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1.
航空成像像移模糊恢复技术   总被引:27,自引:18,他引:9  
贾平  张葆  孙辉 《光学精密工程》2006,14(4):697-703
分析了航空成像系统像移模糊产生的机理;通过建立运动模糊的数学模型,构建二维运动模糊点扩散函数;采用维纳滤波方法,消除了航空成像系统图像像移模糊;通过加窗技术,有效地抑制和减小了边缘误差.实验结果表明,该方法效果较好,可以实现1个像元的恢复精度.  相似文献
2.
The wavelength dependence of the incoherent point spread function in a wide-field microscope was investigated experimentally. Dispersion in the sample and optics can lead to significant changes in the point spread function as wavelength is varied over the range commonly used in fluorescence microscopy. For a given sample, optical conditions can generally be optimized to produce a point spread function largely free of spherical aberration at a given wavelength. Unfortunately, deviations in wavelength from this value will result in spherically aberrated point spread functions. Therefore, when multiple fluorophores are used to localize different components in the same sample, the image of the distribution of at least one of the fluorophores will be spherically aberrated. This aberration causes a loss of intensity and resolution, thereby complicating the localization and analysis of multiple components in a multi-wavelength image. We show that optimal resolution can be restored to a spherically aberrated image by constrained, iterative deconvolution, as long as the spherical aberration in the point spread function used for deconvolution matches the aberration in the image reasonably well. The success of this method is essentially independent of the initial degree of spherical aberration in the image. Deconvolution of many biological images can be achieved by collecting a small library of spherically aberrated and unaberrated point spread functions, and then choosing a point spread function appropriate for deconvolving each image. The co-localization and relative intensities of multiple components can then be accurately studied in a multi-wavelength image.  相似文献
3.
分析了航空成像系统运动模糊产生的机理,通过建立运动模糊数学模型,进行了消除运动模糊仿真实验。研究表明,由于成像系统与地面目标景物的相对运动,在目标图像上产生运动模糊,图像复原技术可以消除图像模糊。在图像复原技术中,点扩散函数(PSF)是影响图像恢复结果的关键因素,文中给出了估计点扩散函数的一般方法。虽然维纳滤波可以解决在逆滤波中H(u,v)零点噪声放大问题,但是在图像边缘附近误差较大。采用带最优窗的维纳滤波方法可有效抑制噪声和减小边缘误差。对实验平台获取的运动模糊图像进行实验,结果表明在图像边缘像素灰度平滑条件下,可以收到近乎完美的恢复效果。  相似文献
4.
改进的奇异值分解法估计图像点扩散函数   总被引:8,自引:8,他引:0  
为了提高图像复原算法的性能,提出了一种改进的奇异值分解法估计图像的点扩散函数。从图像的退化离散模型出发,对图像进行逐层分块奇异值分解,并自动选取奇异值重组阶数以减少噪声对估计的影响。利用理想图像奇异值向量平均能谱指数模型,估计点扩散函数奇异值向量的频谱,再反傅里叶变换得到其时域结果。实验结果表明,该方法能在不同信噪比情况下估计成像系统的点扩散函数,估计结果比原有估计方法有所提高,有望为图像复原算法的预处理提供一种有效的手段。  相似文献
5.
位相复原技术在光学成像质量测评中的应用   总被引:5,自引:4,他引:1  
对于大口径或特殊波段成像的光学系统,采用常规测试手段难以实现对光学系统成像质量的评估。而光学系统波像差是表征光学系统成像质量的重要指标之一。本文基于信息光学基础理论采用傅立叶变换和迭代算法通过对光学成像系统星点图像(光强点扩散函数PSF)计算分析,从而实现对光学成像系统位相的复原获得波像差信息。本文论述了有关的理论依据和分析计算公式;并研制了位相复原分析计算软件。通过计算机仿真与误差分析论证了研制的位相复原计算方法的正确性,复原误差小于5%。提出了在实际应用中采用此方法时减少复原误差的一种途径。通过实际采集的光学系统星点图像的位相复原实验验证了位相复原分析软件的适用性。此方法为非常规光学成像系统波相差的评估工作提供了新途径。  相似文献
6.
The role of specimen-induced spherical aberration in confocal microscopy   总被引:4,自引:1,他引:3  
We present an overview of recent theories for describing specimen-induced spherical aberration in confocal microscopy. One of these theories is used to compute numerically the role of spherical aberration in general confocal, and especially in biological confocal, microscopy for a variety of three-layer specimen structures. In particular, we study the effect of specimen-induced spherical aberration on the maximum value of the overall confocal point spread function, the accompanying focal shift and the size of the optical probe in both fluorescence and brightfield confocal microscopy.  相似文献
7.
Effects of specimen refractive index on confocal imaging   总被引:3,自引:0,他引:3  
The aberrations introduced when focusing within a specimen with a refractive index equal to that of water using an oil-immersion objective are investigated theoretically. The peak intensity in the confocal point spread function drops by a factor of two for focusing less than 10 μm into the specimen. The effects on scaling of dimensions in the resulting images are discussed. The image exhibits an axial stretching by a factor of about 1.12.  相似文献
8.
For deconvolution applications in three-dimensional microscopy we derived and implemented a generic, accelerated maximum likelihood image restoration algorithm. A conjugate gradient iteration scheme was used considering either Gaussian or Poisson noise models. Poisson models are better suited to low intensity fluorescent image data; typically, they show smaller restoration errors and smoother results. For the regularization, we modified the standard Tikhonov method. However, the generic design of the algorithm allows for more regularization approaches. The Hessian matrix of the restoration functional was used to determine the step size. We compared restoration error and convergence behaviour between the classical line-search and the Hessian matrix method. Under typical working conditions, the restoration error did not increase over that of the line-search and the speed of convergence did not significantly decrease allowing for a twofold increase in processing speed. To determine the regularization parameter, we modified the generalized cross-validation method. Tests that were done on both simulated and experimental fluorescence wide-field data show reliable results.  相似文献
9.
10.
一种基于DFD的自动对焦算法   总被引:2,自引:1,他引:1  
引入了一种基于DFD的自动对焦算法。通过这种算法,只要给定了两幅不同离焦位置的图像,就可由算法推导出目标物体的正确对焦位置,从而控制镜头完成自动对焦。实验表明,这种算法精度比较高,速度比较快,鲁棒性良好。  相似文献
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