聚焦技术可以用于高精度的自动微操作作业中。进一步说明自动调焦是实现自动化微操作的关键技术,而其核心是清晰度评价函数的选取或构建。  相似文献
3.
基于窗口模式的自动聚焦算法   总被引:1,自引:1,他引:0  
李成  李旭宇 《光学仪器》2009,31(2):52-56
随着数字智能化技术的不断成熟与提高,自动聚焦技术也日益完善并广泛应用。简单介绍了目前一些经典的图像清晰度评价函数,并提出一种改进的拉普拉斯算子的图像清晰度评价函数。该算法把整幅图像分成不同的窗口,提出一个新的概念——兴趣参数,并引入至不同窗口。经过与其它一些经典算法进行大量的实验研究对比,证明该算法在聚焦的适时性、灵敏性和准确性上都具有一定的优越性。  相似文献
4.
Reliable autofocusing is a critical part of any automated microscopy system: by precisely positioning the sample in the focal plane, the acquired images are sharp and can be accurately segmented and quantified. The three main components of an autofocus algorithm are a contrast function, an optimization algorithm and a sampling strategy. The latter has not been given much attention in the literature. It is however a very important part of the autofocusing algorithm, especially in high content and high throughput image-based screening. It deals with the problem of sampling the focus surface as sparsely as possible to reduce bleaching and computation time while with sufficient detail as to permit a faithful interpolation. We propose a new strategy that has higher performance compared to the classical square grid or the hexagonal lattice, which is based on the concept of low discrepancy point sets and in particular on the Halton point set. We tested the new algorithm on nine different focus surfaces, each under 24 different combinations of Signal-to-Noise ratio (SNR) and sampling rate, obtaining that in 88% of the tested conditions, Halton sampling outperforms its counterparts.  相似文献
5.
A new self-calibration method for a piezoelectric actuator-based vision-guided cell micromanipulation system is proposed. This method consolidates all the system parameters' uncertainties into a matrix instead of classifying into intrinsic and extrinsic. The position difference of the micromanipulator tip in the image plane between the measured and estimated output which is based on estimations of relevant parameters is assumed to be caused by the matrix. This matrix is estimated by means of collecting several pairs of known input and the corresponding output differences. Matrix standard deviation and gray-value based matching are applied to identify the output differences. Biological contamination is reduced since a calibration template is not required. This self-calibration is particularly suitable for cell micromanipulation systems where the micromanipulator is frequently dismounted and mounted.  相似文献
6.
A new simultaneous autofocus and twofold astigmatism correction method is proposed for High Angle Annular Dark Field Scanning Transmission Electron Microscopy (HAADF-STEM). The method makes use of a modification of image variance, which has already been used before as an image quality measure for different types of microscopy, but its use is often justified on heuristic grounds. In this paper we show numerically that the variance reaches its maximum at Scherzer defocus and zero astigmatism. In order to find this maximum a simultaneous optimization of three parameters (focus, x- and y-stigmators) is necessary. This is implemented and tested on a FEI Tecnai F20. It successfully finds the optimal defocus and astigmatism with time and accuracy, compared to a human operator.  相似文献
7.
分析了摄影环境的大气压力和温度的变化对航空相机离焦的影响,介绍了基于光学自准直的自动调焦系统的硬件电路原理和软件流程设计。基于光学自准直的检焦技术,选用两种图像清晰度评价函数,运用爬山搜索法采用变步长进行检焦控制,不仅有效地节省了检焦时图像处理的时间,而且提高了检焦的精度。通过引入高度和摄影角度数据,进行离焦量计算,通过控制驱动电路调整CCD的位置来完成自动调焦过程。经过光学测试和试飞证明,该系统满足了航空相机调焦精度要求。  相似文献
8.
为了有效地实现简单图像的快速高精度自动聚焦,提出了一种新的快速聚焦算法。首先在相机进行调焦时自动获取系列零件图像,并记录各图像对应的位置;然后计算每张图像中目标边缘的灰度变化跨度值,从中找到跨度值最小的图像,即为最清晰图像;最后把其所在位置反馈给硬件驱动系统,实现自动聚焦。用新算法分别对不同形状和不同材料的零件,在添加椒盐噪声和没有添加噪声的情况下进行实验,并与计算量小的几种经典最优聚焦函数做了对比试验。结果表明,用新算法对简单图像进行聚焦比常用的最优聚焦函数更敏锐,单峰性更好,抗噪能力更强,而且速度比最快的绝对梯度函数快30%以上。因此,新算法在拍摄简单场景时,鲁棒性好,可以更好地实现快速聚焦。  相似文献
9.
以长焦距斜视相机俯角系统为研究对象,针对高动态下自准直检焦镜筒定位精度不高的问题,提出了一种基于高增益观测器的多环路控制策略。首先,根据俯角控制系统开环幅频特性曲线,建立了俯角位置控制系统的数学模型,从控制理论角度分析了所设计控制策略的可行性;然后,通过实际系统进一步验证了所设计控制策略的可靠性。实验表明,高增益观测器能够很好地估计镜筒在非惯性空间下的角速度和角加速度状态;相比于双闭环控制方法,本文提出的控制策略可将俯角位置控制扰动最大误差减少96.3%,保证了镜筒的检焦位置定位精度优于0.05°,因此具有较好的工程应用价值。  相似文献
10.
For the microvision system, a new autofocus evaluation function based on the Robert function is proposed by increasing the threshold value. Compared with the traditional evaluation function, the new focus function reduces the local extreme value and increases the steepness of the focusing curve. According to the characteristics of the focusing evaluation function, the focus curve can be divided into two stages: the gentle area and the steep area. In the gentle area, there will be set a large step‐length to realize the fast search. In the steep area, the data will be fitted by Gauss method, and on the basis of the fitting results, the motor of microvision system was directly driven to achieve the focal plane and this method has been improved in real‐time and accuracy.  相似文献
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